Canine distemper/infectious hepatitis/parvovirus triple egg yolk antibody and preparation method thereof

A canine parvovirus and canine distemper virus technology, applied in the direction of antibodies, antiviral agents, chemical instruments and methods, can solve the problems of serious environmental pollution and influence on antibody activity, achieve stable physical and chemical properties, reduce interference, improve The effect of accuracy

Inactive Publication Date: 2016-08-24
内蒙古神元生物工程股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method removes lipids more thoroughly, the supernatant is clear and transparent, and the antibody recovery rate is high, but the residual organic solvent in the supernatant has an impact on the activity of the antibody, and the environmental pollution caused by organic solvents is relatively serious, so this method is only suitable for experiments small volume preparation

Method used

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  • Canine distemper/infectious hepatitis/parvovirus triple egg yolk antibody and preparation method thereof
  • Canine distemper/infectious hepatitis/parvovirus triple egg yolk antibody and preparation method thereof
  • Canine distemper/infectious hepatitis/parvovirus triple egg yolk antibody and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] 1 immunity of laying hens

[0047] Raise the purchased 35-week-old Isa laying hens for 7-10 days first, with the same length of feed and light as before the purchase, so that they can adapt to the new environment and reduce stress.

[0048] The 120 laying hens were randomly divided into 2 groups: 40 in group A (test group 1), 40 in group B (test 2), and 40 in group C (control group).

[0049] Group A: Chickens of group A were immunized with a multivalent vaccine containing canine distemper-canine infectious hepatitis-canine parvovirus disease, 1.5 birds per bird. After immunization, eggs were collected continuously for 2 weeks and the antibody titer was determined. Whether the antibody titer reaches the required level (1:512), if the antibody titer does not meet the required level, boost the immunization, collect eggs, measure the antibody titer, and determine the number of immunizations until the required level of titer is reached.

[0050] Group B: Chickens in group B were i...

Embodiment 2

[0055] Preparation of Canine Distemper-Canine Infectious Hepatitis-Canine Parvovirus Disease 3 Combined Yolk Antibody Injection

[0056] First, add maltose (90-100g / L) as a stabilizer, thimerosal (0.003%~0101%) as a preservative, and use water for injection in the physiological saline solution that has prepared the antibody titer and reaches a certain standard for egg-yolk antibody. After configuration, through coarse filtration, fine filtration, filling, sterilization, light inspection, labeling, packaging, and storage, the finished product of canine distemper-canine infectious hepatitis-canine parvovirus disease 3-linked egg yolk antibody injection is obtained.

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Abstract

The invention provides a preparation method of a canine distemper / infectious hepatitis / parvovirus triple egg yolk antibody and provides the canine distemper / infectious hepatitis / parvovirus triple egg yolk antibody prepared with the preparation method. The preparation method comprises steps as follows: eggs laid by immunized layer hens are collected and subjected to surface sterilization, egg yolk is separated, homogenized and diluted with sterile distilled water, pH is regulated to 5.0-5.2, a mixture is left to stand, and a supernatant is collected; the supernatant is diluted with an acetate buffer solution, pH is regulated to 5.0-5.2, n-caprylic acid is added, a mixture is left to stand for layering, a water phase is taken and centrifuged, precipitates are taken and dissolved in normal saline, filtering is performed, and the egg yolk antibody is obtained. The egg yolk antibody has the advantages of stable physicochemical properties, wide sources, high output and low cost as well as large animal phylogenetic distance, is more suitable for producing specific antibodies, can be used for preparing a diagnostic reagent, controlling diseases and serving as a feed additive, and has the potential in development of functional foods and new drugs.

Description

Technical field [0001] The invention relates to the field of biology, in particular to a canine distemper-infectious hepatitis-parvovirus triple egg yolk antibody and a preparation method thereof. Background technique [0002] Canine distemper virus, canine infectious hepatitis virus and canine parvovirus disease are common canine viral infectious diseases all over the world. Although various countries have increased the immune prevention and control of these three diseases, due to this The three diseases are characterized by high contact and lethality, so that these three diseases are often prevalent all over the world and caused huge economic losses. Canine distemper is caused by canine distemper virus (CDV), and its main clinical symptoms are: "biphasic fever" changes in body temperature, pneumonia, runny nose, and eye drops; canine infectious hepatitis is caused by dogs Canineadenovirus virus type 1 (CAV-l) is also known as Canine infectious hepatitis virus (ICHV). The main ...

Claims

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Application Information

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IPC IPC(8): C07K16/10C07K16/08C07K16/02A61K39/42A61P31/20A61P31/14
CPCC07K16/02C07K16/081C07K16/1027
Inventor 郑润宽付改玲高永刚刘永清王娟梁伟闵长安
Owner 内蒙古神元生物工程股份有限公司
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