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Water-soluble protein-phytosterin nanometer particles as well as preparation and application

A technology for water-soluble protein and phytosterol, which is applied in the fields of water-soluble protein-phytosterol nanoparticles and their preparation and application, can solve the problems of poor solubility of phytosterol and the like, and achieves improved water solubility and loading rate, high embedding rate, and water solubility good effect

Active Publication Date: 2016-03-23
SOUTH CHINA UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the shortcomings and deficiencies of the above prior art, the primary purpose of the present invention is to provide a method for preparing water-soluble protein-phytosterol nanoparticles, aiming to utilize the nano-embedding properties of proteins and the interaction between proteins and phytosterols function, quickly and effectively form nanoparticles, and then solve the technical bottleneck problem of poor solubility of phytosterols in the water phase

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Under the conditions of normal temperature and mechanical stirring or magnetic stirring, the soybean protein isolate is dispersed and dissolved in deionized water according to the ratio of solid-liquid ratio (kg: liter) of 0.1:10, and then placed in a refrigerator at 4°C to fully water for 6 hours, and finally centrifuged to remove insoluble matter to obtain a protein solution;

[0032] (2) According to the solid-to-liquid ratio (g:l) of 5:1, add phytosterols into n-hexane, heat at 45°C and fully dissolve under magnetic stirring conditions for 4 minutes to obtain a phytosterol solution;

[0033] (3) The phytosterol solution is added to the protein solution at a volume ratio of 10:100, homogenized for 5 minutes at a speed of 5000 rpm, and then the homogenized emulsion is micro-jetted at a pressure of 40 MPa. The number of times is 1 time to obtain a mixed emulsion;

[0034] (4) Collect the mixed emulsion obtained in step (3) in a distillation flask, remove n-hexane ...

Embodiment 2

[0037] (1) Disperse and dissolve whey protein concentrate in deionized water at a solid-to-liquid ratio (kg:l) of 0.1:10 under normal temperature and mechanical stirring or magnetic stirring conditions, and then place it in a refrigerator at 4°C for Hydrate for 10 hours, and finally centrifuge to remove insoluble matter to obtain a protein solution;

[0038] (2) Add phytosterols into n-hexane according to the solid-to-liquid ratio (gram:liter) of 5:1, and fully dissolve them for 5 minutes under heating at 42°C and magnetic stirring to obtain a phytosterol solution;

[0039] (3) The phytosterol solution is added to the protein solution at a volume ratio of 10:100, homogenized for 4 minutes at a speed of 6000 rpm, and then the homogenized emulsion is micro-jetted at a pressure of 50 MPa. The number of times is 1 time to obtain a mixed emulsion;

[0040] (4) Collect the mixed emulsion obtained in step (3) in a distillation flask, remove n-hexane by rotary evaporation at 45°C and...

Embodiment 3

[0043] (1) Disperse and dissolve sodium caseinate in deionized water according to the solid-liquid ratio (kg:l) of 0.3:10 under normal temperature and mechanical stirring or magnetic stirring conditions, and then place it in a refrigerator at 4°C for Hydrate for 8 hours, and finally centrifuge to remove insoluble matter to obtain a protein solution;

[0044] (2) Add phytosterols into n-hexane according to the solid-to-liquid ratio (g:l) of 5:1, and fully dissolve them for 5 minutes under heating at 45°C and magnetic stirring to obtain a phytosterol solution;

[0045] (3) The phytosterol solution is added to the protein solution at a volume ratio of 10:100, homogenized for 5 minutes at a speed of 5000 rpm, and then the homogenized emulsion is micro-jetted at a pressure of 40 MPa. The number of times is 1 time to obtain a mixed emulsion;

[0046] (4) Collect the mixed emulsion obtained in step (3) in a distillation flask, remove n-hexane by rotary evaporation at 45°C and a vacu...

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Abstract

The invention belongs to the technical field of processing of functional foods, and discloses water-soluble protein-phytosterin nanometer particles as well as preparation and application. The preparation method comprises the following steps of dispersing and dissolving sodium caseinate, soy protein isolate or whey protein concentrate in deionized water so as to obtain a solution; then placing the solution at the temperature of 0-10 DEG C for hydration for 6-12 hours so as to obtain a protein solution; dispersing and dissolving phytosterin in N-hexane so as to obtain a phytosterin solution; then adding the phytosterin solution to the protein solution, and performing homogenizing treatment and micro jet treatment so as to obtain mixed emulsion; and finally performing steaming so as to remove the N-hexane in the mixed emulsion, and performing freeze drying so as to obtain the water-soluble protein-phytosterin nanometer particles. The preparation method disclosed by the invention is simple, low in cost, safe and environment-friendly; the obtained water-soluble protein-phytosterin nanometer particles are small in particle diameters, good in water solubility and high in embedding rate, and can be used as functional factors to be used for the production of functional foods or medicines.

Description

technical field [0001] The invention belongs to the technical field of functional food processing, and specifically relates to a water-soluble protein-phytosterol nanoparticle and its preparation and application. Background technique [0002] Phytosterols are analogues of sterols and are listed as functional oils. They are slightly white powder at room temperature, odorless and tasteless, and their melting point can reach 130-140°C. Phytosterols in nature have dissociation and esterification properties. Phytosterols are plant compounds that have a similar chemical structure and biological function to cholesterol. Studies have shown that phytosterols play an important role in inhibiting tumors, preventing heart disease, anti-cancer, preventing and treating prostate diseases, and regulating immune function. The most eye-catching It is a cholesterol-lowering effect. A daily dose of 2–3 grams of phytosterols has been shown to lower LDL cholesterol by 5–15%. In September 2000, ...

Claims

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Application Information

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IPC IPC(8): A23L33/10A23L5/00
Inventor 唐传核曹文君
Owner SOUTH CHINA UNIV OF TECH
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