Method for preparing fermented cottonseed protein by fermenting cottonseed protein and solid-state aerobic fermentation

A cottonseed protein and preparation technology, applied in fermentation, animal husbandry, hydrolytic enzymes, etc., can solve the problems of low fermentation efficiency and unoptimized raw materials, improve the breeding environment, and be conducive to the harmlessness and resource utilization of feces. The effect of enhancing disease resistance

Active Publication Date: 2019-12-13
四川伯禹生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In the prior art, there is also a general-purpose leavening agent, which has a simple process and is used for fermentation of various raw materials (such as soybean meal, cotton meal and dregs, etc.), but in fact it cannot be optimal for each raw material; , low fermentation efficiency, only suitable for sideline agricultural production
[0008] This field has not yet developed a better biological method that can decompose gossypol and anti-nutritional factors in cottonseed meal under a single substrate condition and improve protein solubility

Method used

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  • Method for preparing fermented cottonseed protein by fermenting cottonseed protein and solid-state aerobic fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0055] Example 1 Cotton Meal Screening Pretreatment Conditions

[0056] The cotton meal raw material is pulverized with an industrial pulverizer, and the sieve of the pulverizer is 2.0 mm. Sieve the crushed cottonseed meal with 10 mesh, 20 mesh, 30 mesh, 40 mesh, 60 mesh and 80 mesh screens respectively, and weigh the weight of the sieve and the sieve respectively. Among them, the 10-mesh sieve is all short fluff, accounting for about 2% of the total weight of raw materials; the 20-mesh sieve is short fluff and a small amount of cottonseed husk impurities, accounting for about 8% of the total raw material weight, and the under-sieve is not visible to the naked eye. The short fluff; from 30 mesh to 80 mesh sieving results, the cotton kernels in the sieve material increase with the finer sieve holes. Based on the above, 20-30 mesh sieving is selected as the pretreatment before fermentation to reduce the content of impurities such as fiber substances. Preferably 20 meshes, anal...

Embodiment 2

[0057] The preparation of embodiment 2 solid production bacterial classification

[0058] Pick 1 loop (inoculation loop) from the slant-preserved strain of Saccharomycopsis fibuligera, insert it into 100mL potato culture solution, and culture it in a shaker flask (180rpm) at 30°C for 52h to obtain Saccharomycopsis fibuligera. Hymenospora yeast culture medium.

[0059] Take 50g of yeast liquid of S. forticulata, add it into 5000g of sterilized bran corn culture medium (calculated as dry material), mix evenly, and cultivate at 30°C for 48h to obtain solid yeast of S. forticulata. strains.

[0060] Preparation of potato sucrose culture solution: Peel fresh potatoes and cut them into thumb-sized chunks, weigh 100g, add 500g of tap water, boil for 20 minutes, filter with gauze, discard the residue and take the supernatant, and dilute with tap water to 500g, then add 10g of sucrose, after melting, pack into 250ml Erlenmeyer flasks, 100g per bottle. Sterilization was maintained at...

Embodiment 3

[0064] The addition of embodiment 3 nutrient salts is on the impact of processing result

[0065] The cottonseed meal raw material is pulverized according to Example 1, and sieved with 20 mesh to obtain cottonseed meal powder. Then prepare mixed yeast solid strain according to Example 2.

[0066] Will KH 2 PO 4 , KCl, MgSO 4 , FeSO 4 , ZnSO 4Add phytase and phytase in groups according to the table, mix evenly, incubate at 30°C for 48 hours, and measure the cell number and crude protein content after fermentation.

[0067] 1 2 3 4 5 6 7 8 Cotton meal powder (g) 1000 1000 1000 1000 1000 1000 1000 1000 water (g) 700 700 700 700 700 700 700 700 Strains (g) 5 5 5 5 5 5 5 5 K H 2 PO 4 (g)

[0068] Select nutrient salt addition as the eighth group, namely: KCl 0.1%, MgSO 4 0.05%, FeSO 4 0.005%, ZnSO 4 0.0025%, phytase 0.02%. The decomposition of phytic acid in cotton meal by phytase can provide phosphorus sour...

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Abstract

The invention belongs to the technical field of microbial fermentation, particularly relates to fermented cottonseed protein and a method for preparing the fermented cottonseed protein through solid aerobic fermentation and aims to provide the fermented cottonseed protein. The fermented cottonseed protein is prepared from following raw materials in a weight ratio through fermentation: 97-99 parts of cottonseed meal, 0-1.5 parts of corn flour, 0.7-1.5 parts of solid strains of mixed saccharomycetes, 0.1-0.4 parts of mixed nutrients, 0.03-0.1 parts of a mixed enzyme preparation and water, wherein the total weight of water is 0.6-0.9 times that of the other five raw materials. The method for preparing the fermented cottonseed protein through the solid aerobic fermentation mainly comprises steps as follows: the raw materials are taken and mixed uniformly and are fermented in an aerobic state for 45-48 h at the temperature of 30-35 DEG C, and then a mixture is dried. With the adoption of the method, gossypol and anti-nutritional factors in the cottonseed meal can be effectively removed, and one better way is provided for effective utilization of the cottonseed meal.

Description

technical field [0001] The invention belongs to the technical field of preparing microbial fermentation, and in particular relates to a fermented cottonseed protein and a preparation method thereof through solid-state aerobic fermentation. Background technique [0002] At present, my country's aquaculture industry relies heavily on corn-soybean meal-based rations, especially soybean meal, the main protein raw material, most of which are imported and subject to the international market, often causing large fluctuations in breeding costs and increasing risks. In addition, imported soybean meal contains genetically modified ingredients, which do not meet the requirements for organic agricultural products. [0003] Cotton meal is a by-product of cottonseed oil extraction. It is rich in nutrients, with more than 40% protein, more balanced amino acid composition, and higher arginine content than soybean meal. The annual output of cottonseed meal in my country is about 6 million t...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/16C12N9/14C12P21/00A23K10/37A23K10/30A23K10/12A23K10/14A23K20/20A23K20/24
CPCY02P60/87
Inventor 邓小晨吴谦
Owner 四川伯禹生物工程有限公司
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