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Ratio fluorescence sensor and visualized detection method for glucose

A technology of ratiometric fluorescence and ratiometric fluorescent probes, which is applied in the field of visual detection of glucose content with dual-emission fluorescent probes, can solve complex pre-processing and large-scale scientific instruments, limit real-time on-site detection of blood sugar content, and difficult to achieve quantitative detection of glucose, etc. , to achieve intuitive and obvious color changes, broaden the application range, and simple preparation methods

Inactive Publication Date: 2016-08-31
HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the detection of glucose is mainly realized by electrochemical method and spectrophotometry. However, these two methods are easily affected by substances such as proteins in the blood, and require complex pre-treatment and large-scale scientific instruments. The detection cost is high, which limits Real-time on-site detection and daily monitoring of blood sugar content
In recent years, analytical methods for glucose detection using colorimetry and paper microfluidics have emerged. However, these methods have low detection sensitivity due to inconspicuous color changes, and it is also difficult to achieve quantitative detection of glucose.

Method used

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  • Ratio fluorescence sensor and visualized detection method for glucose

Examples

Experimental program
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Effect test

Embodiment 1

[0041] 1. Preparation of quantum dots

[0042] 0.2283g cadmium chloride (CdCl 2 2.5H 2O) was added to 100 mL of deoxygenated ultrapure water, followed by adding 209 μL of mercaptopropionic acid, and then adjusted the pH to 9 with 1M NaOH to form a mixed solution; take 0.0319g tellurium powder and 0.05g sodium borohydride in 2mL ultrapure water , reacted in ice bath for more than 8h under nitrogen protection to generate sodium tellurium hydride, put 5mL of 0.5M H 2 SO 4 The solution is injected into the resulting sodium tellurium hydride solution, and the resulting H 2 All Te was passed into the mixed solution, heated to reflux after stirring for 20 minutes, and the reflux reaction time was controlled to obtain an aqueous solution of cadmium telluride quantum dots with mercaptopropionic acid-stabilized fluorescence emission peaks at 490-680 nm. The prepared aqueous solution of cadmium telluride quantum dots was irradiated under a 15W ultraviolet lamp to improve the fluoresc...

Embodiment 2

[0054] 1. Preparation of quantum dots

[0055] The preparation process of this step is the same as that in Example 1.

[0056] 2. Preparation of Quantum Dot-Embedded Silica Nanoparticles

[0057] The preparation process of this step is the same as that in Example 1.

[0058] 3. Preparation of carbon dots

[0059] Dissolve 1.0507g of citric acid and 335μL of ethylenediamine in 20mL of ultrapure water, transfer this mixed solution to a 30mL polytetrafluoroethylene reactor, react at 200° C. for 5 hours, and dialyze the obtained carbon dots for 24 hours, for later use. ;

[0060] 4. Preparation of dual emission ratiometric fluorescent probes

[0061] 0.05mg / mL carbon dot solution, 2mg / mL condensing agent 1-(3-dimethylaminopropyl)-3-ethylcarbodiamine and N-hydroxysuccinimide (mass ratio 1: 1) Mix with ultrapure water in a volume ratio of 1:10:30 in the dark, add 10 mg of quantum dot-embedded silica nanoparticles after stirring for 30 minutes, and stir at room temperature for 1...

Embodiment 3

[0068] 1. Preparation of quantum dots

[0069] The preparation process of this step is the same as that in Example 1.

[0070] 2. Preparation of Quantum Dot-Embedded Silica Nanoparticles

[0071] The preparation process of this step is the same as that in Example 1.

[0072] 3. Preparation of carbon dots

[0073] Dissolve 1.0507g of citric acid and 335μL of ethylenediamine in 20mL of ultrapure water, transfer this mixed solution to a 30mL polytetrafluoroethylene reactor, react at 200° C. for 5 hours, and dialyze the obtained carbon dots for 24 hours, for later use. ;

[0074] 4. Preparation of dual emission ratiometric fluorescent probes

[0075] 0.05mg / mL carbon dot solution, 2mg / mL condensing agent 1-(3-dimethylaminopropyl)-3-ethylcarbodiamine and N-hydroxysuccinimide (mass ratio 1: 1) Mix with ultrapure water in a volume ratio of 1:10:30 in the dark, add 10 mg of quantum dot-embedded silica nanoparticles after stirring for 30 minutes, and stir at room temperature for 1...

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Abstract

The invention discloses a ratio fluorescence sensor and a visualized detection method for glucose. The ratio fluorescence sensor is prepared by printing uniformly distributed ratio fluorescence probes on a paper medium in an ink-jet printing manner with dual-emitting ratio fluorescence probes as ink. The visualized detection method for glucose in virtue of the ratio fluorescence sensor comprises the following steps: adding glucose oxidase into a glucose solution, carrying out a reaction at 37 DEG C for 40 min, then adding an excess ferrous solution and dripping the solution onto a paper sensor so as to realize visualized detection of glucose. The dual-emitting fluorescent chemical sensor used for visualized detection of glucose is designed and developed by using carbon points and quantum dots capable of emitting light under excitation of a light source with a single wavelength; and the sensor has the characteristics of high selectivity, high sensitivity, visual detection results and capacity of realizing quantitative detection.

Description

1. Technical field [0001] The invention relates to a ratio fluorescent sensor and a method for visually detecting glucose, which is a method for visually detecting glucose content with a dual-emission fluorescent probe prepared by an inkjet printer. 2. Background technology [0002] The analysis and detection of glucose is of great significance to the diagnosis, treatment and control of human health and diseases. At present, the detection of glucose is mainly realized by electrochemical method and spectrophotometry. However, these two methods are easily affected by substances such as proteins in the blood, and require complex pre-treatment and large-scale scientific instruments. The detection cost is high, which limits Real-time on-site detection and daily monitoring of blood sugar levels. In recent years, analytical methods for glucose detection using colorimetry and paper microfluidics have emerged. However, these methods have low detection sensitivity due to inconspicuou...

Claims

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Application Information

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IPC IPC(8): G01N21/64G01N21/78
CPCG01N21/6428G01N21/78
Inventor 张忠平都述虎黄晓燕蒋长龙刘变化刘仁勇
Owner HEFEI INSTITUTES OF PHYSICAL SCIENCE - CHINESE ACAD OF SCI
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