Formaldehyde-removing and deodorizing ferment smell-cleaning agent and preparation method and application thereof
A deodorant and deodorant technology, applied in the direction of deodorization, separation methods, chemical instruments and methods, etc., can solve the problems of secondary pollution, complex extraction process of plant deodorants, high cost of chemical deodorants, etc.
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Embodiment 1
[0032] An enzyme formaldehyde-removing, deodorizing and deodorizing agent is prepared by the following method:
[0033] (1) Inoculate Issakuria orientalis into liquid medium A, culture and stand for 72 hours at 37°C to obtain Issakia orientalis culture medium, and the effective number of viable bacteria in the culture medium is 3.6×10 8 a / mL;
[0034] (2) Inoculate lactic acid bacteria paracasei into liquid culture medium A, shake and culture at 37°C for 36 hours, the lactic acid bacteria paracasei culture fluid, the effective number of viable bacteria in the culture fluid is 2.3×10 8 a / mL;
[0035] (3) Inoculate Pediococcus pentosaceae in liquid medium B, shake and culture at 37°C for 48 hours to obtain Pediococcus pentosaceae culture solution, the effective number of viable bacteria in the culture solution is 7.5×10 8 a / mL;
[0036] (4) Inoculate baker's yeast in liquid culture medium A, shake and cultivate at 37°C for 48 hours to obtain baker's yeast culture solution, an...
Embodiment 2
[0045] An enzyme formaldehyde-removing, deodorizing and deodorizing agent is prepared by the following method:
[0046] (1) Inoculate Issaccharomyces orientalis, Lactobacillus paracasei, Pediococcus pentosaceus and baker's yeast into the liquid medium according to the ratio of the number of bacteria 1:1:1:1, and culture it statically at 37°C to make the culture medium The total number of viable bacteria in the 9 cells / mL, the culture solution was centrifuged at 4°C and 3500rpm, and the supernatant was collected;
[0047] (2) Add the supernatant of step (1) to a dialysis membrane with a molecular weight cut-off of 14000, and put it into a phosphate buffer (9.46 g of disodium hydrogen phosphate in water, adjusted to pH 7.0 with potassium dihydrogen phosphate solution , constant volume to 1L), dialyze to remove low-molecular-weight substances at 5° C. under magnetic stirrer stirring conditions, and recover the substances in the dialysis membrane, which is the formaldehyde-removi...
Embodiment 3
[0053] For convenience of description, the deodorant prepared by the method of the above-mentioned Example 1 is named deodorant A, and the deodorant prepared by the method of Example 2 is named deodorant B.
[0054] Gas detection tube detection deodorization effect:
[0055] At room temperature, add 70ppm trimethylamine into a 1L brown glass bottle, and then spray 1ml of deodorant A and B respectively. After 10 minutes, 1 hour, 3 hours, 5 hours, and 24 hours, extract 50ml of the gas in the container, and add an amine detection tube to detect the concentration of the amine gas. The same operation was carried out with the deodorizing agent A and B with the culture medium not inoculated with microorganisms as a control group. Compared with the control group, the deodorants A and B of the present invention can completely digest and decompose trimethylamine within 1 hour, and have excellent deodorizing properties.
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