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A kind of method that biological enzyme catalyzes preparation phospholipid type DHA

A phospholipid-type, biological enzyme technology, applied in the direction of fermentation, etc., can solve the problems of low conversion rate of transesterification reaction, complex components of soybean phospholipids, unfavorable industrial production, etc., to avoid separation and purification operations, improve reaction efficiency and conversion rate, The effect of reducing equipment investment

Active Publication Date: 2019-09-20
XIAMEN KINGDOMWAY BIOTECH CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0014] Above-mentioned this class synthetic method, not only there is soybean phospholipid composition complexity (mostly is the mixture that phosphatidylcholine, phosphatidylethanolamine, phosphatidylinositol, phosphatidylserine and phosphatidic acid form), the defect that selectivity is not high; Algae oil or fish oil DHA needs to be dissociated into free docosahexaenoic acid before transesterification. The steps are cumbersome and the conversion rate of transesterification is not high, which is not conducive to industrial production.

Method used

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  • A kind of method that biological enzyme catalyzes preparation phospholipid type DHA
  • A kind of method that biological enzyme catalyzes preparation phospholipid type DHA
  • A kind of method that biological enzyme catalyzes preparation phospholipid type DHA

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] (1) Weigh 5.0g of algal oil DHA glyceride and dissolve it in 25mL of n-hexane to obtain the n-hexane solution of algal oil DHA ester; add 0.31g of Novozyme435 immobilized lipase and 0.30g of water, then add 0.025g of polyglycerol fatty acid ester, adjust the pH value of the mixture to be 6.7; 2 Place it on a constant temperature water bath and a magnetic stirring device under the protection of the hood, heat it to about 50°C in advance, and stir at a speed of 125r / min for 15-30min while heating. Slowly add phosphatidylserine (purity: 25%) n-hexane solution (1.01g phosphatidylserine is dissolved in 7mL n-hexane) dropwise under stirring, N 2 Under protection, the reaction was stirred at a constant speed for 18 hours under the condition of a water bath at 50°C.

[0057] (2) After the reaction, stand and filter to recover the catalyst biological enzyme; add 30mL of water to the organic phase to wash, then distill under reduced pressure in a water bath at 60°C to recover th...

Embodiment 2

[0059] (1) Weigh 6.0g of algal oil DHA glyceride and dissolve it in 42mL of n-hexane to obtain the n-hexane solution of algal oil DHA ester; add 0.72g of phospholipase A1 and 0.06g of water, and then add 0.06g of polyoxyethylene Sorbitan monooleate, adjust the pH value of the mixture to be 7.2; the above mixed solution in an inert gas N 2 Place it on a constant-temperature water bath and a magnetic stirring device under the protection of the hood, heat to 50°C in advance, and stir at a speed of 130r / min for 15-30min while heating. While stirring, a n-hexane solution (0.97 g of phosphatidylserine dissolved in 5 mL of n-hexane) dissolved in phosphatidylserine (purity: 50%) was slowly added dropwise. N 2 Under protection, the reaction was stirred at a constant speed in a water bath at 60°C for 27 hours.

[0060] (2) After the reaction was completed, the catalyst bio-enzyme was recovered by static filtration; the organic phase was washed with 30 mL of water, and then distilled u...

Embodiment 3

[0062] (1) Weigh 7.0g of algal oil DHA glyceride and dissolve it in 56mL of n-hexane to obtain the n-hexane solution of algal oil DHA ester; add 1.05g of phospholipase A2 and 0.28g of water, then add 0.06g of sucrose fatty acid ester, adjust the pH value of the mixture to 7.5; the above mixed solution in an inert gas N 2 Place it on a constant temperature water bath and a magnetic stirring device under the protection of the hood, heat to 50°C in advance, and stir at a speed of 150r / min for 15-30min while heating. Slowly add phosphatidylserine (purity: 50%) n-hexane solution (1.07g phosphatidylserine is dissolved in 7mL n-hexane) dropwise under stirring; N 2 Under protection, the reaction was stirred at a constant speed in a water bath at 60°C for 26 hours.

[0063] (2) After the reaction was completed, the catalyst bio-enzyme was recovered by static filtration; the organic phase was washed with 30 mL of water, and then distilled under reduced pressure in a water bath at 60° C...

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Abstract

The invention provides a method for preparing phospholipids type DHA through catalysis of a biological enzyme. The method includes the step of preparing phospholipids type DHA from phospholipids and substances with DHA through catalysis of the biological enzyme. DHA grease does not need to be dissociated into dissociative docosahexaenoic acid (DHA) in advance, the process operation is simple and safe, reaction byproducts are few, lots of products can be rapidly obtained after one-time operation, the operation process is simplified, and the investment of devices is reduced.

Description

technical field [0001] The invention relates to the field of biological enzyme catalyzed synthesis, in particular to a method for preparing phospholipid DHA by biological enzyme catalysis. Background technique [0002] DHA (docosahexaenoic acid) is an essential fatty acid that is difficult for the human body to synthesize and must be provided by food. It can make the cardiovascular and cerebrovascular soft and elastic, and play a very good role in promoting the protection and function of the brain nerves, so it is known as the "brain protection expert". Studies have shown that DHA can promote the growth and development of brain cells, regulate the antioxidant capacity of brain cells and scavenge free radicals, delay brain aging and prevent Alzheimer's disease, etc. It is a health guard covering infants to middle-aged and elderly people. play an important role in both. DHA also helps eye health, enhances the fluidity of retinal cell membranes, accelerates the transmission a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64
Inventor 许素霞刘华英陈芳芳
Owner XIAMEN KINGDOMWAY BIOTECH CO LTD
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