Gd<3+>-induced self-assembly method of gold nanoclusters for forming gold nanoparticles and applications of gold nanoparticles

A technology of gold nanoclusters and gold nanoparticles, which is applied in the field of self-assembly products of gold nanoclusters induced by trivalent gadolinium ions Gd3+, multimodal imaging of tumors, and can solve the problem of no fluorescence imaging, no interaction, and complex steps Many problems, to achieve the effect of reducing synthesis cost, high biocompatibility, and good relaxation time

Inactive Publication Date: 2016-11-09
SHANGHAI JIAO TONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The method is complicated and has many steps, and there is no direct interaction between gold nanoparticles and gadolinium ions
And although CT / MRI dual-modal imaging can be used for diagnosis, it does not have the function of fluorescence imaging
[0007] The search results show that: trivalent gadolinium ion (Gd 3+ )-induced self-assembly of gold nanoclusters for multimodal imaging of tumors, which has not been reported yet

Method used

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  • Gd&lt;3+&gt;-induced self-assembly method of gold nanoclusters for forming gold nanoparticles and applications of gold nanoparticles
  • Gd&lt;3+&gt;-induced self-assembly method of gold nanoclusters for forming gold nanoparticles and applications of gold nanoparticles
  • Gd&lt;3+&gt;-induced self-assembly method of gold nanoclusters for forming gold nanoparticles and applications of gold nanoparticles

Examples

Experimental program
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Effect test

Embodiment 1

[0041] (1) Preparation of gold nanoclusters: freshly prepared HAuCl 4 The solution (8mM / mL) and GSH solution (150mM / mL) were added to 43.5mL of cold ultrapure water, and stirred in an ice bath (200rpm / min) for 3min, forming a white Au(I)-SG insoluble matter. The cold tetrabutylammonium bromide (TBAB) solution (molar ratio: TBAB / Au=9.3:1) was added to the above mixture and stirred vigorously for 5 minutes and then allowed to stand for 2 hours. Adjust the pH value to ≈3.0 with 1M HCl, and continue to ice bath for 12h to form gold nanoclusters.

[0042] (2) Purification of gold nanoclusters: centrifugation to remove the insoluble Au(I)-SG complex, ultrafiltration and concentration, adding NaCl and methanol to precipitate the gold nanoclusters, freeze-drying and put it at 4°C for use.

[0043] For the gold nanoclusters prepared above, in the subsequent self-assembly step, the negative charge of the carboxyl group on the surface of the gold nanocluster can interact with the positive cha...

Embodiment 2

[0045] (1) Trivalent Gd 3+ Preparation: Gadolinium Chloride Hexahydrate (GdCl 3 ·6H 2 O) Dissolve in deionized water with pH≈6.5, prepare an aqueous solution with a final volume of 0.5 mL according to the required amount, and use 100W ultrasound for 2 minutes before use.

[0046] (2) Trivalent gadolinium ion (Gd 3+ ) Induced self-assembly of gold nanoclusters: First, the gold nanoclusters prepared in Example 1 were dissolved in pH≈6.5 (6.2 3+ (Gd 3+ The molar ratio of ion to gold nanocluster is 1.26:1) Slowly add dropwise (about 400μL / min) into the gold nanocluster aqueous solution, vortex rapidly at 1500rpm / min for 30s-1min, and then place it in a water bath at 25-30℃ Heating, stirring at 1300 rpm / min for 3h-6h, to obtain assembled gold nanoparticles.

Embodiment 3

[0048] (1) Trivalent Gd 3+ Preparation: Gadolinium Chloride Hexahydrate (GdCl 3 ·6H 2 O) Dissolve in deionized water with pH 7.0, make up an aqueous solution with a final volume of 0.5 mL according to the required amount, and ultrasonicate at 120W for 3 minutes before use.

[0049] (2) Trivalent gadolinium ion (Gd 3+ ) Induced self-assembly of gold nanoclusters: first dissolve gold nanoclusters in deionized water (2.5mg, 2mL) at pH 7.0, and then freshly prepare Gd 3+ (The molar ratio of Gd3+ ions to gold nanoclusters is 1.1:1) Slowly drop (about 350μL / min) into the gold nanocluster aqueous solution, vortex at 1600rpm / min for 1min, and then heat in a water bath at 25°C. Stir at 1400 rpm / min for 6 hours to obtain assembled gold nanoparticles.

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Abstract

The invention provides a Gd<3+>-induced self-assembly method of gold nanoclusters for forming gold nanoparticles and applications of the gold nanoparticles. The Gd<3+>-induced self-assembly method comprises the following steps: a preparation step of the gold nanoclusters, specifically comprising the following substeps: preparing the gold nanoclusters by adopting chiral L-form glutathione as a sulfydryl ligand, and dissolving the gold nanoclusters in deionized water, thus forming a solution A; a preparation step of trivalent gadolinium ions Gd<3+>, specifically comprising the following substeps: dissolving gadolinium chloride hexahydrate in deionized water, thus preparing a solution B, and carrying out ultrasonic treatment for later use; and a Gd<3+>-induced self-assembly step of the gold nanoclusters, specifically comprising the following substeps: dropwise adding the solution B into the solution A slowly, and inducing the gold nanoclusters to form the gold nanoparticles through self-assembly. The method is simple, the material synthesis cost is low, the reaction conditions are simple, the operation is easy, the prepared gold nanoparticles have the enhanced fluorescence imaging and CT imaging effects, Gd<3+> is chelated, and the gold nanoparticles can be taken as a nuclear magnetic imaging contrast agent of tumor. The experiment result shows that the prepared gold nanoparticles can be well applied to the field of multi-modal imaging of the tumor.

Description

Technical field [0001] The present invention relates to the technical field of inorganic nano materials, in particular, to Gd 3+ Induced gold nanoclusters self-assembly into gold nanoparticles method, and trivalent gadolinium ion Gd 3+ The induced self-assembly products of gold nanoclusters are used in multi-modal imaging methods of tumors. Background technique [0002] At present, with the acceleration of the pace of life and the deterioration of the environment, cancer has become the main killer threatening human health. Among them, the number of patients with lung cancer ranks first in the number of patients with malignant tumors. Finding an effective tumor diagnosis method is of great significance to safeguarding human life and health. In recent years, Optical Imaging (OI), Magnetic Resonance Imaging (MRI), Positron Emission Tomography (PET), Single Photon Emission CT (SPECT) and Ultrasonography (Ultrasonography, USG) has been widely used in the clinical diagnosis of tumors...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K49/04A61K49/18A61K49/06
CPCA61K49/0002A61K49/0019A61K49/0093A61K49/04A61K49/06A61K49/1818
Inventor 崔大祥侯文秀夏芳芳张春雷
Owner SHANGHAI JIAO TONG UNIV
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