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Chicken infectious bronchitis virus diluent and preparation method thereof

A technology for bronchitis and chicken infectivity, which is applied in chicken infectious bronchitis virus dilution and its preparation field, can solve the problems of lowering the poison price and low content of allantoic fluid, and achieve the increase of allantoic fluid volume and the reduction of allantoic fluid The effect of increasing the volume and improving the ability to withstand viruses

Inactive Publication Date: 2016-03-02
浙江美保龙生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Usually, 10-day-old chicken embryos are used for Newcastle disease virus inoculation and breeding. Generally, chicken embryo fluid is harvested after 96-120 hours of cultivation, that is, 14-15 days old. At this time, the age is too old and the allantoic fluid content is not high (chicken embryo The allantoic fluid content is the highest at the age of 13 to 14 days of development); if the allantoic fluid content is increased, it must be harvested in advance, but the virus content has not reached the peak at this time, and the reduction of the poison price is not worth the loss.

Method used

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  • Chicken infectious bronchitis virus diluent and preparation method thereof
  • Chicken infectious bronchitis virus diluent and preparation method thereof
  • Chicken infectious bronchitis virus diluent and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] A chicken infectious bronchitis virus dilution, the virus dilution contains per 1000ml of phosphate buffer: 3g of amino acid combination (including 0.75g of methionine, 0.9g of tryptophan, 0.75g of tyrosine, and 0.6g of cystine) , vitamin combination 4g (vitamin B21.2g, vitamin D1.6g and vitamin E1.2g), compound Chinese medicine polysaccharide 20g, sodium selenite 16mg, the pH of the diluent is 7.0.

[0036] A kind of preparation method of chicken infectious bronchitis virus dilution, its steps are:

[0037] (1) Preparation of phosphate buffer solution: Accurately weigh 8 g of sodium chloride, 0.2 g of potassium chloride, 1.15 g of disodium hydrogen phosphate, 0.2 g of potassium dihydrogen phosphate, and 0.1 g of calcium chloride according to the above formula of phosphate buffer solution. Dissolve 0.1 g of magnesium chloride containing 6 crystal waters in 1000 ml of double distilled water, pass through a 0.22 μm filter membrane under aseptic conditions, and filter and ...

Embodiment 2

[0047] A chicken infectious bronchitis virus dilution, the virus dilution per 1000ml phosphate buffer (the phosphate buffer is composed of sodium chloride 6g, potassium chloride 0.05g, disodium hydrogen phosphate 1g, potassium dihydrogen phosphate 0.05g, Calcium chloride 0.05g, magnesium chloride 0.05g containing 6 crystal waters dissolved in 1000ml double distilled water) contains: amino acid combination 4g (including methionine 0.8g, tryptophan 0.8g, tyrosine 1.2g, cystine 1.2g), vitamin combination 5g (including vitamin B22g, vitamin D1g and vitamin E2g), compound Chinese medicine polysaccharide 25g (extracted from 10 parts of curculigo, 10 parts of Eucommia ulmoides, 20 parts of poria cocos and 5 parts of intermittent), selenous acid Sodium 12 mg, the pH of this dilution is 7.0.

[0048] A kind of preparation method of chicken infectious bronchitis virus diluent, its steps are the same as embodiment 1.

Embodiment 3

[0050] A chicken infectious bronchitis virus dilution, the virus dilution per 1000ml phosphate buffer (the phosphate buffer is composed of sodium chloride 10g, potassium chloride 0.5g, disodium hydrogen phosphate 1.2g, potassium dihydrogen phosphate 0.5g , calcium chloride 0.2g, magnesium chloride 0.2g containing 6 crystal waters dissolved in 1000ml double distilled water to obtain) contains: amino acid combination 1g (wherein methionine 0.3g, tryptophan 0.4g, tyrosine 0.1g, cystine acid 0.2g), vitamin combination 1g (including vitamin B20.15g, vitamin D0.5g and vitamin E0.35g), compound Chinese medicine polysaccharide 5g (from 30 parts of curculigo, 20 parts of Eucommia, 40 parts of poria cocos and 20 parts of intermittent extracted), sodium selenite 18mg, and the pH of the diluent is 6.6.

[0051] A kind of preparation method of chicken infectious bronchitis virus diluent, its steps are the same as embodiment 1.

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Abstract

The invention relates to a chicken infectious bronchitis virus diluent and a preparation method thereof and belongs to the technical field of animal biological products. Every 1000 ml of phosphate buffer in the diluent comprises, by weight, 1-4 g of amino acid combination, 1-5 g of vitamin combination, 12-18 mg of sodium selenite and 5-25 g of compound traditional Chinese medicine polysaccharide. The amino acid combination comprises methionine, tryptophan, tyrosine and leucine. The vitamin combination comprises vitamin B2, vitamin D and vitamin E. The compound traditional Chinese medicine polysaccharide is obtained by extraction from curculigo orchioides, eucommia ulmoides, poria cocos and dipsacus asperoids. After chick embryos are inoculated with the diluent, the virus resistance of the chick embryos can be improved, the death time is prolonged, and the virus multiplication time is prolonged. Compared with the prior art, the virus titer is improved by twice to eight times. According to the prepared diluent, the low-day-old chick embryos are inoculated, it is incidentally found that the size of obtained allantoic fluid is greatly increased, and the size of the obtained allantoic fluid is increased by 30-40%.

Description

technical field [0001] The invention belongs to the technical field of veterinary biological products, and in particular relates to a chicken infectious bronchitis virus dilution and a preparation method thereof. Background technique [0002] Chicken infectious bronchitis (IB) is an acute, highly contagious viral disease of chickens caused by infectious bronchitis virus (IBV), characterized by tracheal rales, coughing, and sneezing. One of the main diseases that harm the poultry industry. There are many serotypes of chicken infectious bronchitis, and more than 30 serotypes have been reported so far. Different serotype strains have great differences in virulence, pathogenicity and tissue tropism. There is no or only one serotype among them. Partial cross-immunity and the emergence of new serotypes have brought many difficulties to the prevention of IBV. [0003] At present, there are three methods for virus culture: animal inoculation, tissue culture and chicken embryo cult...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N7/00
Inventor 沈建军张秀文李阳
Owner 浙江美保龙生物技术有限公司
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