A method for identifying the age of cephalopods using horny jaws

Abstract

The invention provides a method for identifying the age of cephalopods by using horny jaws, including grinding the longitudinal section of the horny jaws and counting growth lines, the main steps are extraction, cleaning, soaking, cutting, placing, resin embedding, Cutting, fixation on glass slides, grinding, polishing, picture making, and counting of growth lines; grinding the longitudinal section of the horny jaw, that is, grinding from the left and right sides to the midline to the core surface; CCD takes pictures to ensure that the growth lines can be read at any time in the future; the growth lines are counted from the rear end of the beak to the front along the direction perpendicular to the growth lines to ensure the accuracy of counting; the horny jaw is not easy to grind, and the grinding is fragile And the problem that growth lines are not easy to observe and count.

Description

technical field [0001] The invention belongs to the field of fisheries, and mainly relates to a method for identifying the age of cephalopods by using horny jaws. Background technique [0002] Cephalopods belong to molluscs, are generally annuals, and have the characteristics of dying after laying eggs. They are important marine economic species and are widely distributed in the world's oceans and Antarctic sea areas. [0003] Age determination is an important research topic in cephalopod fishery biology and ecology. The age identification methods of cephalopods can be divided into indirect and direct methods. Due to the characteristics of cephalopods such as fast growth, long spawning period, and complex population structure, the indirect estimation of age by carcass length or body weight is not accurate enough. Among all the direct methods, the use of growth lines in hard tissues is considered to be the most effective method for age identification of cephalopods. [000...

AI Technical Summary

Problems solved by technology

In the prior art, scissors are usually used to directly cut the beak of the horny jaw with scissors in the production of slices of the horny jaw of cephalopods. This operation is likely to cause the fracture of the beak section and affect the interpretation of growth lines; secondly, the grinding of the beak of the horny jaw is direct grinding , on the one hand, it will cause the cuticle to break during the grinding process, and on the other hand, it will cause the offset of the grinding plane, which will increase the damage to the cutin during the grinding process, and the success rate of slicing is low.

In addition, in the prior art, counting the growth lines of the horny palate is directly observed under a microscope, and it is extremely inconvenient to count the growth lines; The growth lines at the junction of the dorsal side and the re-measurement (the dotted line in the figure), and this counting method will mistake some sub-daily lines for sun lines, resulting in a large error

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