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Application of sophora tonkinensis endophytic bacterium B21 in preventing and controlling panax notoginseng black spot

An endophytic bacteria, B21 technology, applied in the biological field, can solve the problems of pollution, threat to health, economic loss of farmers, etc., and achieve broad application prospects and strong inhibitory effects.

Active Publication Date: 2016-04-06
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the traditional authentic production area of ​​Sanqi in Guangxi, due to the low altitude and high temperature and humidity weather conditions, this disease often occurs at the same time, resulting in a large amount of production reduction or even failure of Sanqi, and brought huge economic losses to the growers.
In order to control this fungal disease, a large amount of chemical pesticides are used, resulting in a large amount of pesticide residues in Panax notoginseng products, seriously polluting the environment, and greatly threatening people's health

Method used

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  • Application of sophora tonkinensis endophytic bacterium B21 in preventing and controlling panax notoginseng black spot
  • Application of sophora tonkinensis endophytic bacterium B21 in preventing and controlling panax notoginseng black spot
  • Application of sophora tonkinensis endophytic bacterium B21 in preventing and controlling panax notoginseng black spot

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Experimental program
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Effect test

Embodiment 1

[0022] Isolation, screening and identification of strains

[0023] 1. Isolation of strains

[0024] Test material: wild Vietnamese pagoda tree collected from the limestone area of ​​Tiandeng County, Guangxi.

[0025] Tested strains: provided by Institute of Plant Pathology, Guangxi University.

[0026] Medium: 1000ml NA medium: 3g of beef extract, 1g of yeast extract, 5g of peptone, 10g of sucrose, 15g of agar, pH7.0.

[0027] Surface disinfection: Rinse the fresh and healthy Vietnamese Sophorae root with a length of 6-8cm and a width of 1-2cm with running water for 30 minutes to wash away the sediment, then rinse the washed Vietnamese Sophorae root with sterile water twice, and air-dry the surface moisture , moved to the ultra-clean workbench, and under aseptic conditions, the Sophora japonica root was soaked in 75% ethanol for 1 min, rinsed with sterile water twice, soaked in sodium hypochlorite (available chlorine 1%) for 2 min, washed with sterile water for 3 times , dr...

Embodiment 2

[0080] Inhibitory Effects of Strain Metabolites on Panax notoginseng Black Spot

[0081] 1. Fermentation of strains and extraction of B21 metabolites

[0082] Inoculate the endophytic bacteria B21 of Sophora vietnamese into a 2-liter Erlenmeyer flask containing 1000ml of NB liquid medium (3g of beef extract, 1g of yeast extract, 5g of peptone, 10g of sucrose, pH7. 130r / min shaker fermentation culture for 10 days, the obtained fermented product was concentrated and dried under reduced pressure, added 2 times the methanol of the fermented product and ultrasonically ultrasonicated for 40 minutes, ultrasonically centrifuged, and the supernatant obtained after centrifugation was collected and concentrated under reduced pressure to obtain the strain The methanol crude extract of the fermentation product is the metabolite of endophytic bacteria B21 of Sophora vietnamese.

[0083] 2. Inhibitory effect of metabolites of endophytic bacteria B21 from Sophora japonica on the mycelial gro...

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Abstract

The invention discloses a sophora tonkinensis endophytic bacterium B21. The sophora tonkinensis endophytic bacterium B21 is classified and named as Burkholderia sp. B21, wherein the 16S rDNA gene sequence table of the strain is as described by SEQ ID NO.1, the depository authority is the General Microbiological Collection Center of China Committee for Culture Collection of Microorganisms, the depository address is Institute of Microbiology, Building 3, No.1, Beichen West Road, Chaoyang District, Beijing, the depository date is 13 May 2015, and the depository number is CGMCC No.10806. According to the sophora tonkinensis endophytic bacterium B21 provided by the invention, a strain of endophytic bacterium Burkholderia sp. B21 is separated and screened from the root of a medicinal plant sophora tonkinensis for the first time, the Burkholderia sp. B21 has a strong inhibiting effect on panax notoginseng alternaria panax whetzel, and a wide application prospect is created for the biological prevention and control field of panax notoginseng fungal diseases.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of a kind of endophytic bacterium B21 of Sophora japonica in the prevention and treatment of Panax notoginseng black spot. Background technique [0002] The prevention and control of plant diseases in modern agriculture is overly dependent on the use of chemical pesticides. The application of a large number of chemical pesticides not only has long-term damage to the ecological environment, but also causes the quality of agricultural products to decline, pesticide residues exceed the standard, pathogenic bacteria are resistant to drugs, and are harmful to humans and animals. Many questions. It is of great significance to find safer and more effective methods of disease control, and the use of biological methods to prevent and control plant diseases can effectively solve the above problems. [0003] Panax notoginseng F.H.chen, also known as Tianqi, Jinbuhuan, etc., has...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04A01N63/02A01P3/00C12R1/01
CPCA01N63/10C12P1/04C12N1/205C12R2001/01
Inventor 李良波姚裕群鲁璇黄荣韶
Owner GUANGXI UNIV
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