Algicide and preparation method and application thereof
A technology of algaecide and methanol, applied in the direction of herbicides and algicides, botany equipment and methods, applications, etc., can solve the problems of uncertainty in the effect of pathogenic bacteria, high cost, and impact on the ecosystem
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Embodiment 1
[0020] 1. Weigh 5g of dried grape leaves, grind them thoroughly, add 50ml of 50% methanol to extract at room temperature and in the dark for 48h, the concentration of the extract is 100mg / ml;
[0021] 2. Measure 40ml of the methanol extract, distill the methanol extract at 64.5°C until the methanol is evaporated, and add water to make up to 40ml.
[0022] 3. Add the distillate to a cell density of 4×10 6 Chlamydomonas solution, so that the final concentration of 12mg / ml. After 12 hours of treatment, the inhibition rate reached 59% compared with the control; after 48 hours, all algae cells died. Example 2
Embodiment 2
[0023] 1. Weigh 20g of dried grape leaves, grind them thoroughly, add 100ml of 50% methanol to extract at room temperature and in the dark for 48 hours, the concentration of the extract is 200mg / ml;
[0024] 2. Measure 50ml of the methanol extract, distill the methanol extract until the methanol is evaporated, and add water to make up to 100ml.
[0025] 3. Add the distillate to a cell density of 4×10 6 cyanobacteria solution, so that the final concentration of 20mg / ml. After 12 hours of treatment, the inhibition rate reached 53% compared with the control; after 48 hours, all algae cells died. Example 3
Embodiment 3
[0026] 1. Weigh 5g of dried grape leaves, grind them thoroughly, add 50ml of 50% methanol to extract at room temperature and in the dark for 48h, the concentration of the extract is 100mg / ml;
[0027] 2. Measure 40ml of the methanol extract, distill the methanol extract until the methanol is evaporated, and add water to make up to 40ml.
[0028] 3. Add the distillate to a cell density of 4×10 6 In the chlorella solution, the final concentration is 15mg / ml. After 12 hours of treatment, the inhibition rate reached 55% compared with the control; after 48 hours, all algae cells died.
[0029] The distillate of embodiment 1 is added to cell density and is 4 * 10 6 Chlamydomonas solution, and make its concentration 12mg / ml, respectively in the treatment of 12h, 24h and 48h to measure the growth of the cells. Adding an equal volume of double distilled water was used as a control.
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