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Method for evaluating immune effect of infectious bursal disease virus vaccine

A technology of immune effect and evaluation method, which is applied in the fields of pharmaceutical formulations, preparations for in vivo experiments, compound screening/testing, etc., and can solve problems such as unsatisfactory effects

Inactive Publication Date: 2016-08-17
兆丰华生物科技(福州)有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the current standard, live vaccines are tested for virus potency by measuring the virus content, and inactivated vaccines are tested for eligibility by neutralizing antibody tests. Using these two methods to test whether the vaccine is qualified or even has a high potency is not effective in clinical use. not ideal

Method used

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  • Method for evaluating immune effect of infectious bursal disease virus vaccine
  • Method for evaluating immune effect of infectious bursal disease virus vaccine
  • Method for evaluating immune effect of infectious bursal disease virus vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] A method for evaluating the immune effect of a bursal vaccine comprising the following steps:

[0047] 1) Prepare 30 7-day-old chickens as experimental chickens, and divide the experimental chickens into a live vaccine group, an inactivated vaccine group and a control group, each with 10 chickens;

[0048] 2) immunize experimental chickens with the bursal vaccine to be detected, and the bursal vaccine includes live bursal vaccine and inactivated bursal vaccine;

[0049] Live vaccine group: immunize the chickens of the live vaccine group with the live vaccine of the Bursal of Fabricius, and the immunization method is in accordance with the instructions for use of the live vaccine of the Bursal of Fabricius;

[0050] Inactivated vaccine group: immunize the chickens of the inactivated vaccine group with the inactivated vaccine of the bursa to be detected, and the immunization method is in accordance with the instructions for use of the inactivated vaccine of the bursa;

...

Embodiment 2

[0063] A method for evaluating the immune effect of a bursal vaccine comprising the following steps:

[0064] 1) Prepare 30 30-day-old chickens as experimental chickens, and divide the experimental chickens into a live vaccine group, an inactivated vaccine group and a control group, each with 10 chickens;

[0065] 2) immunize experimental chickens with the bursal vaccine to be detected, and the bursal vaccine includes live bursal vaccine and inactivated bursal vaccine;

[0066] Live vaccine group: immunize the chickens of the live vaccine group with the live vaccine of the Bursal of Fabricius, and the immunization method is in accordance with the instructions for use of the live vaccine of the Bursal of Fabricius;

[0067] Inactivated vaccine group: immunize the chickens of the inactivated vaccine group with the inactivated vaccine of the bursa to be detected, and the immunization method is in accordance with the instructions for use of the inactivated vaccine of the bursa;

...

Embodiment 3

[0080] A method for evaluating the immune effect of a bursal vaccine comprising the following steps:

[0081] 1) 45 15-day-old chickens were prepared as experimental chickens, and the experimental chickens were divided into a live vaccine group, an inactivated vaccine group and a control group, each with 15 chickens;

[0082] 2) immunize experimental chickens with the bursal vaccine to be detected, and the bursal vaccine includes live bursal vaccine and inactivated bursal vaccine;

[0083] Live vaccine group: immunize the chickens in the live vaccine group with the bursal live vaccine. The immunization method is in accordance with the instructions for use of the bursal live vaccine, and each chicken is orally 1 part of the bursal live vaccine;

[0084] Inactivated vaccine group: immunize the chickens in the inactivated vaccine group with the inactivated vaccine of the bursa to be tested. The immunization method is in accordance with the instructions for use of the inactivated ...

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Abstract

The invention discloses a method for evaluating the immune effect of an infectious bursal disease virus vaccine, which comprises the following steps: respectively immunizing chickens of a live vaccine group and an inactivated vaccine group by an infectious bursal disease virus live vaccine and an infectious bursal disease virus inactivated vaccine; enabling all chickens to orally take the infectious bursal disease virus strain BC6-85 for counteracting toxic substances for 72 hours; then dissecting the bursa of Fabricius; and observing whether the bursa of Fabricius has a lesion, thereby evaluating the immune effect of the vaccine. According to practical demands, the varieties, ages in days and used data of the selected chickens are consistent, so that the immune effect of the vaccine in a practical use object can be more accurately evaluated.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for evaluating the immune effect of a bursal vaccine. Background technique [0002] Chicken infectious bursal disease is an acute and contagious disease of chickens caused by Reovirus. Due to the sudden onset of the disease, short course of disease, high mortality, and the immunosuppression of chickens, it is still one of the main infectious diseases in the chicken industry. [0003] There are two types of vaccines for bursal virus on the market: live vaccines and inactivated vaccines. Live vaccines with weak virulence have poor immune effect, and those with strong virulence can damage the bursa of Fabricius and affect the immune effect; inactivated vaccines retain the complete virus structure and greatly improve the safety performance without affecting the immune effect. The effect of using small day-old chickens is limited. How to accurately evaluate the imm...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K49/00
CPCA61K49/0008
Inventor 赵华娥
Owner 兆丰华生物科技(福州)有限公司
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