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Bdellovibrio bacteriovorus bacterial strain eliminating aquatic product Gram-positive pathogenic bacterium and application thereof

A gram-positive, leech vibrio technology, applied in applications, bacteria, disinfectants, etc., to ensure high quality, safety effects, and significant application effects

Inactive Publication Date: 2010-02-17
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, some microecological preparations still have many defects in the prevention and treatment of aquatic animal diseases, and Bdellovibrio bacteriophage has unique biological characteristics of lysing bacteria, which has opened up a new way for the prevention and treatment of aquatic animal diseases. A green, Environmentally friendly and healthy natural "antibiotics" are gradually entering the aquaculture industry

Method used

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  • Bdellovibrio bacteriovorus bacterial strain eliminating aquatic product Gram-positive pathogenic bacterium and application thereof
  • Bdellovibrio bacteriovorus bacterial strain eliminating aquatic product Gram-positive pathogenic bacterium and application thereof
  • Bdellovibrio bacteriovorus bacterial strain eliminating aquatic product Gram-positive pathogenic bacterium and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] (1) Isolation and purification of Bdellovibrio BDS02

[0049] The host Aeromonas hydrophila (Aeromonas hydrophila, purchased from the Culture Collection Center of Guangdong Institute of Microbiology, No. GIM1.172) was inoculated in 100 mL of sterilized LB (Luria-Bertani) liquid medium (10 g of peptone, 5 g of yeast extract) , distilled water 1000mL, pH 7.2) in a conical flask, placed in a constant temperature shaker with a rotation speed of 200rpm and a temperature of 28°C for 16h, so that it was in the logarithmic growth phase. The culture solution was centrifuged at 6000rpm for 20min at 4°C, the supernatant was discarded, and the precipitated cells were mixed with 1mL DNB (dilute nutrient broth) liquid medium (0.8g nutrient broth, 0.5g casein acid hydrolyzate g, yeast extract 0.1g, dissolved in 1000mL distilled water, pH value is 7.2~7.6) resuspended (concentration is about 3×10 10 cfu / mL) in a refrigerator at 4°C until use.

[0050] Pre-treat the collected mud samp...

Embodiment 2

[0063] (1) Preparation of microbial preparations for eliminating gram-positive pathogenic bacteria in aquatic products

[0064] The preparation process is the same as step (2) of Example 1, the only difference being that adding physiological saline to resuspend the Bdellovibrio sediment, the concentrations of the Bdellovibrio BDS01 bacterium liquid and the Bdellovibrio BDS02 bacterium liquid are respectively 10 10 pfu / mL, mix Bdellovibrio BDS01 bacterial solution and Bdellovibrio BDS02 bacterial solution according to the number of bacteria 1:1 to obtain the microbial preparation.

[0065] (2) Microbial preparations are used to eliminate Gram-positive pathogenic bacteria in aquatic products before consumption

[0066] ① Preparation of Gram-positive pathogenic bacteria contained in aquatic products

[0067] Mycobacterium tuberculosis (Mycobacteri μm tuberculosis ATCC607, purchased from China Common Microorganism Culture Collection Center), using liquid culture medium (take tend...

Embodiment 3

[0073] The microbial preparation containing only Bdellovibrio BDS02 is used to eliminate Gram-positive pathogenic bacteria during the transportation of aquatic products. The specific process is as follows:

[0074] The specifications of the test container, test water and test used tilapia, vannamei, river clam and hairy crab are all the same as in Example 1. Put 4 intact grids in each aquarium, put tilapia, vannamei, river clams and hairy crabs in each grid, and immediately put it flat in the aquarium after sealing the lid of the grid , Drain the excess water in the tank so that the water level just covers the grid. Before the experiment, it was sterilized by sodium dichloroisocyanurate, and the water temperature was 26 °C. During the experiment, it was continuously inflated with water. 3 aquariums were used as a control group, and only the aquatic product Gram-positive pathogenic bacteria liquid prepared in step (3) of Example 1 was added, and the final concentration was 1 ...

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Abstract

The invention discloses a bdellovibrio bacteriovorus bacterial strain eliminating aquatic product Gram-positive pathogenic bacterium and an application thereof. The invention separates to obtain bdellovibrio bacteriovorus BDS02 the length of flagellum of which is at least 1.8mu m. The bdellovibrio bacteriovorus with flagellum on the end is cambered single cell the size of which is 0.65*0.2 mu m when observed by an electron microscope. The two layer plating method is used for cultivating the bdellovibrio bacteriovorus bacterial strain at the temperature of 28 DEG C for four days to form transparent round negativecolony the diameter of which is 1-2mm. Before the microorganism preparation prepared by the bdellovibrio bacteriovorus BDS02 is eaten by aquatic products, common Gram-positive pathogenic bacteria comprising Mycobacterium tuberculosis, star-shaped nocardia asteroids, rhodococcus erythropolis, streptococcus faecalis, staphylococcus epidermidis, clostridium perfringens and corynebacterium glutamicum in the transportation process and cultivation process can be eliminated, which improves the safe factor of raw aquatic products for consumers, protects the health of the consumers and provides guarantee for greenly processing and producing the aquatic products.

Description

technical field [0001] The invention relates to a Bdellovibrio, in particular to a Bdellovibrio strain for eliminating Gram-positive pathogenic bacteria in aquatic products and application thereof. Background technique [0002] In recent years, major food safety incidents have occurred successively around the world, which not only brought huge economic losses, but also tested social stability and safety, making food safety issues become an issue of increasing concern. In this regard, countries and organizations around the world have set up a variety of food safety-related laws and regulations to achieve the purpose of preventing and controlling food safety problems. Microorganisms are one of the important factors that contaminate food. Due to their small size, wide distribution, strong viability, and rapid reproduction, how to avoid and reduce microbial contamination has become the primary issue in food processing and distribution. [0003] Breeding diseases have always bee...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23L1/015A01N63/02A01P1/00C02F3/34C12R1/01A23L5/20
Inventor 蔡俊鹏张敏
Owner SOUTH CHINA UNIV OF TECH
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