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Conjugated polyelectrolyte, and preparation method therefor and biological application thereof

A technology of conjugated polyelectrolytes and compounds, applied in the field of biochemistry, can solve the problems of fluorescent substances that need to be improved

Inactive Publication Date: 2016-02-24
SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] Therefore, the fluorescent substances currently used for cell imaging still need to be improved

Method used

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  • Conjugated polyelectrolyte, and preparation method therefor and biological application thereof
  • Conjugated polyelectrolyte, and preparation method therefor and biological application thereof
  • Conjugated polyelectrolyte, and preparation method therefor and biological application thereof

Examples

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preparation example Construction

[0047] The compound shown in Formula 1 and the compound shown in Formula 2 used in the method for preparing the above-mentioned conjugated polyelectrolyte according to the embodiment of the present invention can be purchased from the market or prepared by yourself. Specifically, through figure 1 and figure 2 The compound shown in formula 1 and compound shown in formula 2 are respectively prepared by the synthetic route shown, wherein, m is an integer of 2-12.

[0048] In a specific example of the present invention, the method for preparing the aforementioned conjugated polyelectrolyte according to the embodiment of the present invention may include the following steps: dissolving the compound shown in Formula 1 and the compound shown in Formula 2 in tetrahydrofuran / triethylene In the mixed solvent of amine (V / V=3 / 1), the resulting mixture was placed under argon atmosphere for 20min, then, tetrakistriphenylphosphopalladium and cuprous iodide were added to the mixture under ar...

Embodiment 1

[0055] Embodiment 1: Preparation of conjugated polyelectrolyte (compound shown in formula 4)

[0056]

[0057] Wherein, n is an integer of about 41.

[0058] The compound shown in formula 3 (80.0 mg, 0.192 mmol) and the compound shown in formula 2 (96.0 mg, 0.192 mmol) were dissolved in 32 mL of tetrahydrofuran / triethylamine (V / V=3 / 1) mixed solvent, the resulting The obtained reaction system was placed in an argon atmosphere for 20min, after that, 8.7mg tetrakistriphenylphosphopalladium (7.5μmol) and 8mg cuprous iodide (7.5μmol) were added to the reaction system under an argon atmosphere, and the resulting mixture React at 55°C for 24h. After the reaction, the obtained reaction liquid was poured into 200 mL of glacial ether, and a red precipitate was precipitated, which was filtered to obtain a red precipitate, and vacuum-dried to obtain a compound represented by formula 4 (PPET3-N1). The hydrogen spectrum of compound shown in formula 4 sees image 3 .

Embodiment 2

[0059] Embodiment 2: Preparation of conjugated polyelectrolyte (compound shown in formula 6)

[0060]

[0061] Wherein, n is an integer of about 27.

[0062] In this example, the compound (PPET3-N2) shown in formula 6 was prepared according to the same method as in Example 1, and the hydrogen spectrum of the compound shown in formula 6 was obtained in image 3 .

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Abstract

The present invention provides a conjugated polyelectrolyte, and a preparation method therefor and a biological application thereof. The conjugated polyelectrolyte has a chemical structure as shown in the formula I, wherein m is an integer whose value is 2-12, n is an integer whose value is 10-100, and Et is an ethly. The conjugated polyelectrolyte has good cell compatibility, low toxicity and excellent optical physical properties (such as high brightness and high optical stability), and is sensitive to ATP response, and can be effectively used for cell imaging or ATP semi-quantitative detection. The formula I is as shown in the specification.

Description

technical field [0001] The invention relates to the technical field of biochemistry, in particular to a polyconjugated electrolyte and its preparation method and application. Background technique [0002] Cell fluorescence imaging has attracted much attention as an efficient detection method in the field of biological and pharmaceutical sciences. Generally, fluorescent substances used for cell imaging should have good water solubility, high brightness, photostability and low toxicity. Among the numerous fluorescent substances, organic small molecule dyes, fluorescent proteins, and quantum dots are widely used in cell imaging and subcellular target detection, but they all have their own drawbacks and are difficult to meet the imaging requirements. For example, fluorescent proteins and organic dyes photodrift significantly, while the cytotoxicity of quantum dots has become its bottleneck. [0003] Therefore, the fluorescent substances currently used for cell imaging still ne...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G61/12G01N21/64C12Q1/02
CPCC08G61/123C08G2261/12C08G2261/143C08G2261/334C08G2261/522C08G2261/94G01N21/6428G01N33/5005
Inventor 蒋宇扬谭春燕谭英陈志方吴盼
Owner SHENZHEN GRADUATE SCHOOL TSINGHUA UNIV
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