Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Duck gizzard duck's gizzard antioxidative peptide and application thereof

A technology of antioxidant peptide and duck gizzard protein, which is applied in the field of peptides to achieve the effect of wide source of raw materials, scientific and reasonable process, and high activity

Inactive Publication Date: 2016-08-03
GUIZHOU UNIV
View PDF6 Cites 13 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] The inventors have found in experiments that using duck gizzard as a raw material, the technology research of using enzymatic hydrolysis technology to prepare antioxidant peptides is in a blank stage, and using ORAC as a free radical antioxidant separation and purification index to prepare high-efficiency for duck gizzard enzymolysis products Active antioxidant peptides and their applications have not been reported

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Duck gizzard duck's gizzard antioxidative peptide and application thereof
  • Duck gizzard duck's gizzard antioxidative peptide and application thereof
  • Duck gizzard duck's gizzard antioxidative peptide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0057] 1) Thaw the Sansui duck gizzard, remove the tendons, chop it, and crush it with a grinder.

[0058] 2) Add petroleum ether to the minced duck gizzard meat for defatting for 10-12 hours, pour out the petroleum ether, completely volatilize the residual petroleum ether in a fume hood, collect the solids, and store them in bags in the refrigerator for later use.

[0059] 3) Homogenize the degreased minced duck gizzard with water at a ratio of 1:2~4, and adjust the pH value to 6.5~7.

[0060] 4) Adjust the temperature of the water bath to 50-55°C, add 0.4-0.6% papain to the defatted minced meat liquid, and then bathe the homogenate in constant temperature water for 3-5 hours.

[0061] 5) Inactivate the homogenate of duck gizzard after water bath at 95~110°C for 10~15min, after cooling, centrifuge at 4000~6000r / min for 10~20min, take the supernatant to obtain the enzymatic solution, freeze-dry to obtain the enzyme Lyophilized powder.

[0062] 6) The enzymatic hydrolyzate ob...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses duck gizzard duck's gizzard antioxidative peptide and application thereof. The amino acid sequence of the duck gizzard duck's gizzard antioxidative peptide is as shown in Asn-Ly-Phe-Ile-Leu-Lys (a component DKFILK), and the monoisotopic peak of a parent ion of the sequence is m / z 382.2376 Da. The preparation process comprises the following steps: degreasing duck gizzard, performing enzymolysis, performing ultrafiltration on an enzymolysis product, performing gel filtration chromatography, performing reverse high performance liquid chromatography purification, and performing mass spectrum together with amino acid analysis, thereby obtaining the antioxidative peptide. The duck gizzard duck's gizzard antioxidative peptide is wide and cheap in raw material source and scientific and reasonable in process; by virtue of an enzymolysis technique and together with purification through ultrafiltration grading and chromatographic analysis, the prepared antioxidative peptide has relatively high activity; compared with an antioxidant prepared through chemical synthesis, the antioxidative peptide disclosed by the invention has the advantages of security, no toxic or side effect, good antioxidation property, easiness in digestion and absorption, and the like, and can be used as medicines, functional food and the like.

Description

technical field [0001] The invention belongs to the technical field of polypeptides, and in particular relates to an antioxidant peptide derived from duck gizzard protein and a preparation method thereof, and also relates to an application of the antioxidant peptide derived from duck gizzard protein. Background technique [0002] More and more studies have shown that active oxygen free radicals in vivo have certain functions, such as immune and signal transduction processes. But too much active oxygen free radicals can also have a destructive effect, leading to damage to normal cells and tissues of the human body, thereby causing various diseases, such as heart disease, Alzheimer's disease, Parkinson's disease and tumors. Therefore, research on natural antioxidants that can remove excess active oxygen free radicals in the body, protect the normal structure and function of cells and mitochondria, prevent lipid peroxidation, and have other biological activities such as anti-ca...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C07K14/465C12P21/06C07K1/36C07K1/34C07K1/20A23J1/02A23J3/34A61K38/08A61P39/06
CPCC07K14/465A23J1/02A23J3/341A61K38/00C12P21/06
Inventor 苏伟文飞母应春杨旭会齐琦邱树毅
Owner GUIZHOU UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com