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Spine date seed extract and preparation method and application thereof

A jujube seed extract and extract technology, applied in the direction of drug combination, pharmaceutical formula, plant raw materials, etc., can solve the problem of low extraction rate of active substances with low polarity, so as to reduce the amount of human consumption, reduce the amount of ointment, The effect of simple process

Inactive Publication Date: 2016-03-09
SHENYANG PHARMA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, most of the extraction methods of jujube seed are water extraction, but the extraction rate of active substances with low polarity is not high by water extraction.

Method used

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  • Spine date seed extract and preparation method and application thereof
  • Spine date seed extract and preparation method and application thereof
  • Spine date seed extract and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Embodiment 1: preparation method of Suanzaoren extract

[0013] Precisely weigh 50g of jujube seed, put it in a 100ml round bottom flask, add 10 times the amount of water, 30%, 40% and 50% ethanol to reflux, and extract twice (60-65°C), each time for 1h. After the extract was concentrated by a rotary evaporator (60-65° C.), brown samples were obtained with yields of 10.1%, 7.1%, 6.4% and 5.7%.

Embodiment 2

[0014] Example 2: Determination of Jujube Seed Saponin Content in Suanzaoren Extract

[0015] The jujube seed extract obtained in Example 1 was used to determine the content of jujube seed saponins in the extract.

[0016] The invention adopts the HPLC method to be applied to the content determination of Suanzaoren saponin in Suanzaoren Decoction. Adopt ThermoBDSHYPERSILC-18 (4.6mm × 250mm; 5μm) chromatographic column, use acetonitrile (A)-water (B) as mobile phase, gradient elution (Table 1); Sour jujube seed extract is dissolved in water, as the test sample solution, the external standard method was used to calculate the content of jujuboside in the sample solution. The content of jujuboside is 25.4mg / g.

[0017]

Embodiment 3

[0018] Embodiment 3: Pharmacological activity experiment of Suanzaoren extract

[0019] The jujube seed extract prepared in Example 1 was used to conduct a pharmacological activity experiment.

[0020] Effects on the Number of Mice Falling to Sleep Induced by Subthreshold Dose of Pentobarbital Sodium

[0021] 70 male mice were randomly divided into 7 groups, 10 in each group. Each administration group was intragastrically administered with different extraction solvents 30g / kg (raw medicinal material) of Suanzaoren extract, 6.4g / kg (granule) of Suanzaoren granules, and 1.25mg / kg of diazepam. The blank control group was given the same volume of distilled water. 1 time a day for 5 consecutive days. Fast for 8 hours before the last administration, and water for the first 1 hour. After 30 minutes of administration, intraperitoneally inject pentobarbital sodium 30 mg / kg, observe and record the number of sleeping mice. If the varus righting reflex disappears for more than 1min, i...

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Abstract

The invention belongs to the field of traditional Chinese medicine pharmaceutical manufacturing, and particularly relates to a preparation method of single-medicine spine date seed extract and application of the spine date seed extract in calming and hypnosis drugs. The preparation method is simple in technology, low in cost and suitable for industrial preparation. The method for preparing the spine date seed extract comprises the following steps that 1, the medicinal material of spine date seeds is weighed; 2, reflux extraction is conducted twice through ethyl alcohol with the weight being 10 times that of the medicinal material and the volume concentration of 30%-50%, and reflux extraction is conducted for 0.5-2 h each time; 3, an extracted solution is concentrated to obtain dry extract or is freeze-dried to obtain the spine date seed extract. The invention further provides the application of the spine date seed extract in preparation of the calming and hypnosis drugs. It is indicated through pharmacology experiments that the hypnotic effect is significant when extraction is conducted through the ethyl alcohol of 30% and has no obvious difference compared with that of water extract.

Description

technical field [0001] The invention belongs to the field of traditional Chinese medicine pharmacy, and specifically relates to a preparation method of a single herbal jujube seed extract and its application in sedative and hypnotic drugs. The process is simple, low in cost and suitable for industrial preparation. Background technique [0002] Semen Ziziphi Spinosae (Semen Ziziphi Spinosae), a traditional Chinese medicine, is the dried and mature seed kernel of Ziziphous jujube Mill.var.spinosa (Bunge) Hu.et H.f.Chou, a plant of Rhamnaceae. , Concentrate sweat, produce body fluid, and nourish the liver. It is used for insomnia, palpitations and dreaminess, body deficiency and hyperhidrosis, and thirsty wounds. It is a traditional sedative and calming medicine. Clinically, it is mostly used to treat insomnia and anxiety disorders. Suanzaoren, as the first batch of dual-use medicine and food promulgated by the Ministry of Health, bears the brunt of the research hotspot. Suan...

Claims

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Application Information

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IPC IPC(8): A61K36/725A61P25/20
CPCA61K36/725A61K2236/333A61K2236/39A61K2236/51
Inventor 袁子越李清毕开顺杜易洋钟炎
Owner SHENYANG PHARMA UNIVERSITY
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