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Preparation method and application of label-free type electrochemical immunosensor based on MoS2/Au composite material

An immune sensor and composite material technology, applied in scientific instruments, analytical materials, material analysis through electromagnetic means, etc., can solve the problems of unsuitable rapid detection, large sample consumption, complex pretreatment, etc., and achieve low detection limit , simplified preparation process, good catalytic effect

Inactive Publication Date: 2016-07-27
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have high sensitivity and selectivity, but the detection process requires expensive special instruments, complex pretreatment and cumbersome operations, and large sample consumption, which is not suitable for rapid detection

Method used

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  • Preparation method and application of label-free type electrochemical immunosensor based on MoS2/Au composite material
  • Preparation method and application of label-free type electrochemical immunosensor based on MoS2/Au composite material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. A MoS-based 2 Preparation method and application of label-free electrochemical immunosensor based on / Au composite material:

[0031] (1) Use 1.0μm, 0.3μm, 0.05μm Al 2 O 3 Polishing powder successively grinds a glassy carbon electrode (GCE) with a diameter of 4mm, ultrasonically cleans it in ethanol and ultrapure water for 3 minutes, and blows it dry with nitrogen, and takes 6~10μL MoS 2 / Au nanocomposite material dispersion is dripped onto the electrode surface, dried at room temperature to form a film, washed with PBS (pH 7.4) buffer solution to remove unbonded MoS 2 / Au gets Au / MoS 2 / GCE;

[0032] (2) Take 10μL of 8~10μg / mL alpha-fetoprotein antibody (anti-AFP) standard solution and drip it onto the electrode surface, incubate overnight at 4℃, and rinse with PBS (pH7.4) buffer solution to obtain anti-AFP / Au / MoS 2 / GCE;

[0033] (3) Take 10 μL of bovine serum albumin (BSA) solution with a mass fraction of 1 to 3% and apply it to the surface of the electrode, inc...

Embodiment 2

[0035] Example 2 . MoS 2 / Au dispersion preparation:

[0036] Under stirring, add 10μL 0.01MHAuCl 4 The solution is added to 2mLMoS 2 (1mg / mL) solution, ultrasonic for 10min, stirring for 2h (repeat three times), quickly add 25μL of 0.01M NaBH freshly prepared 4 Solution, stir for 20min, add 10μL 0.01M sodium citrate solution dropwise, stir for 30min, centrifuge and wash with deionized water to obtain MoS 2 / Au dispersion.

Embodiment 3

[0037] Example 3 . AFP detection:

[0038] (1)AFP / BSA / anti-AFP / Au / MoS 2 / GCE is the working electrode, the Ag / AgCl electrode is the reference electrode, the platinum electrode is the counter electrode, H 2 O 2 For electrochemical probes, use electrochemical workstation for testing;

[0039] (2) Use it curve method to detect a series of different concentrations of AFP standard solutions and electrodes that do not specifically bind to AFP, the potential of the scan is -0.64V, record the it curve, according to the obtained current value and the logarithm of the AFP concentration Show a linear relationship, draw a working curve;

[0040] (3) Replace the AFP standard solution with the sample solution to be tested for testing.

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Abstract

The invention relates to the technical field of electrochemical immune sensing and particularly relates to a preparation method and application of a label-free type electrochemical immunosensor based on a MoS2 / Au composite material. H2O2 is used as an electrochemical probe and the MoS2 / Au composite material is used as a substrate material; and the stability and sensitivity of the immunosensor are remarkably improved based on the advantages of good film-forming capability and biocompatibility, large specific surface area, and excellent catalytic properties on the H2O2 by MoS2 and the like. A detection principle utilizes the change of current before and after an antigen-antibody immune reaction. After an antigen is reacted with an antibody modified on the current, an immune compound is formed and is a non-active substance, so that a channel from an electron to the surface of an electrode is seriously hindered. Along the increasing of the concentration of the antigen, more immune compounds are formed, and the channels from the electron to the surface of the electrode are fewer, so that a current signal is reduced. The sensor is used for detecting alpha fetoprotein; and a detection linear range is 0.005ng / mL-100ng / mL and the detection limit is 2.5pg / mL.

Description

technical field [0001] The invention relates to the technical field of electrochemical immunosensing, in particular to a MoS-based 2 Preparation method and application of label-free electrochemical immunosensor of / Au composite material. with H 2 o 2 As an electrochemical probe, MoS 2 / Au composite material as the base material, based on its good film-forming ability and biocompatibility, large specific surface area, and MoS 2 to H 2 o 2 The excellent catalytic performance and other advantages significantly improved the stability and sensitivity of the immunosensor. The sensor is used for the detection of alpha-fetoprotein, the detection linear range is 0.005ng / mL~100ng / mL, and the detection limit is 2.5pg / mL. Background technique [0002] Alpha-fetoprotein (AFP) is an important tumor marker. When liver cells become cancerous, the content of AFP in serum will increase sharply, and AFP in serum will increase, and it can also appear in teratoma, testis and ovary Tumors...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/327
CPCG01N27/327
Inventor 花小霞周长利夏方诠
Owner UNIV OF JINAN
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