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Composition for 3D printed cartilage bracket, microsphere and preparation method of composition

A 3D printing and composition technology, applied in tissue regeneration, medical science, prosthesis, etc., can solve the problems of central nervous system toxicity, strong spasm, life-threatening, etc., and achieve the promotion of cartilage tissue formation, good biocompatibility, The effect of suitable degradation rate

Active Publication Date: 2016-05-04
XIANGTAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The role of strychnine in promoting the proliferation of chondrocytes is encouraging, but at the same time strychnine and strychnine also have severe central nervous system toxicity, and because the therapeutic dose of strychnine is close to the toxic dose, it is a little careless It can cause convulsions, and even strong convulsions and convulsions can be life-threatening, thus greatly limiting its clinical use

Method used

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  • Composition for 3D printed cartilage bracket, microsphere and preparation method of composition
  • Composition for 3D printed cartilage bracket, microsphere and preparation method of composition
  • Composition for 3D printed cartilage bracket, microsphere and preparation method of composition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Put 1 mg of total strychnine alkaloids (also known as strychnine total alkaloids, wherein the content of strychnine is 0.512 mg, and the content of strychnine is 0.453 mg) and 1 mL of ethanol are placed in a 2 mL EP tube , ultrasonic vibration makes it dissolve, obtains the solution 1 that contains the total alkaloid of strychnium, for subsequent use;

[0057] Put 2 mg of PLGA solid in a 2 mL EP tube, add 1 mL of dichloromethane to dissolve it completely, and obtain a dichloromethane solution 2 containing polylactic-glycolic acid, and set aside;

[0058] Place 1 mg of chitosan in 20 mg of 1% by weight PVA aqueous solution, and ultrasonically vibrate to dissolve it to obtain solution IV for subsequent use;

[0059] (1) Mix 100 microliters of solution 1 with all of solution 2 at minus 4°C and keep for 20 minutes to obtain solution I;

[0060] (2) Mix solution I, which is ultrasonicated at minus 4°C for 5 minutes (power is 5W), and 20 mg of 1% by weight PVA aqueous soluti...

Embodiment 2

[0070] Put 0.8mg of strychnine and 1mL of ethanol in a 2mL EP tube, and dissolve them by ultrasonic vibration to obtain solution 1 containing strychnine, which is set aside;

[0071] Put 2.5 mg of PLGA solid in a 2 mL EP tube, add 1 mL of dichloromethane to dissolve it completely, and obtain a dichloromethane solution 2 containing polylactic-glycolic acid, and set aside;

[0072] Place 1.2 mg of chitosan in 25 mg of 1% by weight PVA aqueous solution, and ultrasonically vibrate to dissolve it to obtain solution IV for subsequent use;

[0073] (1) Mix 100 microliters of solution 1 with all of solution 2 at minus 8°C and keep for 20 minutes to obtain solution I;

[0074] (2) Mix solution I, which was ultrasonicated at minus 8°C for 10 minutes (power is 5W), with 20 mg of 1% by weight PVA aqueous solution II at minus 8°C, and ultrasonicate for 80 minutes (power is 8W), to obtain solution III;

[0075] (3) The solution III was contacted with the solution IV for 3.5 hours under sti...

Embodiment 3

[0084] Put 0.4mg of strychnine and 0.3mg of strychnine and 1mL of ethanol in a 2mL EP tube, and dissolve them by ultrasonic vibration to obtain a solution 1 containing strychnine and strychnine, which is set aside;

[0085] Put 1.8mg of PLGA solid into a 2mL EP tube, add 1mL of dichloromethane to dissolve it completely, and obtain a dichloromethane solution 2 containing polylactic-glycolic acid, and set aside;

[0086] Place 0.93 mg of chitosan in 20 mg of 1% by weight of PVA aqueous solution, and dissolve it with ultrasonic vibration to obtain solution IV for subsequent use;

[0087] (1) Mix 100 microliters of solution 1 with all of solution 2 at 0°C and keep for 30 minutes to obtain solution I;

[0088] (2) Mix solution I, which was sonicated at 0°C for 10 minutes (power is 5W), with 18 mg of 1% by weight PVA aqueous solution II at 0°C and sonicate for 100 minutes (power is 5W), to obtain solution III;

[0089] (3) The solution III was contacted with the solution IV for 3 h...

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Abstract

The invention relates to the field of medicine, and discloses composition for a 3D printed cartilage bracket, a microsphere containing the composition and a preparation method of the composition. The composition comprises polylactic-co-glycolic acid, brucine, polyvinyl alcohol, chitosan and optional strychnine. Taking the total weight of the composition as the baseline, the total content of brucine and optional strychnine is 0.1-10 wt%. The composition for the 3D printed cartilage bracket can be taken as raw materials of the 3D printed cartilage bracket and has appropriate degradation speed; degradation products are harmless to human bodies, good biocompatibility is achieved, adhesion and proliferation of cells are facilitated, and cartilage tissue is promoted to form; besides, a slow-release medicine function is achieved, and the composition has no adverse influence on efficacy.

Description

technical field [0001] The invention relates to the field of medicines, in particular to a composition for 3D printing cartilage scaffolds, a microsphere for 3D printing cartilage scaffolds and a preparation method thereof. Background technique [0002] Controlled release is a technology that embeds drugs or other active substances in polymer materials and releases them into the surrounding environment at a certain rate by means of diffusion. Compared with traditional drug delivery modes, controlled release of drugs can improve drug therapy. In addition to the accuracy, safety and effectiveness of the drug, it can also significantly reduce the adverse reactions of the drug. The drug carrier is an important part of the drug sustained release system and is also the main factor affecting the efficacy of the drug. Poly(lactic-co-glycolic acid), PLGA is a degradable polymer organic compound, which has the advantages of good biocompatibility, safety and targeted modification. Aft...

Claims

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Application Information

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IPC IPC(8): A61L27/20A61L27/18A61L27/16A61L27/54C08L67/04C08L29/04C08L5/08C08K5/3437
CPCA61L27/16A61L27/18A61L27/20A61L27/54A61L2300/204A61L2300/412A61L2430/06C08L67/04C08L2203/02C08L2205/03C08L29/04C08L5/08C08K5/3437
Inventor 吴萍帅词俊杨春光严建业梁琴
Owner XIANGTAN UNIV
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