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Method for improving thermal stability of collagen through ultrahigh pressure treatment

An ultra-high pressure, thermally stable technology that can be used in the field of medical biomaterials to solve problems such as reduced biosafety

Inactive Publication Date: 2016-09-07
WUHAN POLYTECHNIC UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this chemical treatment method needs to introduce new chemical substances in the natural collagen, and the biosafety of the product is significantly reduced

Method used

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  • Method for improving thermal stability of collagen through ultrahigh pressure treatment
  • Method for improving thermal stability of collagen through ultrahigh pressure treatment
  • Method for improving thermal stability of collagen through ultrahigh pressure treatment

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Bullfrog skin was selected as raw material, natural collagen was extracted by acetic acid combined with pepsin, the extract was purified by salting out, dialysis and other methods, and then freeze-dried to obtain bullfrog enzyme-soluble collagen products. The collagen was analyzed by SDS-PAGE and circular dichroism (CD), and it was confirmed that it was a natural type I collagen with a complete triple helix molecular structure. Under the condition of ice bath, the collagen was dissolved with acetic acid aqueous solution (to obtain a collagen solution with a concentration of 3 mg / mL), and then the collagen solution was placed in a polyethylene bag, which was vacuum-sealed and packaged by a vacuum packaging machine. The packaging bag is placed in an ultra-high pressure container, and water is used as a pressure transmission liquid medium for ultra-high pressure treatment. The treatment conditions are as follows: the pressure is 300MPa, the time for the pressure holding tr...

Embodiment 2

[0027] Bullfrog skin was selected as raw material, natural collagen was extracted by acetic acid combined with pepsin, the extract was purified by salting out, dialysis and other methods, and then freeze-dried to obtain bullfrog enzyme-soluble collagen products. The collagen was analyzed by SDS-PAGE and circular dichroism (CD), and it was confirmed that it was a natural type I collagen with a complete triple helix molecular structure. Under the condition of ice bath, the collagen was dissolved with acetic acid aqueous solution (to obtain a bullfrog skin collagen solution with a concentration of 2 mg / mL), and then the bullfrog skin collagen solution was placed in a polypropylene bag, which was vacuum-sealed and packaged by a vacuum packaging machine. The packaging bag is placed in an ultra-high pressure container, and water is used as a pressure transmission liquid medium for ultra-high pressure treatment. The treatment conditions are as follows: the pressure is 200MPa, the tim...

Embodiment 3

[0029] Grass carp skin was selected as raw material, and natural collagen was extracted by using acetic acid as a solvent. The extract was purified by salting out, dialysis and other methods, and then freeze-dried to obtain grass carp acid-soluble collagen products. The collagen was analyzed by SDS-PAGE and circular dichroism (CD), and it was confirmed that it was a natural type I collagen with a complete triple helix molecular structure. Under the condition of ice bath, the collagen was dissolved with acetic acid aqueous solution (to obtain a grass carp skin collagen solution with a concentration of 3 mg / mL), and then the grass carp skin collagen solution was placed in a polyethylene bag, which was vacuum sealed and packaged by a vacuum packaging machine. The packaging bag is placed in an ultra-high pressure container, and water is used as a pressure transmission liquid medium for ultra-high pressure treatment. The treatment conditions are as follows: the pressure is 300MPa, ...

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Abstract

The invention discloses a method for improving the thermal stability of collagen through ultrahigh pressure treatment. The method comprises the following steps of (1) dissolving natural collagen with an acetic acid aqueous solution so as to obtain a collagen solution; (2) placing the collagen solution in a soft packaging material, and performing vacuumized and sealed packaging; (3) placing the soft packaging material after sealed packaging in an ultrahigh pressure vessel, and performing ultrahigh pressure treatment; and (4) taking out the collagen solution, and performing freeze drying so as to obtain a collagen product of which the thermal stability is improved. According to the method disclosed by the invention, through an ultrahigh pressure physical treatment method, the thermal stability of collagen molecules is effectively improved. Compared with conventional methods of chemical modification, chemical crosslinking and the like, the method disclosed by the invention has the advantages that the basic structural composition of the collagen molecules is not changed, new chemical components are not introduced, the unique bioactivity and the biological safety of the collagen are not changed, and the treatment means are simple and practicable to implement.

Description

technical field [0001] The invention belongs to the technical field of medical biomaterials, and in particular relates to a method for improving thermal stability of collagen by using ultra-high pressure treatment. Background technique [0002] Collagen is a class of structural proteins that widely exist in multicellular organisms. Its typical feature is that the protein molecule is composed of three peptide chains, and there is a superhelical molecular structure (collagen domain) formed by the winding of the three peptide chains. Due to the excellent biocompatibility, degradability and good mechanical properties of natural collagen, it has been widely used in the fields of biomedical materials, tissue engineering and medical cosmetology. [0003] Maintaining the complete triple helix molecular structure is the structural basis for natural collagen to maintain its biological function and biological activity. After heat treatment, the triple helix structure of natural collag...

Claims

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Application Information

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IPC IPC(8): A23L3/015
CPCA23L3/0155A23V2002/00A23V2300/46
Inventor 汪海波汪海婴
Owner WUHAN POLYTECHNIC UNIVERSITY
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