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Broccoli polypeptide extract as well as preparation method and application thereof

A technology of broccoli and extract, applied in the field of broccoli polypeptide extract, can solve the problems such as few literature reports, and achieve the effects of simple equipment, high yield and improved yield

Active Publication Date: 2016-04-27
ZHEJIANG ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Immunity enhancement has become a health care issue that people pay more and more attention to today, but there are few literature reports on the isolation of broccoli peptides to enhance immunity

Method used

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  • Broccoli polypeptide extract as well as preparation method and application thereof
  • Broccoli polypeptide extract as well as preparation method and application thereof
  • Broccoli polypeptide extract as well as preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation of embodiment 1 broccoli polypeptide extract

[0036] (1) Take broccoli stems and leaves, first carry out enzymatic hydrolysis pretreatment, use pectinase (Novozymes (China) Biotechnology Co., Ltd.) and cellulase (Novozymes (China) Biotechnology Co., Ltd.) Compound at a ratio of 1:1 to form a compound enzyme, then add water to form an enzymolysis solution, the ratio of the mass of the compound enzyme to the volume of the enzymolysis solution is 2.5g: 100mL, that is, the total amount of enzyme added is 2.5% (w / v), Put the broccoli stems and leaves into the enzymolysis solution for enzymolysis, the enzymolysis temperature is 60°C, the enzymolysis time is 3h, then the pH is adjusted to 8-8.5 for alkaline extraction, the alkaline extraction time is 1.5h, and then centrifuged at 5000r / min for 15min to adjust the The pH of the supernatant was at the isoelectric point pI to precipitate protein, discard the supernatant, wash the precipitate three times with wate...

Embodiment 2

[0039] The preparation of embodiment 2 broccoli polypeptide extract

[0040] (1) Take broccoli stems and leaves, first carry out enzymatic hydrolysis pretreatment, use pectinase (Novozymes (China) Biotechnology Co., Ltd.) and cellulase (Novozymes (China) Biotechnology Co., Ltd.) Compound at a ratio of 0.5:1 to form a compound enzyme, then add water to form an enzymolysis solution, the ratio of the mass of the compound enzyme to the volume of the enzymolysis solution is 1g: 100mL, that is, the total amount of enzyme added is 1% (w / v), and the Put broccoli stems and leaves into enzymolysis solution for enzymolysis, enzymolysis temperature 50℃, enzymolysis time 4h, then adjust pH 9.0 for alkaline extraction, alkaline extraction time 1h, then centrifuge at 4000r / min for 10min, adjust the supernatant The pH is at the isoelectric point pI to precipitate the protein, discard the supernatant, wash the precipitate three times with water, adjust the pH to 7.0, and dry to obtain broccoli...

Embodiment 3

[0043] The preparation of embodiment 3 broccoli polypeptide extract

[0044] (1) Take broccoli stems and leaves, first carry out enzymatic hydrolysis pretreatment, use pectinase (Novozymes (China) Biotechnology Co., Ltd.) and cellulase (Novozymes (China) Biotechnology Co., Ltd.) Compound at a ratio of 1.5:1 to form a compound enzyme, then add water to form an enzymolysis solution, the ratio of the mass of the compound enzyme to the volume of the enzymolysis solution is 5g: 100mL, that is, the total amount of enzyme added is 5% (w / v), and the Put broccoli stems and leaves into enzymolysis solution for enzymolysis, enzymolysis temperature 70°C, enzymolysis time 2h, then adjust pH 7.5 for alkaline extraction, alkaline extraction time 2h, then centrifuge at 6000r / min for 20min, adjust the supernatant The pH is at the isoelectric point pI to precipitate the protein, discard the supernatant, wash the precipitate three times with water, adjust the pH to 7.0, and dry to obtain broccol...

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Abstract

The invention discloses a broccoli polypeptide extract as well as preparation method and application thereof. The preparation method comprises the following steps: preparing stems and leaves of broccoli, firstly enzymatically hydrolyzing the stems and the leaves by virtue of complex enzyme, then conducting alkaline extraction, and separating, precipitating, washing and drying so as to obtain broccoli protein powder; adding water, then adding alkaline protease and flavor protease for further enzymatic hydrolysis under the following conditions: temperature is 45-60 DEG C and pH value is 8.5-10, inactivating the enzyme, cooling, centrifuging, conducting rotary evaporation, concentrating and freeze-drying so as to obtain enzymatically hydrolyzed broccoli polypeptide; and ultra-filtering the enzymatically hydrolyzed broccoli polypeptide by virtue of a filter membrane and separating the enzymatically hydrolyzed broccoli polypeptide by virtue of a dextran gel column, eluting by taking a phosphate water solution as an eluent, collecting the eluent, filtering, evaporating to dryness, collecting and drying, so that the broccoli polypeptide extract is obtained. The broccoli polypeptide extract disclosed by the invention, which is prepared through a special preparation method, is significant in effect on enhancing immunity; therefore, the broccoli polypeptide extract can be used for preparing health care food and medicines capable of enhancing the immunity.

Description

technical field [0001] The invention relates to the field of broccoli polypeptide extract, in particular to a broccoli polypeptide extract, a preparation method thereof, and an application in preparing immunity-enhancing health food and medicine. Background technique [0002] Broccoli (Brassica oleracea L.var.botrytis L), also known as broccoli, is a biennial plant of the genus Brassica in the family Brassicaceae. Tumor active substances and polyphenols, flavonoids and other antioxidant active substances, and the protein and amino acid content are also high, with very high edible and medicinal health care value. [0003] Studies abroad have proved that sulforaphane has the function of improving immunity. The American "Nutrition" magazine also published research results that broccoli can effectively prevent prostate cancer. ShapiroTA et al compared the safety, tolerance and metabolism in vivo of glucosinolate, glucoraphanin and sulforaphane isothiocyanate in broccoli bud ex...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/36C07K1/34C07K1/16A61K38/01A61P37/04A23L33/18A23L33/185
CPCA61K38/01C07K1/16C07K1/34C07K1/36C12P21/06
Inventor 党亚丽么春艳俞焕腾王永东
Owner ZHEJIANG ACAD OF MEDICAL SCI
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