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A high-temperature-resistant strong emulsifying microbial strain and its application in wax removal and anti-wax

A technology for removing wax and preventing bacteria, applied in the field of petroleum microorganisms, can solve the problems of poor high temperature resistance and other problems

Active Publication Date: 2019-07-26
唐山新新石油科技开发有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It has been widely used in countries with advanced technology in the world, and the research and application of microbial oil recovery technology are also being carried out in China, but most of the strains are poor in high temperature resistance. For example, the Chinese invention patent CN201210137276.5 discloses a strain resistant to high temperature paraffin degradation Bacteria and its application in wax removal and anti-wax, the wax removal rate of Microbacterium NS02 in this invention is 59%, and it can grow under the condition of 30~70℃

Method used

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  • A high-temperature-resistant strong emulsifying microbial strain and its application in wax removal and anti-wax
  • A high-temperature-resistant strong emulsifying microbial strain and its application in wax removal and anti-wax
  • A high-temperature-resistant strong emulsifying microbial strain and its application in wax removal and anti-wax

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0091] Screening and identification of strains with preservation number CCTCC No: M2015695

[0092] 210 oil and water samples were collected in the oil field, and 18 strains of Qingfangwax were obtained through enrichment culture, and then through directional domestication and compatibility optimization of crude oil, eight strains were finally selected, namely 98-32, 98-11, XG- 2. NG-23, SG-3, G104-5-1, G104-5-2 and G104-5-3.

[0093] Streak the selected strains on the nutrient agar plate, culture at 37°C for 48 hours, place at room temperature for 24 hours to observe and describe the characteristics of the colony and take pictures, select a typical single colony to make hanging drops, and observe its motility; at the same time, use Gram staining, Observe the shape of the cells and measure their size. Electron microscope observation thalline cell flagella situation and taking pictures, the results are shown in Table 1, wherein the photos of the bacterial classification of the...

Embodiment 2

[0099] The production method of the anti-wax bacteria agent with the preservation number CCTCC No: M2015695

[0100] A production method for preparing a wax-clearing and anti-wax liquid bacterial agent with a preservation number of CCTCC No: M2015695, the steps are as follows:

[0101] 1) Slant culture: Inoculate the strain with the preservation number CCTCC No: M2015695 in the slant medium, and culture at 25-28°C for 3 days.

[0102] The formula of the slant medium is: beef extract 0.5%, peptone 1.0%, NaCl 0.5%, agar powder 1.5%-1.8%, the balance is water, pH7.2-7.4.

[0103] 2) Shake flask culture: inoculate the cultured strains on the inclined plane into the liquid medium, the inoculum amount is 5-10%, the rotation speed is 140r / min, cultivate at 37°C for 24 hours, pH6.5-7.5, and the bacterial concentration is 2-7× 10 8 pieces / ml.

[0104] The formula of the liquid medium is: liquid paraffin 2.0%, NH 4 Cl 0.4%, Na 2 HPO 4 0.4%, KH2 PO 4 0.4%, MgSO 4 0.1%, yeast p...

Embodiment 3

[0113] Performance evaluation of the strain agent with deposit number CCTCC No: M2015695

[0114] 1. High temperature resistance

[0115] Inject the bacterial solution into the test tube aseptically, seal it, and treat it at 90°C for 10 days.

[0116] Calculate the number of viable bacteria before and after treatment to obtain the survival rate (%). In the absence of fresh nutrient supply, more than 70% still survive after static treatment at 90°C for 10 days. During the screening process, strains whose survival rate was lower than 70% were eliminated.

[0117] 2. Emulsifying effect on crude oil

[0118] Add 100ml of formation water, a small amount of inorganic salt and 5g of crude oil to a 250ml Erlenmeyer flask, inoculate 10ml of bacterial solution, seal it with a leather stopper, place it in a constant temperature water bath at 65°C for 7 days, and compare it with the blank control to observe the emulsification and dispersion of microorganisms after they act on crude oil...

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Abstract

The invention relates to the technical field of petroleum microbes, and specifically discloses a high-temperature-resistant strong emulsifying microbial strain and its application in wax removal and anti-wax. The Qingfangwax strain disclosed by the present invention is preserved by the China Center for Type Culture Collection, and the preservation number is CCTCC No: M2015695. The microbial bacterial agent disclosed by the invention solves the problems of poor high temperature resistance, poor emulsification effect on crude oil, generally low wax removal rate and high oil recovery cost of the current microbial strains. The wax removal rate of the wax-removing and anti-wax strain disclosed by the invention is as high as 71.4-80.6%, and can grow at 90°C, and the strain agent has a strong emulsifying ability, which can effectively reduce the viscosity and surface tension of crude oil, It prevents the accumulation of wax crystals on the well wall, plays the role of wax prevention and wax removal, and improves the production of oil wells, so it has a wide application value in the actual production of oil fields.

Description

technical field [0001] The invention relates to the technical field of petroleum microbes, and specifically discloses a high-temperature-resistant strong emulsifying microbial strain and its application in removing wax. Background technique [0002] During the crude oil extraction process, the wax in the crude oil is precipitated due to the reduction of pressure and temperature, forming crystals, which adhere to the near wellbore, wellbore and pump valves, etc., affecting the normal operation of the extraction and liquid extraction system. Periodic wax removal operations are required to remove wax crystals attached to the wellbore extraction system. [0003] Microbial oil recovery technology refers to various technologies that use microorganisms and their metabolites to increase crude oil production and recovery. Its application scope involves underground oil displacement, formation plugging removal and viscosity reduction, wellbore wax removal and wax prevention, crude oil...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C09K8/524C12R1/01
CPCC09K8/524C12N1/20C12N1/205C12R2001/01
Inventor 余守德刘如林杜建龙郭彦生郭守泉柴丽敏叶盛军刘伟王芳兰汤蒙云恩龙李超峰杨辉梅万会高新成李旭詹伟
Owner 唐山新新石油科技开发有限公司
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