Accurate, quick and noninvasive method for instilling medicine into rat

A fast, infusion technology, applied in the field of clinical medicine and animal experiments, can solve the problems of increasing the pain and discomfort of mice, the long time spent on each animal, and the influence of repeated intubation, so as to achieve easy access, simple production, and increased The effect of consumption

Inactive Publication Date: 2016-03-30
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, the invasive intubation method will cause damage to the surrounding tissues of the trachea and throat, thereby affecting the repeated intubation, thereby affecting the results of the entire experiment; Although it is a non-invasive tracheal instillation, the infusion operation takes a long time for each animal; the infusion volume is not accurate, and the intubation operation will also increase the pain and discomfort of the mice

Method used

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  • Accurate, quick and noninvasive method for instilling medicine into rat
  • Accurate, quick and noninvasive method for instilling medicine into rat
  • Accurate, quick and noninvasive method for instilling medicine into rat

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Embodiment 1 Accurate and rapid non-invasive instillation method for rats

[0031] 1. Materials and methods

[0032]1.1 Materials

[0033] Iron stand, pipette gun, gun tip, pediatric laryngoscope, string, anesthesia tank, anhydrous ether, cotton, timer.

[0034] 25 male healthy SD rats, body weight 230-260g, (SPF grade, provided by Dongchuang Experimental Animal Science and Technology Service Department, Kaifu District, Changsha City, license number: SCXK (Xiang) 2009-0012). The experimental animals were fed in separate cages, the indoor temperature was kept at 22±2°C, and the ventilation was maintained. The bedding was changed every 2 days, and the cages were cleaned every 2 weeks for disinfection with Bath disinfectant every week.

[0035] 1.2 Operation method of instillation in rats

[0036] 1.2.1 Production of anesthesia tank: In this experiment, a transparent plastic tank was selected as the anesthesia tank. Put cotton into the tank to cover the bottom of the a...

Embodiment 2

[0046] Embodiment 2 Accurate and rapid non-invasive instillation method for rats

[0047] 1. Materials and methods

[0048] 1.1 Materials

[0049] Iron stand, pipette gun, gun tip, pediatric laryngoscope, string, anesthesia tank, anhydrous ether, cotton, timer

[0050] 25 male healthy SD rats, body weight 245-290g, (SPF grade, provided by Dongchuang Experimental Animal Science and Technology Service Department, Kaifu District, Changsha City, license number: SCXK (Xiang) 2009-0012). The experimental animals were fed in separate cages, the indoor temperature was kept at 22±2°C, and the ventilation was maintained. The bedding was changed every 2 days, and the cages were cleaned every 2 weeks for disinfection with Bath disinfectant every week.

[0051] 1.2 Operation method of instillation in rats

[0052] 1.2.1 Production of anesthesia tank: In this experiment, a transparent plastic tank was selected as the anesthesia tank. Put cotton into the tank to cover the bottom of the a...

Embodiment 3

[0062] Example 3 Accurate and fast non-invasive instillation method for rats

[0063] 1. Materials and methods

[0064] 1.1 Materials

[0065] Iron stand, pipette gun, gun tip, pediatric laryngoscope, string, anesthesia tank, anhydrous ether, cotton, timer

[0066] 25 male healthy SD rats, body weight 265-326g, (SPF grade, provided by Dongchuang Experimental Animal Science and Technology Service Department, Kaifu District, Changsha City, license number: SCXK (Xiang) 2009-0012). The experimental animals were fed in separate cages, the indoor temperature was kept at 22±2°C, and the ventilation was maintained. The bedding was changed every 2 days, and the cages were cleaned every 2 weeks for disinfection with Bath disinfectant every week.

[0067] 1.2 Operation method of instillation in rats

[0068] 1.2.1 Production of anesthesia tank: In this experiment, a transparent plastic tank was selected as the anesthesia tank. Put cotton into the tank to cover the bottom of the anest...

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Abstract

The invention provides an accurate, quick and noninvasive method for instilling a medicine into a rat. According to the method, the rat is narcotized by aether and hung on an iron stand, a tongue of the rat is clamped and pulled out of a mouth by tweezers coating with gauze, a glottis is found under the exposure of a laryngoscope, a nose of the rate is squeezed to enable the rat to breathe by the mouth, a pipette with the sucked instillation medicine is inserted into the glottis quickly with the opening and closing of the glottis, and the medicine is instilled into a trachea. The pipette is taken out, a user squeeze the nose continuously for 3-8 seconds to enable the instillation medicine to be inhaled automatically through the breath of the rat, and the instillation is successful when the user hears moist rale. The accurate, quick and noninvasive method for instilling the rat has the advantages of being noninvasive, free of pain, economical, quick and accurate and has remarkable advantages compared with other instillation methods.

Description

technical field [0001] The invention relates to the fields of clinical medicine and animal experiments, in particular to a precise, rapid and non-invasive instillation method for rats. Background technique [0002] Respiratory exposure is one of the most important exposure routes in clinical medicine, toxicology, environmental medicine and other fields of research. With the rapid development of research in various disciplines in recent years, the demand for animal experiments of drug administration through the respiratory tract, drug exposure, and respiratory monitoring is on the rise. The trend is that rats are internationally standardized experimental animals and are widely used in various medical and biological experiments. In animal experiments such as ventilator-assisted breathing, respiratory function testing or intratracheal drug administration in rat thoracotomy , smooth rat tracheal intubation is the prerequisite for the success of the experiment. At present, the co...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61D7/00A61D7/04
Inventor 刘仲华刘建军林勇林海燕洪乐乐
Owner HUNAN AGRICULTURAL UNIV
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