Method for producing ophiocordyceps sinensis powder through rapid and deep liquid state fermentation

Abstract

The invention discloses a method for producing ophiocordyceps sinensis powder through rapid and deep liquid state fermentation. The method comprises the steps that fermentation liquor rich in mycelia is rapidly obtained from ophiocordyceps sinensis strains through a semi-continuous fermentation technology, and then the fermentation liquor is subjected to micro-filtration-membrane treatment and separation, so that ophiocordyceps sinensis mycelia are obtained. By means of the method, the traditional intermittent batch fermentation process for ophiocordyceps sinensis is changed, and in addition, the centrifugal separation mode is replaced with efficient ceramic membrane microfiltration for separation of the mycelia. By means of the semi-continuous fermentation process, the defects that the traditional batch fermentation process is large in number of process links, long in intermediate gap time, low in equipment efficiency and the like are overcome; the fermentation liquor is filtered through an efficient ceramic membrane, the situation that filtrate enters the mycelia can be reduced, the yield of the mycelia can be raised, COD of the filtrate is lowered, sewage treatment amount is decreased, cells are not destroyed, and it is guaranteed that all effective constituents in the cells are recycled. Compared with the traditional process, the method has remarkable economic benefits, social benefits and environment protection benefits.

Description

technical field [0001] The invention belongs to the technical field of microbes, and in particular relates to a method for rapidly producing fungal mycelia by deep liquid fermentation technology, more precisely, a method for rapidly producing Mortierella chinensis powder by deep liquid fermentation. Background technique [0002] Microbial fermentation can be divided into batch, fed-batch, semi-continuous, continuous and other modes. Batch fermentation consumes a lot of manpower and material resources. Each batch of fermentation needs to be loaded, sterilized, inoculated, discharged, and cleaned. The process is cumbersome, the fermentation cycle is long, and the production efficiency is low; Fed-batch fermentation Although the lack of nutrients or precursors can be supplemented by feeding, the product synthesis will inevitably be blocked due to the continuous accumulation of harmful metabolites; the production intensity of continuous fermentation is greatly improved compared ...

AI Technical Summary

Problems solved by technology

[0005] The traditional Chinese hairy sporophyte liquid fermentation process is mostly an intermittent batch fermentation process, but the batch fermentation process consumes a lot of manpower and material resources, and each batch of fermentation needs to be loaded, sterilized, inoculated, discharged, cleaned, etc. The operation is complicated, the process is cumbersome, the non-fermentation period is long, and the production efficiency is low; while the subsequent separation process is traditionally separated by a centrifuge. This method is subject to the influence of the separation factor of the centrifuge, and the mycelium is subjected to shear force during the separation process. Destruction, part of the active ingredients are lost into the filtrate, resulting in a decrease in product quality and yield. The separated filtrate is turbid and contains more broken mycelia, resulting in a high COD of the discharged filtrate. If the filtrate meets the sewage discharge standard, it needs to pass through Complex sewage treatment, the workload of the sewage treatment system is too large, which brings a serious economic burden to the enterprise

Images