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Method for preparing diglyceride through enzyme catalysis

A technology of catalyzed esterification of diacylglycerol, which is applied in the field of food science, can solve the problems of deacidification of by-products and difficult control of hydrolysis, and achieve the effects of avoiding excessive hydrolysis, reducing production costs, and low acid value

Active Publication Date: 2016-03-16
HUAZHONG UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the above defects or improvement needs of the prior art, the present invention provides an enzyme-catalyzed preparation method of diglyceride, the purpose of which is to prepare diglyceride with high yield under mild conditions through lipase-catalyzed hydrolysis and re-esterification , so as to solve the technical problems that the hydrolysis of Chengdu in the existing preparation method is difficult to control or the by-product needs further deacidification treatment

Method used

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  • Method for preparing diglyceride through enzyme catalysis
  • Method for preparing diglyceride through enzyme catalysis
  • Method for preparing diglyceride through enzyme catalysis

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preparation example Construction

[0023] The preparation method of edible diglyceride provided by the invention comprises the following steps:

[0024] (1) Partial hydrolysis of oil: the edible oil is partially hydrolyzed by enzymatic hydrolysis, and the hydrolyzate is obtained after dehydration, so that the content of diglyceride in the hydrolyzate is between 30.0wt% and 35.0wt%, and the free fatty acid content Between 26.0wt% and 30.0wt%; the enzyme used is a lipase with a specific activity between 1,000,000U / g and 1,100,000U / g, and the added amount is 0.3%-1.5% of the mass of the edible oil.

[0025] The enzyme-catalyzed hydrolysis reaction conditions are as follows:

[0026] The oil-water ratio is 1:1.5-2.9g / mL, the reaction temperature is 35.0-60.0°C, and the reaction time is 3.0-10.0h.

[0027] (2) Re-esterification: Add glycerol with 2.5 times to 4 times its mass to the hydrolyzate obtained in step (1), use enzyme-catalyzed esterification to carry out esterification reaction to synthesize diglyceride, ...

Embodiment 1

[0035] A preparation method of edible diglycerides, comprising the following steps:

[0036] (1) Partial hydrolysis of oil: the commercially available rapeseed oil is partially hydrolyzed by an enzyme-catalyzed hydrolysis method, and the hydrolyzate is obtained after dehydration. The specific steps are as follows:

[0037] Add LipozymeTLIM, whose specific activity is 1,050,000 U / g, the amount of enzyme added is 0.43% of the edible oil mass, and placed in a constant temperature shaker at a temperature of 55.8° C. for 4.2 hours.

[0038] The content of diglyceride in the hydrolyzate is 33.08%, and the content of free fatty acid is 28%.

[0039] The reaction product was detected by HPLC-ELSD, and the results were as follows: figure 1 Shown, each component and its content, triglyceride (TAG): 38.715%, free fatty acid (FFAs): 23.252%, diglyceride (DAG): 32.256%, monoglyceride (MAG): 2.770%.

[0040] (2) Re-esterification: Add glycerin with 2.85 times its mass to the hydrolyzate o...

Embodiment 2

[0046] A preparation method of edible diglycerides, comprising the following steps:

[0047] (1) Partial hydrolysis of oil: The commercially available soybean oil is partially hydrolyzed by an enzyme-catalyzed hydrolysis method, and the hydrolyzate is obtained after dehydration. The specific steps are as follows:

[0048] Add LipozymeTLIM, whose specific activity is 1,080,000 U / g, the amount of enzyme added is 0.3% of the edible oil mass, and placed in a constant temperature shaker at a temperature of 60° C. for 10 hours.

[0049] The content of diglyceride in the hydrolyzate is 35.0wt%, and the content of free fatty acid is 26.0wt%.

[0050] (2) Re-esterification: Add glycerin 4 times its mass to the hydrolyzate obtained in step (1), use enzyme-catalyzed esterification to carry out esterification reaction to synthesize diglyceride, and obtain the edible diglycerides. The enzyme used is lipase Novozyme435 with a specific activity of 114,562 U / g, and the added amount is 0.6% ...

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Abstract

The invention discloses a method for preparing diglyceride through enzyme catalysis. The method is characterized by including the following steps that firstly, grease parts are hydrolyzed, wherein edible oil is partially hydrolyzed through an enzyme catalysis hydrolysis method, hydrolysis products are obtained through dewatering, the content of diglyceride in the hydrolysis products ranges from 30.0wt% to 35.0wt%, and the content of free fatty acid ranges from 26.0wt% and 30.0wt%; secondly, esterification is performed, glycerin with the mass 2.5-4 times of that of the hydrolysis products is added in the hydrolysis products obtained in the first step, an esterification reaction is performed through an enzyme catalysis esterification method, diglyceride is synthesized, and the edible diglyceride is obtained after excessive glycerin is removed. According to the method, free fatty acid does not need to be independently prepared, and operation is easy; excessive hydraolysis can be avoided, and total controllability is high; besides, the method is high in diglyceride yield, the acid value is low, acid removing treatment does not need to be performed on finished products, preparation cost is substantially reduced, and the method is suitable for large-scale industrial production.

Description

technical field [0001] The invention belongs to the field of food science, and more specifically relates to a method for preparing diglycerides. Background technique [0002] Diacylglycerol (DAG for short), also known as diacylglycerol, diglyceride, diglyceride, is a kind of product synthesized from one molecule of glycerol and two molecules of free fatty acid esterification or triglyceride (Triacylglycerol, TAG) is a structural lipid in which a fatty acid is replaced by a hydroxyl group. Recent studies have shown that DAG-rich edible oil, as a new type of functional oil, has a variety of physiological activities and functions, can prevent and treat fatty liver, cardiovascular and cerebrovascular diseases, does not accumulate in the body after eating, and has the ability to prevent obesity. And other important functions, widely used. As early as 1998, DAG oil was approved by the Japanese Ministry of Health, Labor and Welfare (MHLW, Japan Ministry of Health, Labor and Welfa...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64
CPCC12P7/6454
Inventor 闫云君曹海徐莉
Owner HUAZHONG UNIV OF SCI & TECH
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