Preparation method of functionalized gold nanostar/siRNA complex stabilized by RGD-modified dendrimers
A dendrimer, gold nanotechnology, applied in recombinant DNA technology, medical preparations containing active ingredients, and other methods of inserting foreign genetic materials, etc. The effect of monodispersity, simple operation and low cost of raw materials
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0055] (1) Weigh 10mg G3.NH 2 Dissolve in 20mL of ultrapure water, and add 3.25μL of methylthioglycolate (Methylthioglycolate, 95%, Mw=106.14, ρ=1.187) to it, react in a water bath at 60°C for 2 days, and use a dialysis bag with a molecular weight cut-off of 1000 in water Partially mercaptolated G3.NH was obtained after three days of dialysis 2 (G3.NH 2 -SH), and stored at -20°C after lyophilization. Take 3 mg of freeze-dried G3.NH 2 and G3.NH 2 -SH dissolved in deuterated water for NMR test, from figure 1 As can be seen in G3.NH 2 -SH(b) compared to G3.NH 2 (a), there is an additional enhanced absorption peak at 3.25, which is the characteristic peak of the amide bond generated by the reaction between the amino group on the dendrimer and methyl thioglycolate, and it can be known by peak area integration that each G3. NH 2 15.51 sulfhydryl groups are connected on it.
[0056] (2) Weigh 100mg trisodium citrate, dissolve it in 9.9mL ultrapure water, take 1.5mL and add ...
Embodiment 2
[0059] Take the aqueous solutions of Au seeds and Au nanostars prepared in Example 1 respectively in 2mL centrifuge tubes, add 700 μL of ultrapure water therein, ultrasonically uniform, and measure the ultraviolet absorption (see figure 2 , UV absorption spectra of gold seeds and AuNSs at 400 to 1000 nm). The ultraviolet results show that the absorption peak of the gold seed (a) is around 520nm, while the ultraviolet absorption peak of the gold nanostar (b) synthesized by the seed growth method is around 800nm, indicating that the gold nanostar (b) with a unique absorption peak in the near-infrared region has been successfully synthesized. Gold nanostars.
[0060] Take 5 μL of the aqueous solutions of Au seeds and Au nanostars prepared in Example 1, respectively, and then prepare 100 μL of nanoparticle suspension with ultrapure water. Drop 5 μL of the nanoparticle suspension on the surface of the copper grid and dry it in the air for TEM testing (see image 3 , morphology a...
Embodiment 3
[0063] Mix 3.70mg 6-M with 20mg NH 2 -PEG-COOH was mixed in 5mL DMSO solution, stirred and reacted for 10 hours to obtain COOH-PEG-6-M; then 6.91mg RGD fully dissolved in 2mL DMSO was added dropwise to COOH-PEG-6-M In DMSO solution, stirred and reacted for 1 day, dialyzed with a dialysis bag with a molecular weight cut-off of 1000 for 3 days, and then freeze-dried to obtain COOH-PEG-RGD. The COOH-PEG-RGD that gets 3mg synthesis is dissolved in deuterated DMSO and carries out nuclear magnetic resonance analysis ( 1 H NMR) (see Figure 5a). It can be seen from the figure that the spectral peaks at 7.3 and 7.4ppm prove that RGD is successfully connected to PEG, and it can be seen from the peak area integration that 0.35 RGD is connected to each PEG.
PUM
Property | Measurement | Unit |
---|---|---|
diameter | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com