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Short and dense trichoderma brevicompactum and application thereof in cucumber fusarium wilt prevention and control

A technology of Trichoderma brevis and strains, applied in the field of Trichoderma brevis and its application in the control of cucumber wilt

Inactive Publication Date: 2016-04-06
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The control effect of the currently researched biocontrol agents on cucumber wilt needs to be improved, and it is necessary to find biocontrol agents with better control effects

Method used

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  • Short and dense trichoderma brevicompactum and application thereof in cucumber fusarium wilt prevention and control
  • Short and dense trichoderma brevicompactum and application thereof in cucumber fusarium wilt prevention and control
  • Short and dense trichoderma brevicompactum and application thereof in cucumber fusarium wilt prevention and control

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Experimental program
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Effect test

Embodiment 1

[0042] Embodiment 1, the separation and identification of Trichoderma brevis BF06

[0043] 1.1 Strain isolation

[0044] Trichoderma brevis BF06 was isolated from the forest soil of Fenghuangling, Haidian District, Beijing. The specific separation and screening method is as follows: take 10g soil sample and add it to a conical flask containing 100ml sterile water and glass beads, shake it on a shaker at 100 rpm for 20min, take 0.5ml and add it to 4.5ml sterile water, and then dilute it for 10 -2 , 10 -3 , 10 -4Take 0.1ml of the above soil suspension and spread it evenly on the PDA medium plate, and blow it in the ultra-clean workbench until it is slightly dry; each concentration is repeated 3 times, and cultured in a 25-28°C incubator. Take a single colony for dilution and streak isolation to obtain pure culture strains. Taking Fusarium oxysporum Cucumber specialization as the target bacteria, the antibacterial activity of pure culture strains was detected by plate confron...

Embodiment 2

[0053] Embodiment 2, Trichoderma brevis BF06 control cucumber wilt

[0054] 1. Trichoderma brevis BF06 inhibits Fusarium oxysporum cucumber-specific type

[0055] Trichoderma brevis BF06 and Cucumber specialization of Fusarium oxysporum (Shen Wenyuan, Biological characteristics of four specializations of Fusarium cucurbita and biological control of Fusarium cucurbita, master's thesis, 2012) were transferred to PDA culture After activation on the base, after culturing at 26°C for 48 hours, a 0.6mm-diameter hole punch was used to take the bacterial cake at the edge of the colony, and inoculated the bacterial cake of Trichoderma brevis BF06 to one end of the petri dish (3cm from the edge of the petri dish), and Fusarium oxysporum Cucumber specialized fungus cake was inoculated into the other end of the petri dish (3cm away from the edge of the petri dish), and the experiment was repeated 3 times. At the same time, only the treatment of Cucumber specialization of Fusarium oxysporu...

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Abstract

The invention discloses short and dense trichoderma brevicompactum and application thereof in cucumber fusarium wilt prevention and control. According to the short and dense trichoderma brevicompactum, the strain number is BF06, and the registering and booking serial number of the short and dense trichoderma brevicompactum in the China General Microbiological Culture Collection Center is CGMCC No.11845. The short and dense trichoderma brevicompactum BF06 has the significant antagonistic action on fusarium oxysporum f.sp cucumerinum which causes the cucumber fusarium wilt; conidial suspension of the strain and ordinary cultivation soil are mixed to serve as a biocontrol matrix, therefore, the cucumber fusarium wilt can be effectively prevented and controlled, and the prevention and control effect on the cucumber fusarium wilt is 90.4%. Accordingly, an excellent strain resource is supplied to pollution-free production of cucumbers in a protected area, and the practical value on prevention and control over the cucumber fusarium wilt, especially cucumber seedling stage fusarium wilt, is achieved.

Description

technical field [0001] The invention belongs to the technical field of microbial pesticides, and in particular relates to a strain of Trichoderma brevis and its application in the prevention and treatment of cucumber wilt. Background technique [0002] Cucumber Fusarium wilt, also known as "wilting disease", "vine cutting disease" and "dead seedling disease", is a systemic disease caused by Fusarium oxysporum f.sp.cucumerinum. , soil, fertilizers, irrigation water, insects, farm tools, etc., and is infected by soil; from the root or root neck, invades through the root wound and the intercellular space at the top of the root hair; parasitizes and reproduces in the vascular bundle, and expands upwards, blocking the duct and producing Toxins cause cell death (ductal wilt), which is one of the most difficult diseases in cucumber production and often causes large losses. [0003] The pathogenic bacteria overwinter on the soil, diseased residues and seeds with mycelium, sclerotia...

Claims

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Application Information

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IPC IPC(8): C12N1/14A01N63/04A01P3/00C12R1/885
CPCA01N63/30C12N1/14C12N1/145C12R2001/885
Inventor 李进一卢彩鸽刘霆吴慧玲张殿朋刘德文刘伟成
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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