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Preparation method of rape pollen polysaccharide

A technology of rape pollen polysaccharide and rape pollen, which is applied in the field of preparation of rape pollen polysaccharide, can solve the problems of high solvent consumption, unstable activity, and many extraction times, and achieve the effects of reducing energy consumption, saving raw materials, and using less

Inactive Publication Date: 2016-06-15
XIANGYANG LIGAOLE BIOCHEM TECH CO LTD
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

It also shows the disadvantages of many times of extraction and long time. It needs to be extracted for a total of 3 hours.
[0007] Based on the above documents, it is found that the extraction method used is basically complete, but at the same time, there are many shortcomings such as high solvent consumption, many extraction times, low purity, unstable activity, and serious pollution. In order to save solvent and energy consumption, reduce environmental pollution, and save Raw material, it is necessary to improve the extraction and preparation method of rapeseed pollen polysaccharide

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The selection of embodiment 1 extraction process condition

[0028] (1) The influence of the time of ultrafine pulverization and decomposition on the extraction effect of rapeseed pollen polysaccharide

[0029] Experimental method: take 500g of rapeseed pollen and decompose it by ultrafine pulverization, put it in a plant extraction tank, add 60% ethanol as the extraction solvent, the weight-to-volume ratio of pollen to solvent is 1:15, and the extraction temperature is 65°C. The decomposition time is extracted once, the extract after decomposition and extraction is filtered or centrifuged, the supernatant is concentrated, extracted with dichloromethane to obtain a thick paste, and dried to obtain the rapeseed pollen extract. The extraction effect of rapeseed pollen polysaccharides under different extraction time was investigated. The results are shown in Table 1:

[0030] Table 1

[0031] Time extraction rate (%)

[0032] 10h24.2

[0033] 14h30.1

[0034] 18h45.6...

Embodiment 2

[0077] Take 500g of rapeseed pollen milled with ultra-fine and nano water, put it in a plant extraction tank, the ethanol concentration is 60%, the weight-to-volume ratio of medicinal materials and solvent is 1:15, the extraction temperature is 60°C, and the extraction time is 120min. After suction filtration or centrifugation, the supernatant liquid is concentrated, dichloromethane extraction is prepared to obtain a thick paste, ethanol is added for C18-preparative chromatographic separation to obtain a thick paste of rapeseed pollen extract, and the rapeseed pollen polysaccharide is obtained by drying. Results: The dry paste yield was 6.5%, the content was 25.0%, and the extraction rate was 99.7%.

Embodiment 3

[0079] As shown in Example 2, the difference is that the weight-to-volume ratio of pollen to solvent in Example 2 is 1:20, while the weight-to-volume ratio of pollen to solvent in Example 3 is 1:10. Results: The yield of dry paste was 5.9%, the content was 16.7%, and the extraction rate was 91.7%.

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Abstract

The invention discloses a method for extracting rapeseed pollen polysaccharides by ultrafine pulverization, simple operation, high wall breaking rate, solvent-free operation, and beneficial for extraction of subsequent active components. It decontaminates rapeseed pollen, washes it clean, filters water, then breaks the wall by superfine pulverization, adds alcohol with low boiling point and other organic solvents, and carries out reflux three times for extraction. The method of combining special vacuum, microwave and low-temperature drying equipment is used to dry the rapeseed pollen extract, keeping the relevant active ingredients unchanged, reducing the drying temperature and speeding up the drying speed. Using macroporous adsorption resin C18 reversed-phase silica gel column chromatography-special preparative chromatography for separation and purification, a product with a content of 90% of rapeseed pollen extract is obtained. Further use our company to prepare a specific solvent for recrystallization to purify the rapeseed pollen extract with a purity of over 95%. The optimal process of extracting, extracting, drying, purifying and separating, and recrystallizing the rapeseed pollen extract compound was determined by orthogonal experiment. The preparation method of the invention has the advantages of relatively stable process operation, relatively high extract yield, high purity, no pollution to the environment, and is suitable for the requirements of modern industrial automation production.

Description

technical field [0001] The invention relates to a technique for extracting rapeseed pollen polysaccharide, in particular to a preparation method of rapeseed pollen polysaccharide. Background technique [0002] Rapeseed Pollen Pollen is rich in functional substances (including protein, vitamins, trace elements, flavonoids), these substances act synergistically on the body, regulate various functions of the body, balance nutrition in the body, enhance metabolism, and prevent capillary permeability obstacles. Pollen can also promote the development of endocrine glands, improve and regulate the secretion function of endocrine glands, so it has a therapeutic effect on diseases caused by endocrine dysfunction. [0003] Rapeseed pollen benefits the kidneys, strengthens the body, strengthens the waist, and contains high flavonols, which has the effects of anti-atherosclerosis, treating varicose ulcers, lowering cholesterol and anti-radiation. Pollen's health effects, pharmacologic...

Claims

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Application Information

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IPC IPC(8): C08B37/00
CPCC08B37/0003
Inventor 张军徐晓云李云捷和平顾红涛
Owner XIANGYANG LIGAOLE BIOCHEM TECH CO LTD
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