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Algicide and preparation method and application thereof

A technology of algaecide and methanol, applied in the direction of herbicides and algicides, botany equipment and methods, applications, etc., can solve the problems of uncertainty in the effect of pathogenic bacteria, high cost, and impact on the ecosystem

Inactive Publication Date: 2016-04-20
ZHEJIANG FORESTRY UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The physical method is to manually remove the algae on the water surface, which cannot fundamentally solve the effect of the enriched nutrients stimulating the growth of algae, and requires a lot of manpower and material resources for repeated removal, and the cost is high; chemical algicides have The time is short, the effect is quick, and the effect of quickly killing algae can be achieved, but the current chemical algae removal drugs will be enriched and amplified in the organism, thereby seriously affecting the ecosystem; biological algae removal is mainly carried out by algae pathogenic microorganisms , but the mutation of pathogenic bacteria under natural conditions will have a great impact on other biological groups, and there is great uncertainty in the effect of pathogenic bacteria

Method used

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  • Algicide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] 1. Weigh 5g of dried grape leaves, grind them thoroughly, add 50ml of 50% methanol to extract at room temperature and in the dark for 48h, the concentration of the extract is 100mg / ml;

[0021] 2. Measure 40ml of the methanol extract, distill the methanol extract at 64.5°C until the methanol is evaporated, and add water to make up to 40ml.

[0022] 3. Add the distillate to a cell density of 4×10 6 Chlamydomonas solution, so that the final concentration of 12mg / ml. After 12 hours of treatment, the inhibition rate reached 59% compared with the control; after 48 hours, all algae cells died. Example 2

Embodiment 2

[0023] 1. Weigh 20g of dried grape leaves, grind them thoroughly, add 100ml of 50% methanol to extract at room temperature and in the dark for 48 hours, the concentration of the extract is 200mg / ml;

[0024] 2. Measure 50ml of the methanol extract, distill the methanol extract until the methanol is evaporated, and add water to make up to 100ml.

[0025] 3. Add the distillate to a cell density of 4×10 6 cyanobacteria solution, so that the final concentration of 20mg / ml. After 12 hours of treatment, the inhibition rate reached 53% compared with the control; after 48 hours, all algae cells died. Example 3

Embodiment 3

[0026] 1. Weigh 5g of dried grape leaves, grind them thoroughly, add 50ml of 50% methanol to extract at room temperature and in the dark for 48h, the concentration of the extract is 100mg / ml;

[0027] 2. Measure 40ml of the methanol extract, distill the methanol extract until the methanol is evaporated, and add water to make up to 40ml.

[0028] 3. Add the distillate to a cell density of 4×10 6 In the chlorella solution, the final concentration is 15mg / ml. After 12 hours of treatment, the inhibition rate reached 55% compared with the control; after 48 hours, all algae cells died.

[0029] The distillate of embodiment 1 is added to cell density and is 4 * 10 6 Chlamydomonas solution, and make its concentration 12mg / ml, respectively in the treatment of 12h, 24h and 48h to measure the growth of the cells. Adding an equal volume of double distilled water was used as a control.

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Abstract

The invention discloses an algicide. The algicide takes a methanol extract of grape leaves as an active ingredient. According to the algicide, biological ingredients in the grape leaves are fully utilized, and the growth of ordinary algae cells can be inhibited obviously. According to the technical scheme, grape leaves abandoned after trimming grapes are utilized, so that environment pollution is avoided, and excellent ecological and economic benefits are obtained.

Description

technical field [0001] The invention relates to an algaecide, which belongs to the field of algae removal and control. Background technique [0002] A large amount of domestic sewage and industrial wastewater rich in N and P are discharged into rivers and lakes, and then collected into the ocean, causing eutrophication of the water body, causing algae and other plankton to multiply rapidly and causing serious environmental disasters. It occurs frequently in various lakes, rivers and sea areas in my country. The massive reproduction of algae not only seriously hinders the water surface traffic, but also seriously damages the water body environment and endangers the safety of the ecological environment. On the surface of the lake, the sunlight is blocked, so that the underwater organisms cannot get enough sunlight, which affects their survival and reproduction; (3) The algae secretes or produces mucus, which adheres to the gills of aquatic animals such as fish, hinders breathi...

Claims

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Application Information

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IPC IPC(8): A01N65/08A01P13/00
CPCA01N65/08
Inventor 左照江
Owner ZHEJIANG FORESTRY UNIVERSITY
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