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Extraction method of L-citrulline in seed melon, and preparation method of effervescent tablet

An extraction method and citrulline technology, which are applied in the preparation of organic compounds, the preparation of urea derivatives, chemical instruments and methods, etc., can solve the problems of high residues of other amino acids, low product purity, difficult process control, etc. The agent is smooth and tidy, the process flow is short, and the effect of improving product purity

Inactive Publication Date: 2016-09-07
SHIHEZI DEV ZONE SHIDA HEXU TECH DEV CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, the methods for producing L-citrulline are mainly fermentation, enzymatic, synthetic and extraction methods. The chemical method or biosynthetic method used in the prior art has cumbersome steps and high cost, and there are complex processes such as enzymatic conversion. This process is relatively difficult to control, and often contains a large amount of D-citrulline, the optical antipode; while the existing extraction methods include pressing, concentration, cation exchange, crystallization and other steps, these methods all have other amino acid residues that are too high and the product purity Not high, producing a lot of sewage and other disadvantages

Method used

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  • Extraction method of L-citrulline in seed melon, and preparation method of effervescent tablet
  • Extraction method of L-citrulline in seed melon, and preparation method of effervescent tablet
  • Extraction method of L-citrulline in seed melon, and preparation method of effervescent tablet

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preparation example Construction

[0059] The present invention also provides a preparation method of L-citrulline effervescent tablet in seed melon, the method firstly provides a composition for effervescent tablet, and the composition includes the following components by weight:

[0060] 25-35 parts of L-citrulline, 17-21 parts of citric acid, 10-14 parts of mannitol, 2-4 parts of sucralose, 0.1-0.5 parts of steviol glycoside, PVP-k 30 4-6 parts of ethanol A solution;

[0061] 14-17 parts of sodium bicarbonate, 16-20 parts of mannitol, PVP-k 30 5-9 parts of ethanol B solution;

[0062] 0.6-1.0 parts of lemon essence, 1.8-2.5 parts of polyethylene glycol 6000;

[0063] L-citrulline is prepared according to the aforementioned extraction method of L-citrulline in seed melon, PVP-k 30 Ethanol A solution is PVP-k with a mass concentration of 90% 30 Ethanol solution, PVP-k 30 Ethanol B solution is the PVP-k that mass concentration is 20% 30 weak.

[0064] Commonly used fillers for preparing effervescent tabl...

Embodiment 1

[0084] A method for extracting L-citrulline in seed melon, comprising the steps of:

[0085] 1) Preparation of ultrafiltrate: squeeze the juice from the seeded melon pulp, centrifuge, and take the supernatant, vacuum filter the supernatant with a flat membrane with a pore size of 0.2um, and select the ultrafiltrate with a molecular weight cut-off of 5000 Daltons. Flat membrane ultrafiltration for 50 minutes, and then vacuum concentration until the mass concentration of L-citrulline was 4.0 mg / mL to obtain the ultrafiltrate;

[0086] 2) Elution on the column: adjust the pH value of the ultrafiltrate to 1.2 to obtain the upper column liquid, pass the upper column liquid into the 732 type cation exchange resin with a loading capacity of 20ml to exchange and adsorb citrulline, and the flow rate through the column is 7.2BV / h, when the mass concentration of L-citrulline in the effluent is 4.0mg / mL, the adsorption is complete, and the ammonia water with a molar concentration of 0.6m...

Embodiment 2

[0096] A method for extracting L-citrulline in seed melon, comprising the steps of:

[0097] 1) Preparation of ultrafiltrate: squeeze the juice from the seeded melon pulp, centrifuge, and take the supernatant, vacuum filter the supernatant with a flat membrane with a pore size of 0.1um, and select the ultrafiltrate with a molecular weight cut-off of 7000 Daltons. Flat membrane ultrafiltration for 20 minutes, and then vacuum concentration until the mass concentration of L-citrulline was 3.0 mg / mL to obtain the ultrafiltrate;

[0098] 2) Elution on the column: adjust the pH value of the ultrafiltrate to 2.0 to obtain the upper column liquid, pass the upper column liquid into the 001×7 type cation exchange resin with a loading capacity of 25ml to exchange and adsorb citrulline, and the flow rate through the column is 3.6 BV / h, the adsorption is complete when the mass concentration of L-citrulline in the effluent is 3.0mg / mL, and the ammonia water with a molar concentration of 0.9...

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Abstract

The invention discloses an extraction method of L-citrulline in seed melon. The method comprises the steps of ultrafiltration liquid preparation, column elution, reduced-pressure distillation and crystallization. The invention also discloses an application of L-citrulline extracted with the above method. Specifically, materials with needed weight are prepared into acid particles and alkali particles; the particles are dried, and a lemon essence is sprayed in; the particles are dried, and are well mixed with polyethylene glycol 6000; and the mixture is tableted, such that L-citrulline effervescent tablets are obtained. The extraction method provided by the invention has the advantages of simple preparation process, low cost, low energy consumption, and low waste and pollutant production. The effervescent tablets prepared with the extracted L-citrulline have the advantages of fast disintegration, good effervescent effect, good sweet and sour taste, and convenient application. With the methods provided by the invention, a novel approach for comprehensively developing and utilizing seed melons is provided.

Description

technical field [0001] The invention belongs to the field of agricultural product processing, and relates to a method for extracting L-citrulline from seed melon and a method for preparing effervescent tablets. Background technique [0002] Seed melon is a branch of watermelon. Compared with the common edible watermelon, seed melon is mainly planted to obtain melon seeds. The pulp and rind of the melon are discarded as waste. The waste of resources is serious and farmers suffer huge economic losses. , It also greatly hinders the development of the seed melon industry, so comprehensive development and utilization of the seed melon is an inevitable choice. [0003] L-citrulline in seed melon is a non-essential amino acid. In the prevention and treatment of prostate diseases, L-citrulline has obvious effects. Unlike drugs that improve sexual performance, L-citrulline can improve male sexual function and can be completely absorbed into the blood. In the human body, since L-cit...

Claims

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Application Information

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IPC IPC(8): C07C273/18C07C275/16A61K9/46A61K47/34A61K31/198
CPCA61K9/0007A61K9/2031A61K9/2095A61K31/198C07C273/189C07C275/16
Inventor 陈文王军
Owner SHIHEZI DEV ZONE SHIDA HEXU TECH DEV CO LTD
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