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Detection method for fatty acid and vitamin E in idesia

A detection method, fatty acid technology, which is applied in the field of detection of characteristic components in Jatropha, can solve the problems of inaccurate and cumbersome detection results, and achieve the effects of good stability, high precision, and easy operation

Active Publication Date: 2016-03-30
CHENGDU PUSH BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The present invention aims to solve the problem in the prior art of using GC or GC / MS method to detect the presence of fatty acids in Jatropha: complex derivatization before detection, incomplete derivatization leads to inaccurate detection results, and provides an applicable The detection method of fatty acids and vitamin E in jatropha is carried out by HPLC-ELSD method. By strictly controlling the extraction of the sample solution, the HPLC separation process and the ELSD detection process, vitamin E and fatty acids in jatropha are detected at the same time. Pre-column derivatization avoids cumbersome pretreatment, the obtained results have good stability, high precision, and easy operation, and realize the evaluation of the quality of Jatropha by HPLC-ELSD method

Method used

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  • Detection method for fatty acid and vitamin E in idesia
  • Detection method for fatty acid and vitamin E in idesia
  • Detection method for fatty acid and vitamin E in idesia

Examples

Experimental program
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Embodiment 1

[0037] A kind of detection method of fatty acid and vitamin E in jatropha, described detection method is HPLC-ELSD method, described method comprises the steps:

[0038] (1) Preparation of sample solution: take 10 g of Jacarina extract, add 30 mL of methanol to dissolve it ultrasonically, cool to 20°C and centrifuge, take the supernatant as the sample solution; in this example, the Jacarica extract was extracted by Soxhlet extraction method, The method is as follows: take an appropriate amount of jatropha powder, wrap it with filter paper, put it in a Soxhlet extractor, add 10 times the volume of n-hexane, reflux at 70°C until the leachate is colorless, and concentrate the extract to dryness to obtain the jacarnia extract. thing.

[0039] (2) Simultaneously detect the content of fatty acids and vitamin E in the sample solution in step (1) by HPLC-ELSD method, detection instrument: Agilent (1260)

[0040] The condition of HPLC in the described HPLC-ELSD method is:

[0041] Ch...

Embodiment 2

[0067] A kind of detection method of fatty acid and vitamin E in jatropha, described detection method is HPLC-ELSD method, described method comprises the steps:

[0068] (1) Preparation of the sample solution: Take 5 g of Jacarina extract, add 75 mL of methanol to dissolve it ultrasonically, cool to 10°C and centrifuge, take the supernatant as the sample solution; in this example, the Jacarica extract was extracted by Soxhlet extraction method, The method is as follows: take an appropriate amount of jatropha powder, wrap it with filter paper, place it in a Soxhlet extractor, add 3 times the amount of n-hexane, reflux at 70°C until the leachate is colorless, and concentrate the extract to dryness to obtain the jacarnia extract .

[0069] (2) Simultaneously detect the content of fatty acids and vitamin E in the sample solution in step (1) by HPLC-ELSD method, detection instrument: AgilentHPLC

[0070] The condition of HPLC in the described HPLC-ELSD method is:

[0071] The col...

Embodiment 3

[0086] The difference between this embodiment and Example 2 is that, in this embodiment, the Jacarina extract is obtained by ultrasonic extraction, and the ultrasonic extraction method is as follows: take an appropriate amount of Jacarina powder, add 5 times of methanol, ultrasonic 30min, place Continue soaking in the dark for 4h, repeat twice, combine the extracts, concentrate until dry to obtain the Jatropha japonica extract. Detected by the method of this embodiment, the spectrum is obtained as figure 1 8, and the retention time and peak area statistics are in the following table 9, and the calculated content statistics are in the following table 9;

[0087] Table 9:

[0088]

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Abstract

The invention provides a detection method for fatty acid and vitamin E in idesia. The detection method is an HPLC-ELSD (High Performance Liquid Chromatography-Evaporative Light Scattering Detector) method and comprises the following steps: (1) preparing a sample solution; (2) synchronously detecting the contents of fatty acid and vitamin E in the sample solution of the step (1) with the HPLC-ELSD method, wherein the conditions of an HPLC are as follows: a chromatographic column is C18, a mobile phase is the mixture of methanol and diluted acid water or the mixture of acetonitrile and the diluted acid water, and the column temperature of the chromatographic column is 20 DEG C-50 DEG C and the like, and detection conditions of an ELSD are as follows: the temperature of a drift tube is 50 DEG C-80 DEG C, an air flow is 2.0L / min-3.5L / min, a flow distribution manner is adopted and the flow distribution ratio ranges from (1:4) to (2:3). According to the method provided by the invention, the HPLC-ELSD method is used for determining the contents of fatty acid and vitamin E in the idesia for the first time; and an obtained result has good stability and high precision and the operation is simple and convenient.

Description

technical field [0001] The invention relates to the technical field of detection of characteristic components in jacarnia, in particular to a method for detecting fatty acids and vitamin E in jacaranda. Background technique [0002] Idesia polycarpa Maxim is a deciduous tree of the genus Idesia polycarpa Maxim. There is only one species of this genus in the world. It is mainly produced in Japan, Korea, the Russian Far East and China. In my country, it is distributed in the provinces south of the Qinling Mountains and the Huaihe River. Jatropha belongs to woody oil tree species, and its fruit has high oil content. Jatropha oil is a semi-natural oil, which is an excellent edible oil after refining, and is rich in oleic acid, linoleic acid, linolenic acid, palmitic acid, etc. Saturated oil ingredients, edible unsaturated fatty acids have the effects of lowering blood lipids, preventing atherosclerosis, anti-arrhythmia, and ensuring fetal brain development. Edible vitamin E is ...

Claims

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Application Information

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IPC IPC(8): G01N30/88G01N30/06G01N30/02
CPCG01N30/02G01N30/06G01N30/88G01N2030/027
Inventor 肖斌陈冲朱甜甜夏柯刘丁
Owner CHENGDU PUSH BIOLOGICAL TECH
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