Preparation method for producing pig haemocyte active small peptide powder through one-step method

Abstract

The invention relates to a preparation method for producing pig haemocyte active small peptide powder through a one-step method. The preparation method mainly comprises the steps of collecting fresh pig blood subjected to anticoagulation treatment, conducting centrifugal separation to obtain haemocyte liquid, conducting hemolysis, enzymolysis and centrifugation to obtain enzymatic hydrolysate, conducting ultrafiltration membrane filtration and nanofiltration membrane concentration on the enzymatic hydrolysate, and then conducting spray drying, so that the pig haemocyte active small peptide powder is obtained. According to the preparation method, efficient endo protease is selected, the one-step method is adopted, the ultrafiltration membrane and nanofiltration membrane separation technology is combined, the process procedure is simple, the production cycle is short, production efficiency is high, cost is low, and the preparation method is suitable for large-scale industrialized production. In the prepared pig haemocyte active small peptide powder, the content of active small peptide (with the relative molecular weight ranging from 180 Da to 1000 Da) reaches 75% or above, the content of organic hemin (heme iron) reaches 0.2% or above, the content of ash is lower than 5.0%, and the content of free amino acid is lower than 11%. The product can promote growth of animals and enhance immunity and disease resistance of animals and is a novel functional protein feed raw material.

Description

technical field [0001] The invention relates to the technical field of deep processing of animal blood protein, in particular to a preparation method for producing porcine blood cell active small peptide powder by one-step enzymatic hydrolysis. Background technique [0002] According to the number of amino acid residues, peptides can be divided into polypeptides, oligopeptides, and small peptides. Generally speaking, those containing more than 50 amino acid residues are usually called proteins, those with less than 50 and more than 10 amino acid residues are called polypeptides, and those with less than 10 amino acid residues are called oligopeptides. Small peptides refer to dipeptides, tripeptides and tetrapeptides with a relative molecular weight between 180 and 1000 Da. A large number of studies have shown that there is a small peptide absorption mechanism in animals, and small peptides can enter the body circulation through intestinal mucosal cells intact. Animals abso...

AI Technical Summary

Problems solved by technology

However, this technical method requires two-step enzymatic hydrolysis treatment, especially in the acidic protease hydrolysis process, under acidic conditions (PH2-3) part of hemoglobin is prone to denaturation, and precipitates together with heme, and is removed by centrifugation. The recovery rate of protein is low, especially the loss of organic porphyrin iron (heme iron), which is an important source of iron supplementation for animals. At the same time, due to the repeated adjustment of the pH value, more salt is brought in, resulting in a relatively low ash content in the prepared product. High, the content of active small molecule peptides (relative molecular weight between 180 and 1000Da) in the product has not been reported

CN201410547593.3 discloses a preparation method of porcine blood globulin powder, using whole porcine blood as raw material, preparing porcine blood hemoglobin liquid through centrifugation and ultrasonic treatment, and then performing physical deoxygenation, ultrasonic treatment, and compound enzyme separation on the hemoglobin liquid in sequence Stage enzymatic hydrolysis, activated carbon decolorization, membrane ultrafiltration concentration, spray drying and other complex process processes, and finally milky white porcine hemoglobin polypeptide powder and heme peptide, although this method completely removes the color of hemoglobin polypeptide powder And bloody smell, the sensory properties of the product have been greatly improved, and the digestion and absorption rate of protein has been improved, but it requires multiple ultrasonic treatments, segmental enzymatic hydrolysis, repeated centrifugation, and decolorization and concentration. The process is cumbersome and the cost is high. Protein peptide powder, does not contain active small molecular peptides, and the economic value is not high

Therefore, the prior art solutions have deficiencies both in the ease and operability of the preparation process and in the nutritional value and functionality of the product.

Images