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Preparation method for preparing inactivated vaccine by means of Muscovy duck hepatitis virus

A Muscovy duck hepatitis and inactivated vaccine technology, which is applied in the direction of antiviral agents, medical preparations containing active ingredients, pharmaceutical formulas, etc., can solve the problem of stunted development of Muscovy ducks, affecting the economic benefits of Muscovy ducks, and low cure rate, etc. problems, to achieve the effects of stimulating the development of the body's immune cells, enhancing the body's immune function, and quickly absorbing the vaccine

Inactive Publication Date: 2016-08-17
CHONGQING SANJIE ZHONGXIN BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After clinical onset, conventional antiviral combined with antibacterial treatment plan, the effect is not good, the cure rate is less than 40%, the course of disease is long, the development of resistant Muscovy duck is hindered, the feed meat is more than 20% higher than that of normal Muscovy duck, and the time for slaughter is extended by 15 days Above, the development is irregular, which has seriously affected the economic benefits of Muscovy duck breeding

Method used

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  • Preparation method for preparing inactivated vaccine by means of Muscovy duck hepatitis virus
  • Preparation method for preparing inactivated vaccine by means of Muscovy duck hepatitis virus
  • Preparation method for preparing inactivated vaccine by means of Muscovy duck hepatitis virus

Examples

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Effect test

Embodiment 1

[0023] A preparation method of muscovy duck hepatitis virus recombinant chicken colony-stimulating factor adjuvant inactivated vaccine, which uses muscovy duck hepatitis virus GS14 strain as an immunogen and recombinant chicken colony-stimulating factor as an adjuvant, and its specific steps are as follows:

[0024] (1) Antigen preparation: Dilute Muscovy duck hepatitis virus GS14 strain 10,000 times with normal saline, inoculate 11-12-day-old susceptible Muscovy duck embryos through the allantoic cavity, 0.2ml / embryo, collect and inoculate and die within 60-168 hours Muscovy duck embryo allantoic fluid, if the sterility test is qualified, is the antigen, and then stored at -40°C;

[0025] (2) Antigen inactivation: the antigen in step (1) is added into a formaldehyde solution with a final volume concentration of 0.1%, and inactivated at 37°C for 24 hours, during which the antigen is shaken 4 times for 3 minutes each time; the formaldehyde solution The content of formaldehyde i...

Embodiment 2

[0028] A preparation method of muscovy duck hepatitis virus recombinant chicken colony-stimulating factor adjuvant inactivated vaccine, which uses muscovy duck hepatitis virus GS14 strain as an immunogen and recombinant chicken colony-stimulating factor as an adjuvant, and its specific steps are as follows:

[0029] (1) Antigen preparation: Dilute Muscovy duck hepatitis virus GS14 strain 5000 times with normal saline, inoculate 11-12 day-old susceptible Muscovy duck embryos through the allantoic cavity, 0.1ml / embryo, collect and inoculate and die within 60-168 hours Muscovy duck embryo allantoic fluid, if the sterility test is qualified, is the antigen, and then stored at -20°C;

[0030] (2) Antigen inactivation: the antigen in step (1) is added into a formaldehyde solution with a final volume concentration of 0.1%, and inactivated at 37°C for 24 hours, during which the antigen is shaken 4 times for 3 minutes each time; the formaldehyde solution The content of formaldehyde in ...

Embodiment 3

[0033] A preparation method of muscovy duck hepatitis virus recombinant chicken colony-stimulating factor adjuvant inactivated vaccine, which uses muscovy duck hepatitis virus GS14 strain as an immunogen and recombinant chicken colony-stimulating factor as an adjuvant, and its specific steps are as follows:

[0034] (1) Antigen preparation: Dilute Muscovy duck hepatitis virus GS14 strain 8000 times with normal saline, inoculate 12-day-old susceptible Muscovy duck embryos through the allantoic cavity, 0.15ml / embryo, collect and inoculate dead Muscovy ducks within 60-168 hours Embryo allantoic fluid, if the sterility test is qualified, is the antigen, and then stored at -30°C;

[0035] (2) Antigen inactivation: the antigen in step (1) is added into a formaldehyde solution with a final volume concentration of 0.1%, and inactivated at 37°C for 24 hours, during which the antigen is shaken 4 times for 3 minutes each time; the formaldehyde solution The content of formaldehyde in the ...

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Abstract

The invention provides a preparation method for preparing a Muscovy duck hepatitis virus recombinant chicken colony stimulating factor adjuvant inactivated vaccine by means of a Muscovy duck hepatitis virus GS14. The vaccine is prepared by taking the Muscovy duck hepatitis virus GS14 as immunogen and taking a recombinant chicken colony stimulating factor as an adjuvant. The Muscovy duck hepatitis virus GS14 is preserved in China Center for Type Culture Collection on April 27, 2016, and the preservation number is CCTCC No:V201623. The inactivated vaccine has the advantages that the vaccine can be intramuscularly injected or subcutaneously injected in use and can be rapidly absorbed, no granuloma is formed, a stress reaction to young Muscovy ducks basically does not exist, and the vaccine has better safety compared with propolis and oil adjuvant vaccines. When the vaccine is used for prevention, the protection rate is 90% or above, and the protection period lasts for three months; the recombinant chicken colony stimulating factor, serving as the immunologic adjuvant, in the vaccine has the effect of stimulating immune cell development of organisms and can non-specifically enhance the immune function of the organisms.

Description

technical field [0001] The invention relates to a preparation method of a vaccine, in particular to a preparation method of a muscovy duck hepatitis virus recombinant chicken colony-stimulating factor adjuvant inactivated vaccine using the muscovy duck hepatitis virus GS14 strain. Background technique [0002] Since 2013, Muscovy ducks have been prevalent in some areas of my country. The clinical symptoms are a sharp increase in feed intake and then a basic hunger strike. They are depressed, like to lie down, have yellow-green loose stools, and occasionally have respiratory rales. The sick duck is dehydrated and emaciated. Before death, there were neurological symptoms similar to duck viral hepatitis; autopsy symptoms were occasional pericardial effusion, myocardial hemorrhage and pancreatic necrosis, common liver enlargement, at least 20% larger than the normal Muscovy duck liver of the same age, and the background color of the whole liver It is dark yellow or off-white inste...

Claims

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Application Information

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IPC IPC(8): A61K39/125A61K39/39A61P31/14
Inventor 魏思远赵光伟
Owner CHONGQING SANJIE ZHONGXIN BIOLOGICAL ENG CO LTD
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