Flow cytometric detection kits, methods and cell fixatives for human respiratory pathogens
A detection kit and flow cytometry technology, applied in the field of pathogen diagnosis, can solve problems such as technologies and products that can detect respiratory pathogens, and save resources and time, save labor costs, and achieve objective and accurate results.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Embodiment 1
[0031] Example 1 cell fixative
[0032] The cell fixative solution described in this embodiment contains a PBS solution with a volume fraction of 0.5% formaldehyde and a volume fraction of 60% methanol. The preparation method is as follows: adding formaldehyde and methanol to PBS solution, so that the volume fraction of formaldehyde is 0.5%, and the volume fraction of methanol is 60%, and the pH value is adjusted to 7.3 to obtain the product.
Embodiment 2
[0033] Embodiment 2: the flow cytometric detection kit of human respiratory tract pathogen
[0034] This embodiment provides a flow cytometric detection kit for human respiratory pathogen infection. The kit includes the cell fixative described in Example 1, cell permeabilization agent and fluorescein (FITC-fluorescein isothiocyanate) labeled monoclonal antibody against Adv antigen (purchased from Guangzhou Ruida Biotechnology Co., Ltd. ), flow cytometry cell washing solution. In this embodiment, the cell permeation agent is Triton-X100 solution with a volume fraction of 1%. The cell washing solution is PBST (1×, pH7.4)
Embodiment 3
[0035] Embodiment 3: the flow cytometry detection method of human respiratory tract pathogen
[0036] This embodiment is a method for detecting a single pathogen (Adv) infection using the flow cytometry kit in Example 2, and proceeds as follows:
[0037] 1. Bronchoalveolar lavage fluid sample collection and cell fixation: collected by professional medical staff, after sampling, take 3ml into the sampling tube. The sampling tube was centrifuged at 500 g for 8 minutes, the supernatant was discarded, and the cell layer was not sucked away. Add 3ml of cell fixative and resuspend the cells, and tighten the tube cap. Store the sampling tube upright at -20°C.
[0038] 2. Preparation of single cell suspension: place the above-mentioned sampling tube containing the specimen on a shaker for 10-15 seconds, take out and discard the swab. Centrifuge the above-mentioned sampling tube at 500g for 8 minutes, discard the supernatant, and be careful not to suck away the cell layer. Then add...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com