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Polypeptide bioactive coating and biomaterial as well as preparation method and application of biomaterial

A technology of biological activity and biomaterials, applied in the field of biomaterials and their preparation, and polypeptide bioactive coatings, can solve problems such as limited development and complexity, and achieve the effect of promoting cell adhesion, good cell adhesion, and promoting autoimmune function

Inactive Publication Date: 2016-04-20
HUBEI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, LB deposition requires complex equipment and is only suitable for amphiphilic molecules, and SAM is only suitable for a few specific molecules interacting with the base, these shortcomings limit the development of these two technologies.

Method used

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  • Polypeptide bioactive coating and biomaterial as well as preparation method and application of biomaterial
  • Polypeptide bioactive coating and biomaterial as well as preparation method and application of biomaterial
  • Polypeptide bioactive coating and biomaterial as well as preparation method and application of biomaterial

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] Prepare the PLL-PLGA antimicrobial coating as follows:

[0053] Use a stainless steel sheet as the base material, after ultrasonic cleaning in 70% alcohol aqueous solution, immerse it in 1mg / ml PLL Glycine-NaOH (50mM, pH=10) buffer solution for 15 minutes, and clean it in Glycine-NaOH solution Afterwards, the air was dried; the specimen was immersed in the Glycine-NaOH (50mM, pH=10) buffer solution of 1mg / ml PLGA for 15 minutes, and the air was dried after cleaning; the above steps were repeated 20 times to obtain 20 double-layered PLL / PLGA coating: immerse the sample piece in a PBS solution of cephalosporin sodium (5 mg / ml) for 5 minutes to obtain an antibiotic-loaded polypeptide coating.

[0054] Coating in the present embodiment is carried out ultraviolet-visible light absorption measurement:

[0055] Such as figure 1 As shown, for the determination, quartz glass is used as the material, and the quartz plate sample is placed in a UV-visible spectrophotometer to mea...

Embodiment 2

[0061] Follow the steps below to prepare polypeptide antibacterial and bioactive coatings

[0062] After the titanium alloy metal sheet is ultrasonically cleaned in 70% alcohol aqueous solution, it is immersed in the Tris-NaAc (10mM, pH=4.0) buffer solution of 1mg / ml PLL for 10 minutes, and the air is dried after cleaning in the solution; Immerse in the Tris-NaAc buffer solution of 0.5mg / ml Arg-Gly-Asp (RGD) for 15 minutes, air dry after cleaning; immerse the sample piece in the Tris-NaAc buffer solution of 1mg / ml PLGA for 15 minutes, Air-dry after cleaning; Repeat above-mentioned steps 20 times, obtain the PLL / RGD / PLGA coating that has; Sample piece is immersed in the PBS solution of gentamicin (5mg / ml) and TGF-β (0.5ug / ml) 5 Minutes, after rinsing and drying, a polypeptide antibacterial and bioactive coating is obtained.

[0063] The coating in this embodiment is subjected to ultraviolet spectrum analysis, wherein the RGD layer is labeled with fluorescein isothiocyanate (FI...

Embodiment 3

[0075] Prepare the PLL-PLGA antimicrobial coating as follows:

[0076] Use a stainless steel sheet as the base material, after ultrasonic cleaning in 70% alcohol aqueous solution, immerse it in 0.5mg / ml PLL Glycine-NaOH (50mM, pH=12) buffer solution for 20 minutes, in Glycine-NaOH solution After cleaning, the air was blown dry; the samples were immersed in the Glycine-NaOH (50mM, pH=12) buffer solution of 0.5mg / ml PLGA for 20 minutes, and the air was blown dry after cleaning; the above steps were repeated 20 times to obtain 20 double The PLL / PLGA coating of layer; The specimen piece is immersed in the PBS solution of cephalosporin sodium (5mg / ml) 5 minutes, obtains the polypeptide coating of loading antibiotic.

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PUM

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Abstract

The invention provides a polypeptide bioactive coating, which mainly comprises a plurality of polypeptide film layers and an active material layer, wherein two adjacent polypeptide film layers have different charges; two adjacent polypeptide films are combined through charge acting force; each polypeptide film is 3-10nm in thickness; a nano coating material of the polypeptide bioactive coating is polypeptide with good biocompatibility, not only meets the characteristics of being nontoxic, free of thrill, and free of carcinogenesis, teratogenesis and mutagenesis, but also has good bacteriostatic and antibacterial effects, has biological activity and is capable of promoting cell adhesion, growth and proliferation. The invention further provides a biomaterial, which comprises a base material and the bioactive coating loaded on the surface of the base material. The invention further provides a method for preparing the biomaterial. The invention further provides an application of the biomaterial in a surgical operation; and preferably, the surgical operation is an orthopedic operation.

Description

technical field [0001] The invention relates to the field of biochemistry, in particular to a polypeptide biologically active coating, a biological material and a preparation method and application thereof. Background technique [0002] The progress of modern medicine is inseparable from the development of biomaterials. From plastic surgery, cardiovascular implantation stents, human orthopedic replacement and repair, biological tissue engineering to drug delivery systems, medical biomaterials are used in various fields of biomedicine [1]. Implantation of these materials into the human body often causes local and systemic adverse reactions. As a biomaterial, it must meet the following requirements: non-toxic, non-irritating, and non-toxic, and have good compatibility with cells and tissues. The host reaction caused by the material is microscopically caused by the host cell's recognition of the surface, so the properties of the biological surface often determine whether the m...

Claims

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Application Information

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IPC IPC(8): A61L27/34A61L27/54A61L31/10A61L31/16
CPCA61L27/34A61L27/54A61L31/10A61L31/16A61L2300/214A61L2300/404A61L2300/406A61L2300/426A61L2300/606A61L2400/18A61L2420/08C08L77/04
Inventor 江兵兵陈学琴
Owner HUBEI UNIV
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