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Method for increasing yield of pullulan

A pullulan polysaccharide and yield technology is applied in the field of improving the yield of pullulan polysaccharide, which can solve the problems of difficult control of yield, long fermentation time of pullulan polysaccharide, etc., and achieves accelerated substrate conversion, increased intracellular production capacity, enhanced The effect of electron transfer

Inactive Publication Date: 2016-06-01
天津北洋百川生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the fermentation time of pullulan polysaccharide is long, and the output is also difficult to control

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1), seed medium: sucrose 90g / L, yeast powder 4g / L, sodium chloride 3g / L, anhydrous ammonium sulfate 1g / L, MgSO4 7H 2 O0.5g / L, K 2 HPO 4 3g / L, FeSO 4 ·7H 2 O0.05g / L, pH6.5; fermentation medium components: sucrose 150g / L, peptone 7g / L, sodium chloride 2.5g / L, MgSO 4 ·7H 2 O0.4g / L, K 2 HPO 4 6g / L, FeSO 4 ·7H 2 O0.05g / L, initial pH6.0.

[0027] (2) Activate the Aureobasidium pullulans strain for 6 hours, put it into a 500mL baffle bottle with a liquid content of 100mL, cultivate it at 30°C and 180rpm for 29h, and measure the OD 600 is 0.318. Connect it into a 5L fermenter with a liquid volume of 3.5L, and the culture condition is OD 600 When 600 When ≥0.5, the temperature is controlled at 28°C, the ventilation rate is 1.0 (V / V), and the rotation speed is 300rpm.

[0028] (3), real-time monitoring of fermentation broth OD 600 , OD 600 When 0.219, add 0.03% compound I, add 16h, OD 600 When it reaches 0.609, add 0.05% compound agent II, the total fermentation t...

Embodiment 2

[0031] (1), seed medium: sucrose 90g / L, yeast powder 4g / L, sodium chloride 3g / L, anhydrous ammonium sulfate 1g / L, MgSO4 7H 2 O0.5g / L, K 2 HPO 4 3g / L, FeSO 4 ·7H 2 O0.05g / L, pH6.5; fermentation medium components: sucrose 150g / L, peptone 7g / L, sodium chloride 2.5g / L, MgSO 4 ·7H 2 O0.4g / L, K 2 HPO 4 6g / L, FeSO 4 ·7H 2 O0.05g / L, initial pH6.0.

[0032] (2), after activating the Aureobasidium pullulans strain for 7 hours, insert it into the seed medium, cultivate it at 28° C. at 180 rpm for 32 hours, and when the OD600 is measured to be 0.301, insert the seed with a 3% inoculum size and the filling volume is 18 L In a 30L fermenter, the culture condition is OD 600 When 600 When ≥0.5, the temperature is controlled at 28°C, the ventilation rate is 1.0 (V / V), and the rotation speed is 300rpm.

[0033] (3) Real-time monitoring of fermentation broth OD 600 , OD 600 When it is 0.223, add 0.02% compound I, after adding 16h, OD 600 When it reaches 0.605, add 0.05% compound ag...

Embodiment 3

[0036] (1), seed medium: sucrose 90g / L, yeast powder 4g / L, sodium chloride 3g / L, anhydrous ammonium sulfate 1g / L, MgSO4 7H 2 O0.5g / L, K 2 HPO 4 3g / L, FeSO 4 ·7H 2O0.05g / L, pH6.5; fermentation medium components: sucrose 150g / L, peptone 7g / L, sodium chloride 2.5g / L, MgSO 4 ·7H 2 O0.4g / L, K 2 HPO 4 6g / L, FeSO 4 ·7H 2 O0.05g / L, initial pH6.0.

[0037] (2), after activating the Aureobasidium pullulans strain for 8 hours, insert in the first-class seeds, 32 ℃, 180rpm cultivate 28h, insert the first-class seeds with 3% inoculum amount into the second-class seeds, 28 ℃, ventilation rate 1.0( V / V), the rotation speed is 300rpm and cultivated for 16h, and the OD is measured 600 It is 0.323, it is inserted in the 200L fermentor of 150L with the inoculum size of 3%, and culture condition is OD 600 When 600 When ≥0.5, the temperature is controlled at 28°C, the ventilation rate is 1.0 (V / V), and the rotation speed is 300rpm.

[0038] (3) Real-time monitoring of fermentation brot...

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PUM

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Abstract

The invention discloses a method for increasing the yield of pullulan. The method comprises the following steps: after activating aureobasidium pullulans, inoculating a seed medium; after OD600 is greater than 0.3, inoculating seeds into a fermentation medium; monitoring OD600 of a fermentation liquid in real time; when OD600 is greater than 0.2 and less than 0.3, adding a compounding agent I which accounts for 0.02-0.03% of the volume of the fermentation liquid, so as to greatly increase the bacterium growth velocity and remarkably shorten the logarithmic growth phase; when OD600 is greater than or equal to 0.6, adding a compounding agent II which accounts for 0.03-0.05% of the volume of the fermentation liquid. The substrate conversion speed can be accelerated, the polysaccharide synthesis performance can be improved, and the yield can be greatly increased.

Description

technical field [0001] The invention relates to the technical field of microbial fermentation, in particular to a method for increasing the yield of pullulan. Background technique [0002] As a hydrophilic colloid with good solubility and excellent adhesion, pullulan has a wide range of applications in the fields of food, medicine, and chemical industry. However, the fermentation time of pullulan polysaccharide is long, and the yield is difficult to control. Most amino acids, vitamins and other growth factors can promote the growth of bacteria. Amino acids and vitamins are added in different types and amounts, and the promotion effect on the growth of bacteria is also quite different. Contents of the invention [0003] The technical problem to be solved by the present invention is to provide a method for increasing the yield of pullulan. [0004] The technical scheme that the present invention takes for solving the technical problem existing in known technology is: [...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/10
Inventor 乔长晟王萌李振海马正旺孙芳艳
Owner 天津北洋百川生物技术有限公司
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