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Method for extracting high-purity resveratrol from wild blueberry of Great Khingan by enzymolysis

A technology of resveratrol and wild blueberry, applied in chemical instruments and methods, preparation of organic compounds, organic chemistry, etc., can solve the problems of unfavorable resveratrol chemical structure stability, long time for enzymatic hydrolysis reaction at constant temperature, heat preservation and low temperature. High sealing requirements, etc., to achieve the effect of improving the yield of resveratrol, shortening the production cycle, and moderate boiling point

Inactive Publication Date: 2016-06-22
DAXINGANLING PERFECTION NOBILITY FRIGID ZONE BIO TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The main disadvantages of this method are: 1. the constant temperature enzymatic hydrolysis reaction time is long, and the high temperature for a long time is not conducive to the chemical structure stability of resveratrol; Therefore, drying operation after enzymolysis is also unfavorable for the chemical structure stability of resveratrol; 3. because acetone has a low boiling point, is volatile, has certain toxicity, and is prone to explosion hazard when mixed with air, therefore, when it is used as a countercurrent extraction solvent, The insulation and sealing requirements of the corresponding equipment are relatively high; ④Ethyl acetate-petroleum ether is used as the separation and elution solvent, of which petroleum ether is highly toxic and flammable, and the solvent recovery process involves a large safety hazard

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1: The present invention provides a method for enzymatically extracting high-purity resveratrol from Daxinganling wild blueberries. The specific steps of the method are as follows:

[0025] (1) Biological enzymatic hydrolysis reaction

[0026] First put the blueberry fruit raw materials picked in Daxing’an Mountains into a sub-crusher for crushing, and then sieve through a 220-mesh sieve, collect the sieved blueberry juice, and set aside, and return the unsifted particles to the grinder for crushing. According to the ratio of the mass (g) of blueberry powder: the mass (mg) of cellulase: the volume (mL) of sodium acetate trihydrate buffer solution is the ratio of 1:2:20, first use high-concentration hydrochloric acid or sodium hydroxide solution After adjusting the pH value of the buffer solution to 4.7, the blueberry powder and the buffer solution after adjusting the pH value were placed in a batch reactor in sequence, stirred at a constant temperature of 45°...

Embodiment 2

[0034] Embodiment 2: The present invention provides a method for enzymatically extracting high-purity resveratrol from Daxinganling wild blueberries. The specific steps of the method are as follows:

[0035] (1) Biological enzymatic hydrolysis reaction

[0036] First, put the blueberry fruit raw materials picked in the Daxingan Mountains into a pulverizer for pulverization, then sieve through a 180-mesh sieve, collect the sieved blueberry juice, and set aside, and return the unscreened particles to the pulverizer for pulverization. According to the ratio of the mass (g) of blueberry powder: the mass (mg) of cellulase: the volume (mL) of sodium acetate trihydrate buffer solution is the ratio of 1:1:10, first use high concentration hydrochloric acid or sodium hydroxide solution After adjusting the pH value of the buffer solution to 5.0, the blueberry powder and the buffer solution after adjusting the pH value were placed in a batch reactor in sequence, stirred at a constant temp...

Embodiment 3

[0044] Embodiment 3: The present invention provides a method for enzymatically extracting high-purity resveratrol from Daxinganling wild blueberries. The specific steps of the method are as follows:

[0045] (1) Biological enzymatic hydrolysis reaction

[0046] First, put the blueberry fruit raw materials picked in the Daxingan Mountains into a pulverizer for pulverization, then sieve through a 200-mesh sieve, collect the sieved blueberry juice, and set aside, and return the unscreened particles to the pulverizer for pulverization. According to the ratio of the mass (g) of blueberry powder: the mass (mg) of cellulase: the volume (mL) of sodium acetate trihydrate buffer solution is the ratio of 1: 10: 50, first use high-concentration hydrochloric acid or sodium hydroxide solution After adjusting the pH value of the buffer solution to 6.0, the blueberry powder and the buffer solution after adjusting the pH value were placed in a batch reactor in sequence, stirred at a constant t...

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Abstract

The invention belongs to the field of natural organic chemistry, and relates to a method for enzymatically extracting high-purity resveratrol from Daxinganling wild blueberries. The invention uses the blueberry fruit picked in Daxing'an Mountains as a raw material, and obtains a high-purity resveratrol crystal powder product through a simplified process of biological enzymolysis reaction, water extraction, alcohol precipitation, deep extraction, separation, concentration and drying. The method of the invention has the characteristics of sufficient biological enzymolysis, short time consumption, low energy consumption cost, high operation safety, full use of raw materials and organic solvents, waste liquid mainly being water, good for environmental protection, and easy industrialization and application. The resveratrol crystal powder product prepared by the method of the invention has a high purity, which can reach more than 98 percent; and a high yield, which can reach 1.40 percent.

Description

technical field [0001] The invention belongs to the technical field of extracting resveratrol from natural plants, in particular to a method for preparing high-purity resveratrol from natural plant blueberries. Background technique [0002] Resveratrol is a non-flavonoid polyphenolic compound containing a stilbene structure, its chemical name is 3,4',5-trihydroxystilbene, and it is a white or light yellow crystalline powder. Resveratrol can not only prevent tumors and cancer diseases, but also prevent cardiovascular and cerebrovascular diseases. It has health effects such as liver protection, anti-oxidation, regulation of blood lipid balance, and anti-aging. It is widely used in medicine and health care. Since resveratrol is a polyphenolic compound of stilbene substances, which has phenolic and olefinic properties, under the condition of exposure to air or light, resveratrol will be oxidized or undergo isomerization transformation to form irreversible substances with physio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/22C07C39/21C07C37/82
CPCC12P7/22C07C37/82
Inventor 刘巍
Owner DAXINGANLING PERFECTION NOBILITY FRIGID ZONE BIO TECH CO LTD
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