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Abelmoschus manihot stem and leaf polysaccharide and preparation method and application thereof

A technology of hollyhock, stems and leaves is applied in the field of preparation of polysaccharides of stems and leaves of hollyhock, can solve the problems of waste of resources, lack of effective utilization methods of stem and leaf resources, abandonment and the like, achieves improvement of production efficiency, promotes proliferation of spleen lymphocytes, and facilitates enrichment. Effect

Active Publication Date: 2016-10-12
NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Hollyhock flower is currently one of the main raw materials of traditional Chinese medicine for the treatment of kidney disease. The market demand is huge, but the stem and leaf resources after harvesting the flowers are largely discarded due to lack of effective utilization methods, resulting in waste of resources.

Method used

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  • Abelmoschus manihot stem and leaf polysaccharide and preparation method and application thereof
  • Abelmoschus manihot stem and leaf polysaccharide and preparation method and application thereof
  • Abelmoschus manihot stem and leaf polysaccharide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Extraction process of hollyhock stem and leaf polysaccharide is preferred

[0030] (1) Optimization of single factor for extracting polysaccharides from stems and leaves of hollyhock

[0031] Take the hollyhock stem and leaf samples and divide them into several parts. After proper crushing, weigh them, add water with a weight ratio of (1:10, 1:15, 1:20, 1:30, 1:40) of the raw materials, and set the temperature at (60°C , 70°C, 80°C, 90°C, 100°C) extraction temperature, extraction (0.5h, 1h, 2h, 3h, 4h), extraction (1 time, 2 times, 3 times). Filtrate, take the supernatant, and measure the polysaccharide extraction rate by the phenol-sulfuric acid method, and the optimal factor index results are shown in Table 1.

[0032] Table 1 Univariate analysis

[0033]

[0034]

[0035] (2) Orthogonal optimization test for extraction of polysaccharides from stems and leaves of hollyhock

[0036] Taking solid-liquid ratio, extraction time, and extraction temperatu...

Embodiment 2

[0042] Embodiment 2 A kind of separation and purification process of hollyhock stem and leaf polysaccharide, it comprises the steps:

[0043] (1) Get 1.0 kg of hollyhock stems and leaves, after crushing, add 20 kg of water according to the optimized extraction method in Example 1, heat and reflux at 100° C. to extract twice, each time for 2 hours, and filter to obtain the filtrate.

[0044] (2) Take the filtrate obtained in step (1), concentrate it to 1 L, add ethanol until the alcohol content is 80%, let it settle for 24 hours, and centrifuge to separate the solid and liquid to obtain the alcohol precipitate.

[0045](3) Take the alcohol precipitation precipitate obtained in step (2), add water to dissolve, separate through AB-8 macroporous adsorption resin column chromatography, and elute with 10% ethanol aqueous solution to obtain the eluent.

[0046] (4) Take the eluate obtained in step (3), concentrate it appropriately, and ultrafilter it through a hollow cellulose ultraf...

Embodiment 3

[0048] Embodiment 3 A kind of separation and purification process of hollyhock stem and leaf polysaccharide, it comprises the steps:

[0049] (1) Get 2.0 kg of hollyhock stems and leaves, after crushing, add 40 kg of water according to the optimized extraction method in Example 1, heat and reflux at 100° C. to extract 3 times, each time for 1 hour, and filter to obtain the filtrate.

[0050] (2) Take the filtrate obtained in step (1), concentrate it to 1 L, add ethanol to reach an alcohol content of 70%, let it settle for 24 hours, and filter to obtain an alcohol precipitate.

[0051] (3) Take the alcohol precipitation precipitate obtained in step (2), dissolve it in water, separate it through D101 macroporous adsorption resin column chromatography, and elute with water to obtain an eluent.

[0052] (4) Take the eluate obtained in step (3), concentrate it properly, and ultrafilter it through an ultrafiltration membrane, take some samples with a molecular weight ranging from 10...

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Abstract

The invention discloses an abelmoschus manihot stem and leaf polysaccharide and a preparation method and an application thereof. The preparation method comprises the steps: extracting, carrying out ethanol precipitation, carrying out chromatography purification with a macroporous adsorption resin column, separating with a hollow cellulose membrane, carrying out chromatography separation with a DEAE cellulose resin column, collecting target fractions, carrying out desalting treatment, freeze-drying, and thus obtaining the abelmoschus manihot stem and leaf polysaccharide. The abelmoschus manihot stem and leaf polysaccharide has the weight-average molecular weight of 13821 Da and is composed of rhamnose, mannose, galactose, galacturonic acid, glucose and arabinose, and the molar ratio of rhamnose to mannose to galactose to galacturonic acid to glucose to arabinose is 1 to 0.53 to 0.12 to 0.05 to 0.16 to 0.03; the abelmoschus manihot stem and leaf polysaccharide has a good immune enhancing activity, and can be used for development of immune enhancing drugs and health food. Stem and leaf waste resources produced in a production process of abelmoschus manihot medicinal materials are used as raw materials, the polysaccharide substance having the immune activity is separated, and a basis is laid for cyclic utilization of the traditional Chinese medicine resources.

Description

technical field [0001] The invention relates to a traditional Chinese medicine extract, in particular to a preparation method and application of the stem and leaf polysaccharide of marshmallow with immune activity. Background technique [0002] Hollyhock (Abelmoschus manihot (L.) Medic.) is a one-year to perennial herb of the Malvaceae genus Abelmoschus. It was first recorded in "Jiayou Materia Medica" written by Zhang Yuxi in the Song Dynasty. All have records, and included in the past editions of "The Pharmacopoeia of the People's Republic of China". In the past dynasties, herbal medicines mostly used their flowers as medicine, followed by their seeds, and their roots, stems and leaves were rarely used. Hollyhock flower is currently one of the main raw materials of traditional Chinese medicine for the treatment of kidney disease. The market demand is huge, but the stem and leaf resources after harvesting the flowers are largely discarded due to lack of effective utilizati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61P37/04A23L33/105
CPCA23V2002/00A23V2200/324C08B37/0003C08B37/006
Inventor 郭盛段金廒钱大玮严辉
Owner NANJING UNIVERSITY OF TRADITIONAL CHINESE MEDICINE
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