Spawn preparation method by switching enoki mushroom liquid spawn culture to solid spawn culture prior to liquification
A technology of liquid strain and reliquefaction, which is applied in the field of seed production of Flammulina velutipes from liquid strain to solid and reliquefaction, can solve the problems of high cost equipment investment, high technical requirements, low cost, etc., and achieve simple production and high infection rate of bacteria Low, pulp tender effect
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[0021] The present invention will be further described below in conjunction with embodiment.
[0022] The first step of the method of the present invention: cultivating liquid strains, the specific steps are as follows.
[0023] (1-1) Production of liquid culture medium: The ingredients required for each 1000ml of liquid triangular flask culture medium are: 20g of sugar, 3.3g of soybean milk treasure, 0.66g of potassium dihydrogen phosphate, and 0.66g of magnesium sulfate. Weigh all kinds of raw materials and mix them evenly ;Put the prepared material into a 1000ml triangular flask, stir and dissolve it fully, put it into a rotor, and then use pure water to set the volume to 600ml, and the pH value is required to be 6.2 to 6.5; plug the filled triangular bottle Put on a tampon, and cover the tampon with tinfoil to ensure that the tinfoil wraps the tampon completely, then put it in an autoclave, sterilize at 121°C for 60 minutes and take it out; put the taken out triangular fla...
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