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Method for preparing antibacterial wound dressing with high chitosan content by means of electrostatic spinning

A technology of wound dressing and chitosan, which is applied in the directions of pharmaceutical formula, rayon manufacturing, textiles and paper making, etc., can solve the problem that it is difficult to obtain continuous chitosan nanofibers, and achieve good antibacterial effect and biocompatibility, Reproducible results with great application potential

Inactive Publication Date: 2016-09-21
DONGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Many researchers have carried out electrospinning by blending chitosan with various materials. However, once the chitosan content is higher than 60%, it is difficult to obtain continuous chitosan nanofibers with good fiber morphology.

Method used

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  • Method for preparing antibacterial wound dressing with high chitosan content by means of electrostatic spinning
  • Method for preparing antibacterial wound dressing with high chitosan content by means of electrostatic spinning
  • Method for preparing antibacterial wound dressing with high chitosan content by means of electrostatic spinning

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] First prepare nano-silver solution, the preparation method of nano-silver solution is:

[0042] A. Distilled water is adjusted to pH 11.5 with NaOH;

[0043] B, preparation mass percentage concentration is the carboxymethyl chitosan solution of 0.2%, measures 10ml solution in transparent glass bottle;

[0044] C. Set the temperature at 70°C, stir at 700rpm, add 1.7M AgNO dropwise 3 until the color of the solution turns yellow;

[0045] D. Take it out after heating and stirring for 1 hour, and keep it away from light for later use.

[0046] The transmission electron microscope image of the prepared nano-silver solution is as follows: figure 1 Shown in (a), the particle size distribution of prepared nano-silver solution is as follows figure 1 (b) shown.

[0047] The preparation method of electrospun antibacterial wound dressing with high chitosan content is as follows:

[0048] (1) Nano-silver solution, dimethyl sulfoxide, acetic acid, and surfactant are made into a m...

Embodiment 2

[0054] (1) Deionized water, dimethyl sulfoxide, acetic acid, and surfactant are formulated into a mixed solvent according to the ratio of 290:35:8:1 for use;

[0055] (2) Take by weighing 0.42g of chitosan and 0.18g of ultra-high molecular weight polyethylene oxide respectively, join in the above-mentioned mixed solvent, place the solution on a heatable magnetic stirrer and stir overnight;

[0056] (3) The next day, the solution was ultrasonically oscillated, and then heated in an oven above 50 degrees for about 15 minutes;

[0057] (4) The electrospinning parameters were set as the spinning voltage was 15kV, the distance between the needle and the receiving plate was 15cm, the propulsion speed of the propulsion pump was 0.15mm / h, and the spinning ambient temperature was 40°C.

[0058] The receiving device is fabricated according to the size of the desired dressing and electrospun. A chitosan dressing without nano-silver with good fiber morphology can be obtained. Figure 4 ...

Embodiment 3

[0060] (1) Deionized water, dimethyl sulfoxide, acetic acid, and surfactant are formulated into a mixed solvent according to the ratio of 290:35:8:1 for use;

[0061] (2) Take the chitosan of 0.30g and the low molecular weight polyethylene oxide of 0.30g respectively, join in the above-mentioned mixed solvent, place the solution on a heatable magnetic stirrer and stir overnight;

[0062] (3) The next day, the solution was ultrasonically oscillated, and then heated in an oven above 50 degrees for about 15 minutes;

[0063] (4) The electrospinning parameters were set as the spinning voltage was 15kV, the distance between the needle and the receiving plate was 15cm, the propulsion speed of the propulsion pump was 0.15mm / h, and the spinning ambient temperature was 40°C.

[0064] The receiving device is fabricated according to the size of the desired dressing and electrospun. Image 6 The scanning electron microscope picture of the dressing shows that a lot of beaded structures ca...

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Abstract

The invention provides a method for preparing antibacterial wound dressing with a high chitosan content by means of electrostatic spinning. The method includes preparing mixed solvents for dissolving chitosan; adding the chitosan and polyethylene oxide with an ultrahigh molecular weight into the mixed solvents and heating and stirring the chitosan, the polyethylene oxide and the mixed solvents to obtain mixed solution; carrying out ultrasonic oscillation on the mixed solution and further heating the mixed solution to obtain spinning solution; preparing the antibacterial wound dressing from the obtained spinning solution by the aid of an electrostatic spinning process. The mixed solvents are prepared from nanometer silver solution, dimethyl sulfoxide, acetic acid and surfactants. The method has the advantages that the method is easy and convenient to implement and good in repeatability, the antibacterial wound dressing prepared by the aid of the method is of a nanometer fiber structure, and proliferation, adhesion, spreading and migration of cells can be induced; excellent antibacterial effects can be realized, the antibacterial wound dressing is excellent in biocompatibility, bionic artificial skins are of structures similar to structures of natural skins, accordingly, infection due to diabetes mellitus can be effectively cured, and the antibacterial wound dressing has huge application potential in diabetes mellitus ulcerative wound treatment.

Description

technical field [0001] The invention relates to a method for preparing an electrospun antibacterial wound dressing with high chitosan content, and belongs to the technical field of tissue engineering and biomanufacturing. Background technique [0002] Diabetic infection is a long-term and difficult-to-cure complication. Because the skin of diabetic patients is easily damaged clinically, the healing after injury is slow, and the wound surface recurs after healing, resulting in excessive inflammation and repair of the wound surface. Poor granulation formation leads to tissue fragility and epithelialization Hysteresis and other pathological manifestations. At the same time, long-term chronic wound infection persists, especially with Pseudomonas aeruginosa infection, local granulation aging, and capsule fibrosis make the wound difficult to heal. It is generally believed that neuropathy, ischemia, and trauma are the three pathogenic factors that make diabetic wounds difficult to...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/28A61L15/18A61L15/26A61L15/44D01F1/10D01F8/16D01F8/18
CPCA61L15/18A61L15/26A61L15/28A61L15/46A61L2300/104A61L2300/232A61L2300/404A61L2400/12D01F1/103D01F8/16D01F8/18C08L5/08C08L71/02
Inventor 莫秀梅周香香于奎朱同贺吴俣王娟穆罕默德阿萨
Owner DONGHUA UNIV
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