Marking vaccine and serological identification method for porcine reproductive and respiratory syndrome high in pathogenicity

Abstract

The invention relates to a marking vaccine for the porcine reproductive and respiratory syndrome high in pathogenicity and a preparation method thereof.A virus seed produced through the vaccine is a constructed JXA1-RM strain, safety and immunogenicity of the JXA1-RM strain are maintained, in addition, the strain is good in stability, and the content of viruses produced by cells is high; after a pig is immunized through the vaccine, no sequence antibody against the M protein linear list position peptide amino acid sequence of the PRRS virus of an American strain is generated, and the vaccine can be used for differentiating an immunized animal from a naturally-infected animal.By means of a method for identifying and diagnosing the strain, antibodies generated by a field strain and a vaccine strain can be diagnosed, a conventional ELISA detection method is combined, an animal immunized through the vaccine and a naturally-infected animal can be differentiated, and it is beneficial to purification of the porcine reproductive and respiratory syndrome high in pathogenicity.

Description

technical field [0001] The invention relates to a highly pathogenic porcine reproductive and respiratory syndrome live vaccine and a preparation method thereof, which can realize the distinction between immunity and natural infection through a serological differential diagnosis method, and belongs to the field of veterinary biological products. Background technique [0002] Highly pathogenic porcine reproductive and respiratory syndrome (commonly known as highly pathogenic porcine reproductive and respiratory syndrome) is an acute and highly fatal contact infection of pigs caused by a variant strain of porcine reproductive and respiratory syndrome virus (PRRSV) sick. According to the 2008 version of the "Terrestrial Animal Diagnostic Test and Vaccine Manual" formulated by OIE, the disease is listed as one of the legally reported animal diseases, and is listed as "Class I Animal Disease" in my country, which is a threat to my country's pig industry. one of the major infectiou...

AI Technical Summary

Problems solved by technology

These strains can resist the attack of classic PRRS and highly pathogenic PRRS virus strains, and have played an important role in the prevention and control of highly pathogenic PRRS in my country. However, in recent years, highly pathogenic PRRS PRRS has become a sporadic trend, which still causes huge economic losses. The original vaccine cannot distinguish immunity from natural infection, and is still using vaccines to prevent highly pathogenic PRRS in countries or regions. They want to purify them Reach the purpose of controlling and eliminating highly pathogenic PRRS, but there is no corresponding method that can distinguish immunity and natural infection, so that the effect of prevention is always unsatisfactory. Now it is urgent to develop a kind of ability to distinguish immunity and natural infection (DIVA, DiscriminationofInfectedfromVaccinatedAnimals) vaccine

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