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Meat duck parvovirus refined yolk antibody

A parvovirus, egg yolk antibody technology, applied in the direction of antiviral immunoglobulin, egg immunoglobulin, peptide, etc., can solve the problems of ineffectiveness and economic loss of duck breeding industry, and achieve the effect of reducing economic loss

Inactive Publication Date: 2016-05-18
CHONGQING SANJIE ZHONGXIN BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Conventional treatment methods are basically ineffective, causing huge economic losses to the duck farming industry

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0020] 1 Preparation of duck parvovirus interleukin 2 adjuvant inactivated vaccine

[0021] 1.1 Antigen preparation

[0022] Meat duck parvovirus, which was isolated from a meat duck farm in Liaocheng, Shandong by the company's researchers in January 2015, was obtained after 12 passages of susceptible duck embryos and identification. Meat duck parvovirus was diluted 20,000 times with normal saline, inoculated into 11-day-old susceptible duck embryos through the allantoic cavity, 0.1ml / embryo, collected the allantoic fluid of dead duck embryos 60-120 hours after inoculation, and passed the sterility test, which is the antigen , stored at -40°C. Simultaneously samples were taken for identification.

[0023] 1.2 Antigen identification

[0024] 1.2.1 Virus content

[0025] The antigen was serially diluted 10 times with normal saline, and 10 -5 、10 -6 and 10 -7 Inoculate 9-day-old susceptible duck embryos at 3 dilutions, inoculate 5 eggs for each dilution, 0.1ml / embryo, seal...

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Abstract

The invention discloses a meat duck parvovirus refined yolk antibody and relates to the field of antibodies. The scheme is provided for solving the problem that existing meat duck parvoviruses cannot be effectively prevented and treated and mainly comprises vaccine preparation, a vaccine immunity procedure and yolk antibody extraction so as to achieve a function of preventing and treating the meat duck parvoviruses.

Description

technical field [0001] The invention relates to a refined egg yolk antibody of duck parvovirus, and mainly relates to the field of antibodies. Background technique [0002] Since November 2014, meat ducks have been prevalent in some areas of my country. The clinical symptoms are duck bill dysplasia, tongue exposure, swelling, and downward bending. The incidence rate is 10-30%. The age of duck onset is concentrated in 2 stages. The first stage is 3-13 days old, the symptoms are unstable standing, webbed valgus, limp or rolling when forced to drive; the second stage is 21-28 days old, mainly movement disorders, unable to keep up with large groups, squatting lying on the side or lying on the side, and showing symptoms of tongue exposure. The feed-to-meat ratio of sick ducks is more than 30% higher than that of normal ducks. characteristic disease. Poultry disease expert Diao Youxiang and others were the first to isolate the pathogen. The gene sequence identification has more tha...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K16/08C07K16/02
CPCC07K16/02C07K16/081
Inventor 魏思远赵光伟
Owner CHONGQING SANJIE ZHONGXIN BIOLOGICAL ENG CO LTD
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