Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Printing method for biochip and its application

A printing method and biochip technology, applied in organic compound libraries, series/parallel reactions, chemical libraries, etc., can solve problems such as the influence of membrane materials and the influence of membranes on the adsorption and immobilization of probes and detection performance

Active Publication Date: 2018-06-29
杨华卫
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The problem of ink printing is that the ink itself contains a variety of complex physical and chemical substances, which may inhibit the proteins and enzymes used in protein chips and the color-developing enzymes used in gene chips; At this time, the high temperature generated by the printing equipment may have an impact on the membrane material, thereby affecting the adsorption and fixation of the membrane to the probe and its subsequent detection performance.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Printing method for biochip and its application
  • Printing method for biochip and its application
  • Printing method for biochip and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0098] Example 1: Using a bioprinting method to prepare a bioprinting HPV gene chip for detecting HPV16 samples

[0099] 1. Using the bioprinting method of the present invention to prepare bioprinting HPV gene chips

[0100] (1) Prepare marker solution and probe solution

[0101] Prepare hpv6 probes (positive control probes, markers), negative probes, HPV16 probes, hpv18 probes, hpv31 probes, hpv33 probes, hpv35 probes and hpv39 probe solutions. The concentration of the probe solutions are all It is 2μM.

[0102] (2) Use the above probe solution to prepare a bioprinted HPV gene chip

[0103] Using nitrocellulose membrane as the chip carrier, using the Boao PresonalAssayer16 sampler setting program, print the hpv6 probe (positive control probe, marker) solution on the nitrocellulose membrane of the gene chip substrate into different segments The lines of the area; at the same time, the negative control probe and the specific biological probe of the sample to be tested are printed on th...

Embodiment 2

[0116] Example 2: Using a bioprinting method to prepare a bioprinted protein chip for detecting TOX antibody samples

[0117] 1. Using bio-printing method to prepare bio-printed protein chip

[0118] (1) Prepare marker solution and probe solution

[0119] Prepare positive control probe (marker), negative control probe, purified antigen TOX probe, RV probe, CMV probe and HSV-II probe solution respectively, the concentration of the probe solution is 2μM.

[0120] (2) Use the above probe solution to prepare a bio-printed protein chip

[0121] Print the positive control probes on the substrate of the protein chip as lines that separate different areas; at the same time, print the negative control probes and specific biological probes of the sample to be tested on the substrate of the same gene chip in the form of dots , Including purified antigen TOX probe, RV probe, CMV probe and HSV-II probe; make the dots printed by these probes exist in the area divided by the positive control probe (m...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention relates to a printing method of a biological chip and an application of the biological chip. The printing method comprises steps as follows: a to-be-detected sample and a marker or a to-be-detected sample, a marker and a biological molecule with a quality control function on the to-be-detected sample are fixed on a substrate of the biological chip in a graphic mode, and the biological chip is prepared. According to the biological chip prepared with the method, reactions of the chip cannot be inhibited, and the chip reacts very stably. With the adoption of the biological printing method, the preparation process of the chip is simplified, reaction results of the chip can be judged more quickly and more conveniently, besides, the detection throughput of the chip is increased, and accordingly, probes can be more accurately positioned on the chip.

Description

Technical field [0001] The invention belongs to the technical field of biochip preparation and application, and relates to a printing method for a biochip and its application. Background technique [0002] Biochip technology is a high-throughput molecular biology detection technology for multi-index detection. Biochips include protein chips and gene chips. The matrix of the chip includes glass, porcelain, nylon membrane and nitrocellulose membrane. Among them, glass-based chips generally use fluorescent labels to obtain chip detection signals; and membrane-based chips generally use enzymatic color development to determine the color points at different positions on the chip by the naked eye or a machine. In order to obtain the chip test results. [0003] The chip is also called a microarray, and each probe constituting the array detects different indicators. In order to achieve the purpose of multi-index detection, the entire chip needs to be divided into regions, so that when t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C40B50/06C40B40/06C40B40/10
CPCB01J19/0046B01J2219/00608B01J2219/00637B01J2219/00722B01J2219/00725C40B40/06C40B40/10C40B50/06
Inventor 杨华卫曾冀
Owner 杨华卫
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com