A kind of artificial seed preparation method of submerged plant bitter grass

Abstract

The invention discloses a method for preparing artificial seeds of a submerged plant, vallisneria natans. The method comprises the following steps: (1) washing seedlings of vallisneria natans by water to remove plankton on the surfaces of the seedlings; (2) cutting plant leaves and long roots, and washing remaining tail stems and few roots of the plant by distilled water; (3) putting the washed remains in a gel solution formed by mixing calcium chloride, starch, a Hoagland's nutrient solution of anhydrous magnesium sulfate and naphthylacetic acid; and (4) dropwise adding the gel solution containing the buds of vallisneria natans into a solution of sodium alga acid and magnesium sulfate for carrying out a curing reaction by a dropper, taking out the buds after the curing reaction, and rinsing by sterile distilled water, thereby obtaining the artificial seeds of vallisneria natans after the reaction is ended. According to the method, plant tissue culture is omitted, the method does not need to be performed under sterile conditions, and thus the preparation processes are greatly reduced; the Hoagland's nutrient solution serves as artificial endosperm, the cost is greatly reduced, the period is shortened, and the survival rate is high. Moreover, a hollow capsule coating system is adopted, a liquid envelope is formed around a propagule, and thus the plant propagule is well protected.

Description

technical field [0001] The invention relates to a method for propagating submerged plants, which is a method of applying artificial seed technology to the propagation of submerged plants, in particular to a method for making artificial seeds of the submerged plant Erythria chinensis, which belongs to the artificial seeds of the submerged plant Erythria chinensis Innovative techniques for production methods. Background technique [0002] Artificial seed technology is to embed somatic embryos or meristems (buds, callus, embryoids, etc.) Granules formed that can germinate and emerge under suitable conditions. In 1981, after Lawrence et al. studied the lyoglue embedding tape technology wrapped by cells of celery and celery baskets, there were more and more reports on artificial seeds. In 1987, my country began to incorporate artificial seeds into the national high-tech development plan (863 plan), and studied the artificial seeds of some economic crops such as rubber trees and...

AI Technical Summary

Problems solved by technology

However, this method first induces sprouting on a solid medium containing hormones, that is, induces sprouting by plant tissue culture methods, and the experimental conditions and operation requirements of plant tissue culture technology are high. The preparation method is loaded down with trivial details, high risk, long period, and Application value is limited

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