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Method for improving dissolvability of rice protein isolates by supersonic wave-assisted alkali treatment

A protein isolate, high solubility technology, applied in vegetable protein processing and other directions, can solve the problems of low dispersibility, poor solubility of rice protein isolate particles, lack of system solutions, etc. way safe effect

Inactive Publication Date: 2016-06-15
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, the solubility of rice protein isolate particles is poor and the problem of low dispersion has been reported, but there is still a lack of systematic solutions

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] Put 50L of 3wt% rice protein isolate solution in an ultrasonic treatment tank equipped with a constant temperature water bath, adjust the pH to 10 with sodium hydroxide solution, stir for 10 minutes to a uniform state, and perform ultrasonic treatment on the solution. The ultrasonic treatment time is 55 minutes and the frequency The frequency is 20KHz, the power is 500W, the ultrasonic intermittent time is 1s, the ultrasonic on time is 1s, and the solution temperature is maintained at 8°C during the treatment process; at the same time, the clear water used in the solution used in the treatment process is replaced with deionized water to reduce unnecessary ion interference; After ultrasonication, adjust the pH to 7.5 with hydrochloric acid solution, then centrifuge at 4°C and 8500g for 15 minutes, take the supernatant to obtain a highly soluble rice protein isolate solution, and pass the obtained supernatant through a reverse osmosis machine to desalt. The obtained desalt...

Embodiment 2

[0020] Put 50L 8wt% rice protein isolate solution in an ultrasonic treatment tank equipped with a constant temperature water bath, adjust the pH of the dispersion to 12 with sodium hydroxide solution, stir for 10 minutes to a uniform state, and perform ultrasonic treatment on the solution for 100 minutes , the frequency is 30KHz, the power is 1500W, the ultrasonic intermittent time is 1s, the ultrasonic on time is 1s, and the solution temperature is kept at 60°C during the treatment process; at the same time, the clear water used in the solution used in the treatment process is replaced with deionized water to reduce unnecessary ions Interference; after the ultrasound is finished, adjust the pH to 7.5 with hydrochloric acid solution, then centrifuge at 4°C and 8500g for 15 minutes, take the supernatant to obtain a highly soluble rice protein isolate solution, and remove the obtained supernatant through a reverse osmosis machine. salt to obtain a desalted rice protein isolate so...

example 3

[0023] Put 50L of 6wt% rice protein isolate solution in an ultrasonic treatment tank equipped with a constant temperature water bath, adjust the pH of the dispersion to 12 with sodium hydroxide solution, stir for 10 minutes to a uniform state, and perform ultrasonic treatment on the solution. The ultrasonic treatment time is 100 minutes , the frequency is 40KHz, the power is 1000W, the ultrasonic intermittent time is 1s, the ultrasonic on time is 1s, and the solution temperature is maintained at 90°C during the treatment process; at the same time, the clear water used in the solution used in the alkali treatment process is replaced with deionized water to reduce unnecessary Ion interference; after the ultrasonication, adjust the pH to 7.5 with hydrochloric acid solution, then centrifuge at 4°C and 8500g for 15 minutes, take the supernatant to obtain a highly soluble rice protein isolate solution, and pass the obtained supernatant through a reverse osmosis machine Desalination, ...

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Abstract

The invention discloses a method for improving dissolvability of rice protein isolates by supersonic wave-assisted alkali treatment. The method comprises the following steps: 2-10wt% of a solution of rice protein isolates is placed in an ultrasonic treatment groove with constant temperature water-bath; an alkaline solution is used for adjusting the pH value of a dispersion liquid to 9-13, and ultrasonic treatment is carried out for the solution; after the ultrasonic treatment, the pH value is adjusted to 7.5; a supernatant is taken by centrifugation, and a solution of rice protein isolates is obtained; reverse osmosis desalination and spray drying are carried out, and rice protein powder with high dissolvability is obtained. At the same time, all water used in the treatment process is deionized water, in order to reduce unnecessary ion interference. The method has the advantages of simple process, safe treatment mode, and substantially improved dissolving property of rice protein isolates; compared with untreated protein, the obtained protein solubility is increased by 30-48 times, so that dissolvability of rice protein isolates is improved to a large extent; rice protein isolates are suitable for various foodstuff ingredients and biological product ingredients.

Description

technical field [0001] The invention belongs to the field of ultrasonic-assisted protein modification, and in particular relates to a method for improving the solubility of rice protein isolate by ultrasonic-assisted alkali treatment. Background technique [0002] Rice protein is a by-product of rice starch and rice syrup produced from broken rice. It is a very ideal plant protein, containing all the amino acids needed by the human body, in line with the ideal model recommended by WHO / FAO, and has hypoallergenicity. Recognized as high-quality food protein. [0003] Although it has been recognized as a high-quality vegetable protein raw material, the poor solubility of rice protein leads to poor emulsification, foaming, gelling and other functional properties, which limits its wide application in food and related fields. Aiming at the limiting factor that the rice protein content is more than 75% of the water-insoluble glutenin, scientific researchers have carried out a seri...

Claims

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Application Information

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IPC IPC(8): A23J3/14
CPCA23J3/14
Inventor 张龙涛潘征缪松郑宝东黄旭辉王培森李庆旺蔡坤淇
Owner FUJIAN AGRI & FORESTRY UNIV
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