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A kind of inactivated vaccine of mycoplasma synovial bursa

A technology of mycoplasma synovialis and inactivated vaccine, which is applied in the directions of vaccines, veterinary vaccines, and antibody medical components to achieve the effects of preventing epidemics, making up for market vacancies, and reducing morbidity.

Active Publication Date: 2020-01-03
YEBIO BIOENG OF QINGDAO
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are commercial vaccines abroad; there is no Mycoplasma synovialum vaccine in the market in China

Method used

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  • A kind of inactivated vaccine of mycoplasma synovial bursa
  • A kind of inactivated vaccine of mycoplasma synovial bursa
  • A kind of inactivated vaccine of mycoplasma synovial bursa

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1, the screening of bacterial strain

[0022] 1 Culture medium for the production of Mycoplasma synovialis

[0023] 1.1 Preparation of improved CM liquid medium Take 21.0g of PPLO broth, 5g of glucose, add to 1000ml of deionized water, and 1ml of 1% phenol red solution. Add 200ml of inactivated horse serum, 200ml of 25% yeast extract, 1.2ml of 800,000 units / ml penicillin solution, 30ml of 1% coenzyme I solution, 30ml of 1% L‐cysteine ​​solution, and use 1mol / L sodium hydroxide Adjust the pH value of the solution to 7.6-7.8, and store at 2-8°C.

[0024] 1.2 Preparation of improved CM solid medium Take 21.0g of PPLO broth, 5g of glucose, 15.0g of agar powder and add deionized water to 1000ml, mix the above ingredients and heat to dissolve, quantitatively dispense, sterilize at 116°C for 30 minutes, put in 2 Store at ~8°C. Heat and dissolve 100ml of solid medium before use. When the temperature drops to about 60°C, add 20.0ml of inactivated horse serum, 20ml ...

Embodiment 2

[0052] Embodiment 2: production method of mycoplasma synovial bursa inactivated vaccine

[0053] 1 material

[0054] 1.1 Strain The Mycoplasma gallinarum YBF-MS1 strain was used to prepare the vaccine.

[0055] 1.2 Medium The improved CM liquid medium was provided by Qingdao Yibang Bioengineering Co., Ltd.

[0056] 1.3 Detection reagents Plate agglutination antigen, positive serum and negative serum were all provided by Qingdao Yibang Bioengineering Co., Ltd.

[0057] 1.4SPF chickens were purchased from Beijing Meria Weitong Experimental Animal Technology Co., Ltd.

[0058] 1.5 shearing machine T25 type, produced by German IKA company.

[0059] 1.6 High-speed refrigerated continuous flow centrifuge GR22GIII, purchased from Hitachi, Japan.

[0060] 2 methods

[0061] 2.1 Preparation of seeds for production

[0062] 2.1.1 Primary seed propagation Reconstitute the freeze-dried seeds of E3, E7, and E12 generations of Mycoplasma gallinarum YBF-MS1 strain with improved CM liqu...

Embodiment 3

[0129] Embodiment 3, the mycoplasma synovialis inactivated vaccine (YBF-MS1 strain) prepared by the present invention single-dose inoculation, single-dose repeated inoculation and once over-dose inoculation safety test:

[0130] 1 material

[0131] 1.1 The 21-day-old and 200-day-old SPF chickens used in the experiment were all purchased from Beijing Merial Weitong Experimental Animal Technology Co., Ltd., and were repurchased one week in advance and then isolated in the experimental animal room of Qingdao Yibang Bioengineering Co., Ltd. under negative pressure Feed in the container and use after observing no abnormalities.

[0132] 1.2 Vaccine Mycoplasma synovialis inactivated vaccine (YBF-MS1 strain), trial-produced by the laboratory, batch numbers are: 1201, 1202, 1203.

[0133] 2 methods

[0134] 2.1 Single-dose vaccination safety test Take 21-day-old SPF chickens and divide them into 2 groups, 10 in each group. Group 1 was subcutaneously injected with 1201 batches of in...

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Abstract

The invention discloses inactivated vaccine for chicken bursa synovialis mycoplasma. The inactivated vaccine comprises antigen and vaccine adjuvant, wherein the antigen is an inactivated YBF-MS1 strain and preserved in China Center for Type Culture Collection, Wuhan University, Wuhan City, the preservation date is 3<rd>, March, 2016, and the preservation number is CCTCC M 2016080. By means of the vaccine, a high-level antibody can be generated, the persistent period is long, cross protection performance is achieved, after immunization is completed, the morbidity of chicken bursa synovialis mycoplasma is obviously lowered, and epidemicity of chicken bursa synovialis mycoplasma can be effectively prevented. Domestically, no chicken bursa synovialis mycoplasma vaccine is on the market, it is the key to prevent and control the disease to prepare the inactivated vaccine for chicken bursa synovialis mycoplasma from the chicken bursa synovialis mycoplasma YBF-MS1 strain, and meanwhile market vacancy can be made up.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and more specifically relates to an inactivated vaccine of Mycoplasma gallinarum. Background technique [0002] Mycoplasma synoviae (Mycoplasma synoviae, MS), also known as chicken infectious synovitis, is an acute or chronic infectious disease caused by Mycoplasma synoviae in chickens and turkeys. Chicken infectious synovitis was first reported by Olson et al. (1964). The disease mainly affects joint synovial fluid and tendon sheath, which is characterized by swelling of joints, tendon sheaths and soles. Chicken flocks infected with the disease can cause obvious lameness, growth retardation and carcass degradation, etc. The disease can be transmitted vertically, causing the disease in chicks hatched from eggs produced during the disease period of the breeder chickens. Mycoplasma synovialis infection has spread all over the world and is distributed worldwide. It mainly af...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K39/02A61K39/39A61P31/04
CPCA61K39/0241A61K2039/521A61K2039/552
Inventor 马爽范根成孙健于国营宋新宇郭莉莉窦小龙程增青赵鹏郭伟伟
Owner YEBIO BIOENG OF QINGDAO
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