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Detection method for antibiotic residues in meat products

A technology for meat food and antibiotics, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of low accuracy of test results and extraction of antibiotic components, etc., to improve the dissolution rate, ensure accuracy, and improve accuracy Effect

Active Publication Date: 2016-04-27
ZHONGBIAO TESTING HENAN SERVICE +1
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The use of conventional chemical reagents cannot completely extract the antibiotic components, resulting in low accuracy of the test results

Method used

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  • Detection method for antibiotic residues in meat products
  • Detection method for antibiotic residues in meat products
  • Detection method for antibiotic residues in meat products

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Embodiment Construction

[0013] The method for detecting antibiotic residues in meat foods of the present invention will be described in detail below through specific examples. Those skilled in the art should understand that the embodiments described below are only exemplary descriptions of the present invention, and are not used to impose any restrictions on it.

[0014] Take the pork containing antibiotics after preliminary screening (such as the microbial inhibition test paper method) as a sample to test its 8 kinds of macrolide antibiotics (spiramycin, azithromycin, tilmicosin, elixiromycin, erythromycin) , Tylosin, clarithromycin, roxithromycin), to introduce the implementation process and effects of the present invention.

[0015] 1. Preparation of sample solution A

[0016] 1.1 Extraction steps

[0017] Use a pulverizer to prepare a homogenate of the pork to be tested, weigh 5g of the homogenate into a centrifuge tube, add 1g of polyoxyethylene polyoxypropylene polyether, ultrasonically shake for 30 m...

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Abstract

The invention provides a detection method for antibiotic residues in meat products. The detection method comprises the following steps: (1) preparing homogenate from a to-be-measured meat product by using a pulverizer, placing the homogenate in a centrifuge tube, successively adding de-emulsifier, hexane and a phosphate buffer solution and carrying out ultrasonic extraction and centrifugation so as to obtain to-be-purified liquid; (2) transferring the to-be-purified liquid into a solid-phase extractioncolumn, carrying out purifying, collecting eluate, blowing the eluate with nitrogen in a water bath until the eluate is nearly dry, adding an aqueous acetonitrile solution, mixing the eluate with the aqueous acetonitrile solution in a turbine mixer, carrying out filtration with a microfiltration membrane and standing obtained filtrate for subsequent measurement; and (3) carrying out determination and affirmation by using liquid chromatography-tandem mass spectrometry and carrying out quantification by using an external standard method. The detection method for antibiotic residues in meat products can effectively improve accuracy of detection results and effectively guarantees food safety.

Description

Technical field [0001] The invention relates to a method for detecting antibiotic residues in meat foods. Background technique [0002] Antibiotics are widely used to treat a variety of bacterial infections in animals. Antibiotic drugs enter the animal's body mainly through injection, oral administration, and drinking. Excess antibiotics will remain in the muscles and milk at the injection site before the end of the drug withdrawal period. When veterinarians use different antibiotics together in clinical medication, it is also easy to cause antibiotics to remain in the animal's body, leading to meat contamination. Some antibiotics are also used as feed additives to prevent animal bacterial diseases and promote animal growth for a long time. For example, oxytetracycline additives and chlortetracycline additives enter the animal's body through the oral cavity, and cannot be completely excreted in a short time, and are easily accumulated in the body, causing antibiotic drug resid...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06
CPCG01N30/06G01N30/88G01N2030/062
Inventor 赵林萍常伟鲁龙苗银萍曾小宇李慧英王盼
Owner ZHONGBIAO TESTING HENAN SERVICE
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