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Hair regeneration method for mesenchymal stem cell-induced alopecia areata and application

A technology of mesenchymal stem cells and alopecia areata, applied to medical preparations containing active ingredients, pharmaceutical formulas, preparations for in vivo experiments, etc.

Inactive Publication Date: 2016-07-20
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, there is no literature report on the study and application of subcutaneous injection of MSC to promote hair follicle reconstruction and hair regeneration in mice with alopecia areata

Method used

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  • Hair regeneration method for mesenchymal stem cell-induced alopecia areata and application
  • Hair regeneration method for mesenchymal stem cell-induced alopecia areata and application
  • Hair regeneration method for mesenchymal stem cell-induced alopecia areata and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Example 1: Identification of MSC supernatant secretions

[0048] MSC cells expanded to a total of 10 7 , when the fusion rate is 70-80%, start to change to serum-free medium and continue to culture for 12 hours, collect serum-free medium for protein chip cytokine detection, and use RayBio human cytokine antibody chip of Kangcheng Company to detect nearly 500 kinds of cytokines in total , and the specific operation steps are carried out according to the manual provided by Kangcheng Company.

[0049] After the X-ray film is exposed, scan the image on the film with a scanner and convert it into a grayscale TIFF image file for storage, such as Figure 4 As shown, the arrow in the lower row refers to VEGF, and the arrow in the upper row refers to FGF-7.

[0050] Run the ScanAlyze software to convert the dot matrix of the grayscale TIFF format image into digital data, store the original data as a Microsoft Excel file, and correct the original value minus the mean value of a...

Embodiment 2

[0064] Embodiment 2: mouse alopecia areata model preparation

[0065] 1. Alopecia areata model preparation

[0066] All animal experiments complied with the NIH Laboratory Animal Requirements. The experimental animals were all 6-week-old C3H / HeJ mice, male or female, weighing about 30 g, provided by the Experimental Animal Center of Second Military Medical University.

[0067] Firstly, imiquimod was used to induce the back skin of C3H / HeJ mice to form alopecia areata model. The method is as follows: take out the mouse carefully each time, take about 0.05 g of imiquimod cream with a clean medical cotton pad, and apply it evenly to the skin of the nape, with an area of ​​about 1.5×1.5 cm, once every two days. The longest continuous medication does not exceed 10 weeks. Clean the local area with normal saline before each administration to remove the residual drug from the previous administration. During the operation, the action should be as gentle as possible, and it can be a...

Embodiment 3

[0084] Example 3: Recovery and transplantation of human mesenchymal stem cells Recovery and transplantation of human mesenchymal stem cells

[0085] Human mesenchymal stem cells (MSC) can be isolated and purified according to the following literature methods: Reference: Minjuan Wu, Xiaocan Guo, Ling Yang, Yue Wang, Ying Tang, Yongji Yang, Houqi Liu. Mesenchymal Stem Cells with Modification of JAM-A Induce Hair Formation. StemCells Translational Medicine. 2014Apr; 3 (4): 481-8). The MSCs used in this example were separated and purified according to the aforementioned method, and then cryopreserved by the Department of Histology and Embryology of the Second Military Medical University where the inventor works.

[0086] The MSCs were taken out from the liquid nitrogen storage tank and placed in a 37°C water bath until they were dissolved. Centrifuge at 1000rpm for 5min, discard the supernatant, add 1ml of DMEM (L) culture solution containing 20% ​​FBS (L is low-sugar medium) to s...

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Abstract

The invention relates to the technical field of stem cells and hair loss treatment, and in particular relates to a hair regeneration method for mesenchymal stem cell-induced alopecia areata and an application. The invention provides a new application of the mesenchymal stem cells, namely the application of the mesenchymal stem cells in preparation of products for hair regeneration of induced alopecia areata, the hair regeneration method for the mesenchymal stem cell-induced alopecia areata, and an animal model for hair regeneration after alopecia areata built by the method. The hair regeneration method can be applied to hair follicle regeneration research and alopecia areata treatment.

Description

technical field [0001] The invention relates to the technical field of stem cells and hair loss treatment, in particular to a method for inducing hair regeneration in alopecia areata by mesenchymal stem cells, and the method is applied to the research on hair follicle regeneration and the treatment of alopecia areata. Background technique [0002] Hair has important physiological and social functions, but lifestyles such as huge social competition pressure, continuous mental tension, and staying up late for a long time will seriously damage the health of hair follicles and cause hair loss. Epidemiological surveys found that patients with hair loss are younger, and young and middle-aged people have become the "main force" in the hair loss population. Although the current understanding and understanding of hair loss is increasing and deepening, and supportive or adjuvant treatment measures have also progressed, more effective and comprehensive treatment methods are still expec...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61P17/14A61K31/4745A61K49/00
CPCA61K35/28A61K31/4745A61K49/0008
Inventor 刘厚奇仵敏娟王斐王越徐辰徐莎熊俊李侨
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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