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Method for promoting growth of Haematococcus pluvialis and accumulation of astaxanthin by flue gas CO2 domestication

A technology of Haematococcus pluvialis and CO2, which is applied in the direction of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of growth inhibition of Haematococcus pluvialis, improve light energy utilization efficiency, and increase growth The effect of increasing the rate and expression

Active Publication Date: 2016-05-18
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when CO 2 The growth of Haematococcus pluvialis was inhibited when the concentration was increased above 10%

Method used

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  • Method for promoting growth of Haematococcus pluvialis and accumulation of astaxanthin by flue gas CO2 domestication

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] (1) Inoculate the Haematococcus pluvialis liquid into 400ml liquid BG-11 medium according to the inoculation amount of 10%, then place the inoculated medium into a 600ml cylindrical photosynthetic reactor, and then transfer it to the photosynthetic reactor High-concentration CO with a volume concentration of 6% 2 Gas, culture for 6 days under the conditions of a gas flow rate of 60ml / min, a light intensity of 3000 Lux and a temperature of 25°C; the above 6% CO 2 The cultured algae strains were transferred to a new liquid BG-11 medium with a 10% inoculum amount, and then a high-concentration CO with a volume concentration of 10% 2 For gas, culture for 6 days under the conditions of gas flow rate of 60ml / min, light intensity of 3000 Lux and temperature of 25℃;

[0035] (2) Take the above 10% CO 2 After 6 days of culture in air, the algae liquid was transferred to a new liquid BG-11 medium at 10% of the inoculum amount, and then the inoculated medium was placed in a cylindrical...

Embodiment 2

[0040] (1) Inoculate the Haematococcus pluvialis liquid into 400ml of liquid BG-11 medium according to the inoculation amount 15%, then place the inoculated medium in a cylindrical photosynthetic reactor with a volume of 600ml, and then transfer it to the photosynthetic reactor High-concentration CO with a volume concentration of 6% 2 Gas, cultured for 5 days under the conditions of a gas flow rate of 60ml / min, a light intensity of 3200 Lux and a temperature of 26℃; the above 6% CO 2 The cultivated algae strains were transferred to a new liquid BG-11 medium with 15% inoculum, and then a high concentration of CO with a volume concentration of 10% 2 Gas, culture for 5 days under the conditions of gas flow rate of 60ml / min, light intensity of 3200 Lux and temperature of 26℃;

[0041] (2) Take the above 10% CO 2 After 5 days of culture in air, the algae liquid was transferred to a new liquid BG-11 medium at 15% of the inoculum amount, and then the inoculated medium was placed in a 600m...

Embodiment 3

[0046] (1) Inoculate the Haematococcus pluvialis liquid into 400ml liquid BG-11 medium according to the inoculation amount 20%, then place the inoculated medium into a cylindrical photosynthetic reactor with a volume of 600ml, and then transfer it to the photosynthetic reactor High-concentration CO with a volume concentration of 6% 2 The gas, the gas flow is 60ml / min, the light intensity is 3500 Lux, and the temperature is 28°C for 4 days; the above 6% CO 2 The cultured algae strains were transferred to a new liquid BG-11 medium with 20% inoculum, and then a high concentration of CO with a volume concentration of 10% 2 For gas, culture for 4 days under the conditions of gas flow rate of 60ml / min, light intensity of 3500 Lux and temperature of 28℃;

[0047] (2) Take the above 10% CO 2 After 4 days of culture in air, the algae liquid was transferred to a new liquid BG-11 medium with 20% of the inoculum, and then the inoculated medium was placed in a 600ml cylindrical photosynthetic r...

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Abstract

The invention relates to a bioactive health product development technique, and aims to provide a method for promoting growth of Haematococcus pluvialis and accumulation of astaxanthin by flue gas CO2 domestication. The method comprises the following steps: carrying out domestication culture on a Haematococcus pluvialis liquid sequentially with 6 vol% high-concentration CO2 gas and 10 vol% high-concentration CO2 gas; carrying out iterative domestication culture by using coal-fired power plant flue gas to obtain a target alga strain; and culturing sequentially under the low illumination intensity of 3000-3500Lux and under the high illumination intensity of 7500-10000Lux, and carrying out centrifugation and freeze-drying on the alga solution to obtain the Haematococcus pluvialis dry alga powder. The method obviously enhances the growth rate and astaxanthin yield, wherein the growth rate is enhanced by 47% as compared with that under air conditions, and the astaxanthin content is enhanced by 25%.

Description

Technical field [0001] The present invention relates to the technical field of the development of biologically active health products, in particular to flue gas CO 2 Domesticate methods to promote the growth of Haematococcus pluvialis and the accumulation of astaxanthin. Background technique [0002] Astaxanthin is currently known as the strongest antioxidant in nature. Its antioxidant capacity is 6000 times that of vitamin C, 1000 times that of vitamin E, 200 times that of tea polyphenols, and 50 times that of grape seed extract. Astaxanthin can enhance the body's anti-aging, anti-inflammatory, anti-ultraviolet and immune functions. Therefore, it is used in various functional products such as health care products, cosmetics, food and feed additives. Among the natural sources of astaxanthin, Haematococcus pluvialis has the highest astaxanthin content, accounting for about 1-5% of its dry weight (MinxiWana, 2014), and the structure of astaxanthin contained in it is required by the...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12P23/00C12R1/89
CPCC12N1/12C12P23/00
Inventor 程军岑可法王智化周俊虎刘建忠黄镇宇周志军杨卫娟张彦威
Owner ZHEJIANG UNIV
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