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High performance liquid chromatography detection method for tryptophan in fodder

A high-performance liquid chromatography and detection method technology, which is applied in the detection field of tryptophan, can solve the problems of low detection sensitivity, measurement interference, and long analysis time, so as to reduce background interference, improve separation conditions, and facilitate hydrolysis conditional effect

Inactive Publication Date: 2016-10-26
四川威尔检测技术股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The present invention aims to solve the problems that the existing high-performance liquid chromatography method for determining tryptophan in feed has slow peak output, long analysis time, interference in the measurement and low detection sensitivity, and provides a method for detecting tryptophan in feed. High-performance liquid chromatography detection method has high sensitivity and precision, good reproducibility, fast, accurate qualitative and quantitative and high recovery rate, and can accurately analyze tryptophan in feed

Method used

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] A high-performance liquid chromatography detection method for tryptophan in feed, comprising the following steps:

[0026] A. Sample pretreatment

[0027] The feed samples were pulverized and put into microwave-assisted protein hydrolysis equipment for microwave-assisted protein hydrolysis; the microwave power was controlled at 600 W, and the temperature was controlled to rise from room temperature to 150 °C within 10 minutes, and then keep warm for 20 minutes; after the keep warm, The hydrolyzate is processed for detection by high performance liquid chromatography;

[0028] B. Preparation for HPLC analysis

[0029] Column: Syncronis C 18 Chromatographic column, 250×4.6 mm, 5 µm; flow rate: 1 mL / min; column temperature: 30 ℃; detector: DAD detector, detection wavelength: 220 nm; mobile phase: isocratic elution, 0.05 mol / L phosphoric acid Potassium dihydrogen solution + acetonitrile = 90 + 10 (V + V);

[0030] C, drawing of tryptophan detection standard curve

[003...

Embodiment 2

[0033] A high-performance liquid chromatography detection method for tryptophan in feed, comprising the following steps:

[0034] A. Sample pretreatment

[0035] The feed samples were pulverized and put into microwave-assisted protein hydrolysis equipment for microwave-assisted protein hydrolysis; the microwave power was controlled at 1000 W, the temperature was controlled to rise from room temperature to 170 °C within 30 minutes, and then kept for 40 minutes; after the heat preservation was completed, The hydrolyzate is processed for detection by high performance liquid chromatography;

[0036] B. Preparation for HPLC analysis

[0037] Column: Syncronis C 18 Chromatographic column, 250×4.6 mm, 5 µm; flow rate: 1 mL / min; column temperature: 30 ℃; detector: DAD detector, detection wavelength: 220 nm; mobile phase: isocratic elution, 0.05 mol / L phosphoric acid Potassium dihydrogen solution + acetonitrile = 90 + 10 (V + V);

[0038] C, drawing of tryptophan detection standard...

Embodiment 3

[0041] A high-performance liquid chromatography detection method for tryptophan in feed, comprising the following steps:

[0042] A. Sample pretreatment

[0043]The feed samples were pulverized and put into microwave-assisted protein hydrolysis equipment for microwave-assisted protein hydrolysis; the microwave power was controlled at 800 W, the temperature was controlled to rise from room temperature to 160 °C within 20 minutes, and then kept for 30 minutes; after the heat preservation was completed, The hydrolyzate is processed for detection by high performance liquid chromatography;

[0044] B. Preparation for HPLC analysis

[0045] Column: Syncronis C 18 Chromatographic column, 250×4.6 mm, 5 µm; flow rate: 1 mL / min; column temperature: 30 ℃; detector: DAD detector, detection wavelength: 220 nm; mobile phase: isocratic elution, 0.05 mol / L phosphoric acid Potassium dihydrogen solution + acetonitrile = 90 + 10 (V + V);

[0046] C, drawing of tryptophan detection standard c...

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Abstract

The invention relates to a high performance liquid chromatography detection method for tryptophan in fodder and belongs to the technical field of tryptophan detection. The detection method comprises the steps of specially pretreating a sample, preparing HPLC analysis and drawing a tryptophan detection standard curve. The detection method has higher flexibility and accuracy, is low in detection limit, better in repeatability, quick, accurate in qualitativeness and quantitation and higher in recovery rate, can accurately analyze tryptophan in the fodder and can be applied to large-scale sample detection and suitable for standardization.

Description

technical field [0001] The invention relates to a detection method of amino acids in feed, more specifically, the invention relates to a high-performance liquid chromatography detection method of tryptophan in feed, belonging to the technical field of tryptophan detection. Background technique [0002] Tryptophan usually exists widely in organisms in a free or bound state. It is one of the eight essential amino acids for humans and animals. As a functional amino acid, it plays an important role in animal body composition, feeding, immunity, and stress resistance. play an important role. In the study of livestock and poultry animals, it was found that adding tryptophan to low-protein feed can improve animal food intake, growth performance, promote digestion and absorption of nutrients, and obtain growth performance similar to high-protein feed. Tryptophan is very important to the growth and nitrogen balance of organisms. It can participate in the renewal of plasma proteins i...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 赵艳杨发树宋军张艳红张凤枰刘耀敏
Owner 四川威尔检测技术股份有限公司
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