Bacillus aryabhattai J5 and application thereof

A technology of Bacillus arborii and strains, applied in the field of microbial resources, can solve the problems of limited laboratory and pot experiment stage, and achieve good effect of pot experiment, increase of total particle number, and increase of ear length

Active Publication Date: 2016-10-05
SOUTH CHINA AGRI UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] As a dominant strain of Bacillus arseni as a new microbial agent, the c

Method used

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  • Bacillus aryabhattai J5 and application thereof
  • Bacillus aryabhattai J5 and application thereof
  • Bacillus aryabhattai J5 and application thereof

Examples

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Embodiment 1

[0038] The present invention will be further elaborated below in combination with the accompanying drawings and specific embodiments. The embodiments are only used to explain the present invention, and are not intended to limit the scope of the present invention. The test methods used in the following examples are conventional methods unless otherwise specified; the materials and reagents used are commercially available reagents and materials unless otherwise specified. Example 1 Isolation of bacterial strains

[0039] S1. Separation: Take the vegetable garden soil of Yuejin North Farm, South China Agricultural University, Tianhe District, Guangzhou City, Guangdong Province as the screening soil sample, accurately weigh 10.0g of the screening soil sample by the flat plate coating method, and put it into a 250mL triangle filled with 90mL sterile water. In the bottle (with glass beads), oscillate on a shaker for 30 minutes to fully disperse the cells, let it stand for 20-30 seco...

Embodiment 2

[0041] Embodiment 2 J5 strain identification

[0042] Morphological characteristics of bacteria: Gram-positive bacteria with spores. The bacteria are rod-shaped, motile, and obligately aerobic. See photos of spore staining figure 1 .

[0043] Colony Morphological Characteristics: The colonies formed after culturing on beef extract peptone medium for 24 hours are round or irregular. After 48 hours, the colonies are all round, slightly yellow, with a diameter of about 2-3mm, irregular edges, flat and moist.

[0044] Growth characteristics: In the liquid medium of 3.0 g of beef extract, 10.0 g of peptone, 5.0 g of sodium chloride, and 1L of water, the rotation speed was 113 rpm, the temperature was 30°C, and the initial pH value was 7.0, and it was cultivated for 18 hours. The number of live bacteria is (1.94±0.60)×10 8 cfu mL -1 .

[0045] Physiological and biochemical characteristics: Refer to the routine experimental methods in the "Bergey's Bacterial Identification Man...

Embodiment 3

[0049] Example 3 J5 bacteria produce auxin effect

[0050] Qualitative determination of auxin: Inoculate J5 bacteria in 50 mL of LB liquid medium containing L-tryptophan (100 µg / mL), culture at 30 °C, 150 r / min on a shaker for 24 h. Take 300 μL of culture solution and drop it on a white ceramic plate, add 300 μL of Salkowski colorimetric solution at the same time, set 300 μL of sterilized LB medium as a negative control, 10, 20, 30, 50 (µg / mL) auxin colorimetric solution as a positive control. Place the white ceramic plates at room temperature and under light-proof conditions for 30 minutes and then observe. Those that turn red indicate that auxin can be produced. See the test results Figure 4 .

[0051] Quantitative determination of auxin: J5 bacteria were inoculated in 50 mL LB liquid medium containing L-tryptophan (100 µg / mL), three replicates, 30 ° C, 150 r / min shaker culture for 24 h. Centrifuge the culture solution at 8000×g for 10 min, take 4 mL of the supernatant ...

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Abstract

The invention belongs to the field of microbial resources, and particularly discloses a strain of Bacillus aryabhattai J5 and application thereof in promoting growth of corn. The Bacillus aryabhattai J5 is collected in CGMCC (China General Microbiological Culture Collection Center) on October 15th, 2015 and is filed with the number of CGMCC NO.11508. The Bacillus aryabhattai J5 has functions of producing auxin, siderophore, ammonia and protease, dissolving phosphorus, resisting to salt, realizing antagonism, and the like. As the strain is capable of growing normally in a culture medium with salt content equal to 11%, the strain has good potential in eliminating soil salinization. As content of siderophore produced by the strain is up to 53.7%, the strain has effective disease-prevention property. By the strain, corn seed germination rate can be increased by 12.5%, and accordingly the strain has good growth promoting effect. By application of microbial inoculum prepared from the strain, corn yield is increased by 37.1%, and hundred-grain weight is increased by 35%. It is possible to develop the strain into microbial inoculum, and accordingly the strain has wide application prospect in terms of improving soil nutrient, eliminating salinization, promoting growth of plants, preventing diseases and the like.

Description

technical field [0001] The invention relates to the field of microbial resources, more specifically, to a strain of Bacillus arborii J5 and its application. Background technique [0002] Plant growth-promoting rhizobacteria (PGPR) refers to microorganisms that live in the rhizosphere and root surface of plants and can directly or indirectly promote or regulate plant growth. Plant rhizosphere growth-promoting bacteria generally have the ability to produce auxin, ammonia, siderophore, antagonism, phosphorus solubilization, salt tolerance, or at least one of them. Inoculation of plant rhizosphere growth-promoting bacteria can make soil nutrients effective, promote plant roots to absorb water and nutrients in the soil, and improve plant growth. Bacillus produces spores that are highly resistant to stress such as heat, ionizing radiation, and certain chemicals. Therefore, the Bacillus inoculum with rhizosphere growth-promoting effect has a simple production process, a long shel...

Claims

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Application Information

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IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C12R1/07
CPCA01N63/00C12N1/20C12N1/205C12R2001/07
Inventor 蔡燕飞曹禺李永涛江亚雄王玉琪王琰张慧敏
Owner SOUTH CHINA AGRI UNIV
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