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Bacillus coagulans having aquatic pathogen antagonistic characteristic and application of bacillus coagulans

A technology of bacillus coagulans and aquatic pathogens, applied in the field of microorganisms, can solve the problems of difficult preservation, easy inactivation, poor stress resistance, etc., and achieve the effect of reducing pollutant content and improving the quality of aquaculture water

Active Publication Date: 2016-04-27
江苏省苏微微生物研究有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, common lactic acid bacteria do not form spores during the growth process, have poor stress resistance, are easily inactivated, and are difficult to preserve, so their application in the feed industry is limited.
There is no patent report on this aspect of Bacillus coagulans

Method used

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  • Bacillus coagulans having aquatic pathogen antagonistic characteristic and application of bacillus coagulans
  • Bacillus coagulans having aquatic pathogen antagonistic characteristic and application of bacillus coagulans
  • Bacillus coagulans having aquatic pathogen antagonistic characteristic and application of bacillus coagulans

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Embodiment 1

[0024] Embodiment 1: Screening and isolation of strains

[0025] Allogenetic gibel carp samples were collected in Wuxi Goose Lake breeding pond, the fish body was dissected to take the intestinal tract, added physiological saline, and ground to make a homogenate, each sample was absorbed 1.0mL, heated at 80°C for 10min, and pipette gun to absorb 0.1mL Spread on YPD solid medium and culture at 37°C. According to the size of the colony, the well-growing colony was selected and screened repeatedly until a uniform single colony was obtained, which was named JSSW-LA-07-1.

Embodiment 2

[0026] Embodiment 2: strain identification

[0027] (1) Morphological characteristics

[0028] Strain JSSW-LA-07-1, Gram staining was positive, microscopic examination was rod-shaped, cell diameter <1 μm, and could form spores. The diameter of the colony on the MRS plate is 2.0-3.0mm, the shape is irregular, the surface is wrinkled, opaque, and no pigment. A large number of round, wrinkled, opaque, white colonies can be obtained on LB medium.

[0029] (2) Biochemical properties

[0030] Strain JSSW-LA-07-1 is positive for Gram staining, catalase, β-galactosidase, VP test and methyl red test, and can grow at 50°C. Oxidase, urease, arginine dihydrolase, ornithine decarboxylase, lysine decarboxylase tests are negative, can not hydrolyze starch, gelatin, casein, and do not produce indole and H 2 S, cannot grow on citric acid.

[0031] The results of growth experiments showed that the strain could utilize D-cellobiose, α-D-glucose, D-mannose, D-fructose, D-trehalose, dextrin, ...

Embodiment 3

[0039] Embodiment 3: Bacteriostasis determination

[0040] Place a piece of sterile filter paper at a concentration of 1.0×10 7 CFU / mL Bacillus coagulans JSSW-LA-07-1 soaked in fresh culture solution for 1.0h. Take the concentration as 5.0×10 6 Common pathogenic bacteria in aquaculture in CFU / mL Aeromonas hydrophila, Aeromonas sobria, Aeromonas victoria, Aeromonas caviae, Listonia eelensis, Edwardsiella parahaemolyticus Vibrio vibrio, Escherichia coli, Vibrio alginolyticus liquid culture solution 0.1mL, spread on the LB agar medium plate respectively, and then paste the filter paper soaked in the bacterial solution on the petri dish, stick 3 pieces on each plate , and each plate was replicated 3 times. The plates were placed in a 28°C incubator for 24 and 48 hours, and the size of the inhibition zone was measured. The measurement results are shown in Table 1:

[0041] Table 1 Bacillus coagulans JSSW-LA-07-1 antibacterial assay results (inhibition zone diameter: mm)

[00...

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Abstract

The invention discloses bacillus coagulans having an aquatic pathogen antagonistic characteristic and application of the bacillus coagulans, and belongs to the technical field of microorganisms. The strain is classified and named as bacillus coagulans JSSW-LA-07-1 and is preserved in China General Microbiological Culture Collection Center with preservation number of CGMCC No. 10182. The invention also discloses a preparation method of a strain powder preparation, wherein the method comprises the following steps: activating and fermentation-cultivating the bacillus coagulans JSSW-LA-07-1 which serves as an initial strain, so that a fermentation broth is obtained, centrifuging the fermentation broth at a high speed and collecting a wet strain; mixing the wet strain with dry starch in the mass ratio of 1 to (2-10); drying at 30-50 DEG C for 20-24h; grinding by virtue of a grinder; and filtering by virtue of a 0.9mm sieve, so that the strain powder preparation is obtained; in the strain powder preparation, bacterium concentration is no less than 5.0 to the power of 8 (CFU) / g; the strain powder preparation, when used as an additive of fish culture feed, is added by 1.0-5.0mg / kg to feed basal diet; and the strain powder preparation, when used as a water quality improver for aquaculture, is sprayed to a full tank by the dosage of 50-200g / mu.m (mu is a unit of area, equal to 0.0667 hectares).

Description

technical field [0001] The invention relates to a bacillus coagulans with antagonistic properties of aquatic pathogens and an application thereof, belonging to the technical field of microbes. Background technique [0002] In recent years, my country's aquaculture industry has developed vigorously, which has greatly enriched people's material life. However, with the increasing scale of high-density intensive aquaculture, the ecological environment of aquaculture water is deteriorating, which greatly increases the chance of cross-infection of pathogens among aquatic animals, resulting in frequent occurrence of various fish diseases, and even large-scale death of aquatic animals. For the prevention and treatment of fish diseases, the traditional method is to use antibiotics, which may have a better effect in the short term, but long-term use will easily lead to stronger drug resistance of pathogenic bacteria, imbalance of intestinal flora in aquatic animals, and long-term en...

Claims

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Application Information

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IPC IPC(8): C12N1/20A23K1/16A23K1/18C12R1/07
Inventor 孙梅陈秋红张维娜夏飞高亮施大林匡群张宪中邹三元
Owner 江苏省苏微微生物研究有限公司
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